Expression response of chalcone synthase gene to inducing conditions and its effect on flavonoids accumulation in two medicinal species of Anoectochilus
Abstract Anoectochilus roxburghii and Anoectochilus formasanus are the major species of genus Anoectochilus used in traditional Chinese medicine for their abundant content of flavonoids and some other medicinal constituents. In recent years, their wild resources are gradually exhausted due to over-c...
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| Format: | Article |
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Nature Portfolio
2019-12-01
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| Series: | Scientific Reports |
| Online Access: | https://doi.org/10.1038/s41598-019-56821-0 |
| _version_ | 1818852868541644800 |
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| author | Lin Yang Jun Cheng Zhang Jing Tao Qu Gang He Hao Qiang Yu Wan Chen Li Feng Ling Fu |
| author_facet | Lin Yang Jun Cheng Zhang Jing Tao Qu Gang He Hao Qiang Yu Wan Chen Li Feng Ling Fu |
| author_sort | Lin Yang |
| collection | DOAJ |
| description | Abstract Anoectochilus roxburghii and Anoectochilus formasanus are the major species of genus Anoectochilus used in traditional Chinese medicine for their abundant content of flavonoids and some other medicinal constituents. In recent years, their wild resources are gradually exhausted due to over-collection and ecological deterioration. Artificial cultivation and tissue culture are employed to increase production. In this study, the open reading frame, promoter and genomic sequences of the chalcone synthase (CHS) gene were cloned from these two species according to their transcriptome information, and used for expression analysis in response to the induction of phenylalanine, ultraviolet light and NaCl, and its effect investigation on accumulation of flavonoids. The results showed that the expression of the CHS genes was upregulated in response to these inductions and resulted in increasing accumulation of total flavonoids. However, the increased flavonoids induced by phenylalanine and ultraviolet light were mainly allocated into the anthocyanidin branch of flavonoids biosynthesis. Not only did it improved the medicinal value, but might have inhibitory effect on plant growth because of the increased malondialdehyde accumulation. Under the induction of appropriate concentration of NaCl, the medicinal constituents of flavonoids were increased without inhibition to plant growth. |
| first_indexed | 2024-12-19T07:27:46Z |
| format | Article |
| id | doaj.art-44b38f829b2a4aeb9228bc3e117fed82 |
| institution | Directory Open Access Journal |
| issn | 2045-2322 |
| language | English |
| last_indexed | 2024-12-19T07:27:46Z |
| publishDate | 2019-12-01 |
| publisher | Nature Portfolio |
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| series | Scientific Reports |
| spelling | doaj.art-44b38f829b2a4aeb9228bc3e117fed822022-12-21T20:30:48ZengNature PortfolioScientific Reports2045-23222019-12-019111010.1038/s41598-019-56821-0Expression response of chalcone synthase gene to inducing conditions and its effect on flavonoids accumulation in two medicinal species of AnoectochilusLin Yang0Jun Cheng Zhang1Jing Tao Qu2Gang He3Hao Qiang Yu4Wan Chen Li5Feng Ling Fu6Maize Research Institute, Sichuan Agricultural UniversityMedical Plant Exploitation and Utilization Engineering Research Center, Fujian Province University, Sanming UniversityMaize Research Institute, Sichuan Agricultural UniversityKey Laboratory of Medicinal and Edible Plants Resources Development of Sichuan Education Department, Sichuan Industrial Institute of Antibiotics, Chengdu UniversityMaize Research Institute, Sichuan Agricultural UniversityMaize Research Institute, Sichuan Agricultural UniversityMaize Research Institute, Sichuan Agricultural UniversityAbstract Anoectochilus roxburghii and Anoectochilus formasanus are the major species of genus Anoectochilus used in traditional Chinese medicine for their abundant content of flavonoids and some other medicinal constituents. In recent years, their wild resources are gradually exhausted due to over-collection and ecological deterioration. Artificial cultivation and tissue culture are employed to increase production. In this study, the open reading frame, promoter and genomic sequences of the chalcone synthase (CHS) gene were cloned from these two species according to their transcriptome information, and used for expression analysis in response to the induction of phenylalanine, ultraviolet light and NaCl, and its effect investigation on accumulation of flavonoids. The results showed that the expression of the CHS genes was upregulated in response to these inductions and resulted in increasing accumulation of total flavonoids. However, the increased flavonoids induced by phenylalanine and ultraviolet light were mainly allocated into the anthocyanidin branch of flavonoids biosynthesis. Not only did it improved the medicinal value, but might have inhibitory effect on plant growth because of the increased malondialdehyde accumulation. Under the induction of appropriate concentration of NaCl, the medicinal constituents of flavonoids were increased without inhibition to plant growth.https://doi.org/10.1038/s41598-019-56821-0 |
| spellingShingle | Lin Yang Jun Cheng Zhang Jing Tao Qu Gang He Hao Qiang Yu Wan Chen Li Feng Ling Fu Expression response of chalcone synthase gene to inducing conditions and its effect on flavonoids accumulation in two medicinal species of Anoectochilus Scientific Reports |
| title | Expression response of chalcone synthase gene to inducing conditions and its effect on flavonoids accumulation in two medicinal species of Anoectochilus |
| title_full | Expression response of chalcone synthase gene to inducing conditions and its effect on flavonoids accumulation in two medicinal species of Anoectochilus |
| title_fullStr | Expression response of chalcone synthase gene to inducing conditions and its effect on flavonoids accumulation in two medicinal species of Anoectochilus |
| title_full_unstemmed | Expression response of chalcone synthase gene to inducing conditions and its effect on flavonoids accumulation in two medicinal species of Anoectochilus |
| title_short | Expression response of chalcone synthase gene to inducing conditions and its effect on flavonoids accumulation in two medicinal species of Anoectochilus |
| title_sort | expression response of chalcone synthase gene to inducing conditions and its effect on flavonoids accumulation in two medicinal species of anoectochilus |
| url | https://doi.org/10.1038/s41598-019-56821-0 |
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