Chemical Characterization and Biological Activity of the Essential Oil from <i>Araucaria brasiliensis</i> Collected in Ecuador
The purpose of this study was to determine the chemical composition, physical properties, enantiomeric composition and cholinesterase inhibitory activity of the essential oil (EO) steam-distilled from the leaves of the plant <i>Araucaria brasiliensis</i> Loud. collected in Ecuador. The c...
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| Format: | Article |
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MDPI AG
2022-06-01
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| Series: | Molecules |
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| Online Access: | https://www.mdpi.com/1420-3049/27/12/3793 |
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| author | Diana Jaramillo James Calva Nicole Bec Christian Larroque Giovanni Vidari Chabaco Armijos |
| author_facet | Diana Jaramillo James Calva Nicole Bec Christian Larroque Giovanni Vidari Chabaco Armijos |
| author_sort | Diana Jaramillo |
| collection | DOAJ |
| description | The purpose of this study was to determine the chemical composition, physical properties, enantiomeric composition and cholinesterase inhibitory activity of the essential oil (EO) steam-distilled from the leaves of the plant <i>Araucaria brasiliensis</i> Loud. collected in Ecuador. The chemical composition was determined by gas chromatography coupled to mass spectrometry (GC-MS) analysis on two capillary GC columns (DB5-ms and HP-INNOWax). Thirty-three compounds were identified in the EO; the main compounds were beyerene (26.08%), kaurene (24.86%), myrcene (11.02%), α-pinene (9.99%) and 5,15-rosadiene (5.87%). Diterpene hydrocarbons (65.41%), followed by monoterpene hydrocarbons (21.11%), were the most representative components of the EO. Enantioselective analysis of the EO showed four pairs of enantiomeric compounds, α-pinene, camphene, γ-muurolene and δ-cadinene. In an in vitro assay, the EO showed moderate inhibitory activity towards the enzyme butyrylcholinesterase (BuChE) (95.7 µg/mL), while it was inactive towards acetylcholinesterase (AChE) (225.3 µg/mL). Further in vivo studies are needed to confirm the anticholinesterase potential of the EO. |
| first_indexed | 2024-03-09T22:56:03Z |
| format | Article |
| id | doaj.art-44b3f02f021e474f8d7d084965d16bd2 |
| institution | Directory Open Access Journal |
| issn | 1420-3049 |
| language | English |
| last_indexed | 2024-03-09T22:56:03Z |
| publishDate | 2022-06-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Molecules |
| spelling | doaj.art-44b3f02f021e474f8d7d084965d16bd22023-11-23T18:11:23ZengMDPI AGMolecules1420-30492022-06-012712379310.3390/molecules27123793Chemical Characterization and Biological Activity of the Essential Oil from <i>Araucaria brasiliensis</i> Collected in EcuadorDiana Jaramillo0James Calva1Nicole Bec2Christian Larroque3Giovanni Vidari4Chabaco Armijos5Departamento de Química, Universidad Técnica Particular de Loja, Loja 1101608, EcuadorDepartamento de Química, Universidad Técnica Particular de Loja, Loja 1101608, EcuadorInstitute for Regenerative Medicine and Biotherapy (IRMB), Université de Montpellier, INSERM, 34295 Montpellier, FranceInstitute for Regenerative Medicine and Biotherapy (IRMB), Université de Montpellier, INSERM, 34295 Montpellier, FranceMedical Analysis Department, Faculty of Applied Science, Tishk International University, Erbil 44001, IraqDepartamento de Química, Universidad Técnica Particular de Loja, Loja 1101608, EcuadorThe purpose of this study was to determine the chemical composition, physical properties, enantiomeric composition and cholinesterase inhibitory activity of the essential oil (EO) steam-distilled from the leaves of the plant <i>Araucaria brasiliensis</i> Loud. collected in Ecuador. The chemical composition was determined by gas chromatography coupled to mass spectrometry (GC-MS) analysis on two capillary GC columns (DB5-ms and HP-INNOWax). Thirty-three compounds were identified in the EO; the main compounds were beyerene (26.08%), kaurene (24.86%), myrcene (11.02%), α-pinene (9.99%) and 5,15-rosadiene (5.87%). Diterpene hydrocarbons (65.41%), followed by monoterpene hydrocarbons (21.11%), were the most representative components of the EO. Enantioselective analysis of the EO showed four pairs of enantiomeric compounds, α-pinene, camphene, γ-muurolene and δ-cadinene. In an in vitro assay, the EO showed moderate inhibitory activity towards the enzyme butyrylcholinesterase (BuChE) (95.7 µg/mL), while it was inactive towards acetylcholinesterase (AChE) (225.3 µg/mL). Further in vivo studies are needed to confirm the anticholinesterase potential of the EO.https://www.mdpi.com/1420-3049/27/12/3793<i>Araucaria brasiliensis</i>essential oilchemical characterizationenantioselective analysisAChEBuChE |
| spellingShingle | Diana Jaramillo James Calva Nicole Bec Christian Larroque Giovanni Vidari Chabaco Armijos Chemical Characterization and Biological Activity of the Essential Oil from <i>Araucaria brasiliensis</i> Collected in Ecuador Molecules <i>Araucaria brasiliensis</i> essential oil chemical characterization enantioselective analysis AChE BuChE |
| title | Chemical Characterization and Biological Activity of the Essential Oil from <i>Araucaria brasiliensis</i> Collected in Ecuador |
| title_full | Chemical Characterization and Biological Activity of the Essential Oil from <i>Araucaria brasiliensis</i> Collected in Ecuador |
| title_fullStr | Chemical Characterization and Biological Activity of the Essential Oil from <i>Araucaria brasiliensis</i> Collected in Ecuador |
| title_full_unstemmed | Chemical Characterization and Biological Activity of the Essential Oil from <i>Araucaria brasiliensis</i> Collected in Ecuador |
| title_short | Chemical Characterization and Biological Activity of the Essential Oil from <i>Araucaria brasiliensis</i> Collected in Ecuador |
| title_sort | chemical characterization and biological activity of the essential oil from i araucaria brasiliensis i collected in ecuador |
| topic | <i>Araucaria brasiliensis</i> essential oil chemical characterization enantioselective analysis AChE BuChE |
| url | https://www.mdpi.com/1420-3049/27/12/3793 |
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