IPA1 improves drought tolerance by activating SNAC1 in rice
Abstract Drought is a major abiotic stress to rice (Oryza sativa) during growth. Ideal Plant Architecture (IPA1), the first cloned gene controlling the ideal plant type in rice, has been reported to function in both ideal rice plant architecture and biotic resistance. Here, we report that the IPA1/O...
| Main Authors: | , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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BMC
2023-01-01
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| Series: | BMC Plant Biology |
| Subjects: | |
| Online Access: | https://doi.org/10.1186/s12870-023-04062-9 |
| _version_ | 1811175933143416832 |
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| author | Feihe Chen Haomin Zhang Hong Li Ling Lian Yidong Wei Yuelong Lin Lanning Wang Wei He Qiuhua Cai Hongguang Xie Hua Zhang Jianfu Zhang |
| author_facet | Feihe Chen Haomin Zhang Hong Li Ling Lian Yidong Wei Yuelong Lin Lanning Wang Wei He Qiuhua Cai Hongguang Xie Hua Zhang Jianfu Zhang |
| author_sort | Feihe Chen |
| collection | DOAJ |
| description | Abstract Drought is a major abiotic stress to rice (Oryza sativa) during growth. Ideal Plant Architecture (IPA1), the first cloned gene controlling the ideal plant type in rice, has been reported to function in both ideal rice plant architecture and biotic resistance. Here, we report that the IPA1/OsSPL14, encoding a transcriptional factor, positively regulates drought tolerance in rice. The IPA1 is constitutively expressed and regulated by H2O2, abscisic acid, NaCl and polyethylene glycol 6000 treatments in rice. Furthermore, the IPA1-knockout plants showed much greater accumulation of H2O2 as measured by 3,3′-diaminobenzidine staining in leaves compared with WT plants. Yeast one-hybrid, dual-luciferase and electrophoretic mobility shift assays indicated that the IPA1 directly activates the promoter of SNAC1. Expression of SNAC1 is significantly down-regulated in IPA1 knockout plants. Further investigation indicated that the IPA1 plays a positive role in drought-stress tolerance by inducing reactive oxygen species scavenging in rice. Together, these findings indicated that the IPA1 played important roles in drought tolerance by regulating SNAC1, thus activating the antioxidant system in rice. |
| first_indexed | 2024-04-10T19:44:00Z |
| format | Article |
| id | doaj.art-44b4f09b91e84e90ad0a0729341f1505 |
| institution | Directory Open Access Journal |
| issn | 1471-2229 |
| language | English |
| last_indexed | 2024-04-10T19:44:00Z |
| publishDate | 2023-01-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Plant Biology |
| spelling | doaj.art-44b4f09b91e84e90ad0a0729341f15052023-01-29T12:08:11ZengBMCBMC Plant Biology1471-22292023-01-0123111210.1186/s12870-023-04062-9IPA1 improves drought tolerance by activating SNAC1 in riceFeihe Chen0Haomin Zhang1Hong Li2Ling Lian3Yidong Wei4Yuelong Lin5Lanning Wang6Wei He7Qiuhua Cai8Hongguang Xie9Hua Zhang10Jianfu Zhang11Rice Research Institute, Fujian Academy of Agricultural SciencesRice Research Institute, Fujian Academy of Agricultural SciencesRice Research Institute, Fujian Academy of Agricultural SciencesRice Research Institute, Fujian Academy of Agricultural SciencesRice Research Institute, Fujian Academy of Agricultural SciencesRice Research Institute, Fujian Academy of Agricultural SciencesRice Research Institute, Fujian Academy of Agricultural SciencesRice Research Institute, Fujian Academy of Agricultural SciencesRice Research Institute, Fujian Academy of Agricultural SciencesRice Research Institute, Fujian Academy of Agricultural SciencesCollege of Agriculture, Fujian Agriculture and Forestry UniversityRice Research Institute, Fujian Academy of Agricultural SciencesAbstract Drought is a major abiotic stress to rice (Oryza sativa) during growth. Ideal Plant Architecture (IPA1), the first cloned gene controlling the ideal plant type in rice, has been reported to function in both ideal rice plant architecture and biotic resistance. Here, we report that the IPA1/OsSPL14, encoding a transcriptional factor, positively regulates drought tolerance in rice. The IPA1 is constitutively expressed and regulated by H2O2, abscisic acid, NaCl and polyethylene glycol 6000 treatments in rice. Furthermore, the IPA1-knockout plants showed much greater accumulation of H2O2 as measured by 3,3′-diaminobenzidine staining in leaves compared with WT plants. Yeast one-hybrid, dual-luciferase and electrophoretic mobility shift assays indicated that the IPA1 directly activates the promoter of SNAC1. Expression of SNAC1 is significantly down-regulated in IPA1 knockout plants. Further investigation indicated that the IPA1 plays a positive role in drought-stress tolerance by inducing reactive oxygen species scavenging in rice. Together, these findings indicated that the IPA1 played important roles in drought tolerance by regulating SNAC1, thus activating the antioxidant system in rice.https://doi.org/10.1186/s12870-023-04062-9IPA1Rice (Oryza sativa L.)Drought stressSNAC1Reactive oxygen species (ROS) |
| spellingShingle | Feihe Chen Haomin Zhang Hong Li Ling Lian Yidong Wei Yuelong Lin Lanning Wang Wei He Qiuhua Cai Hongguang Xie Hua Zhang Jianfu Zhang IPA1 improves drought tolerance by activating SNAC1 in rice BMC Plant Biology IPA1 Rice (Oryza sativa L.) Drought stress SNAC1 Reactive oxygen species (ROS) |
| title | IPA1 improves drought tolerance by activating SNAC1 in rice |
| title_full | IPA1 improves drought tolerance by activating SNAC1 in rice |
| title_fullStr | IPA1 improves drought tolerance by activating SNAC1 in rice |
| title_full_unstemmed | IPA1 improves drought tolerance by activating SNAC1 in rice |
| title_short | IPA1 improves drought tolerance by activating SNAC1 in rice |
| title_sort | ipa1 improves drought tolerance by activating snac1 in rice |
| topic | IPA1 Rice (Oryza sativa L.) Drought stress SNAC1 Reactive oxygen species (ROS) |
| url | https://doi.org/10.1186/s12870-023-04062-9 |
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