Comparative study on the biodegradation of chlorpyrifos-methyl by Bacillus megaterium CM-Z19 and Pseudomonas syringae CM-Z6
Abstract: The strains CM-Z19 and CM-Z6, which are capable of highly degrading chlorpyrifos-methyl, were isolated from soil. They were identified as Bacillus megaterium CM-Z19 and Pseudomonas syringae CM-Z6, respectively, based on the 16S rRNA and an analysis of their morphological, physiological and...
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| Format: | Article |
| Language: | English |
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Academia Brasileira de Ciências
2019-10-01
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| Series: | Anais da Academia Brasileira de Ciências |
| Subjects: | |
| Online Access: | http://www.scielo.br/pdf/aabc/v91n3/0001-3765-aabc-91-03-e20180694.pdf |
| _version_ | 1798016182857498624 |
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| author | JIANGWEI ZHU YAN ZHAO HONGHUA RUAN |
| author_facet | JIANGWEI ZHU YAN ZHAO HONGHUA RUAN |
| author_sort | JIANGWEI ZHU |
| collection | DOAJ |
| description | Abstract: The strains CM-Z19 and CM-Z6, which are capable of highly degrading chlorpyrifos-methyl, were isolated from soil. They were identified as Bacillus megaterium CM-Z19 and Pseudomonas syringae CM-Z6, respectively, based on the 16S rRNA and an analysis of their morphological, physiological and biochemical characteristics. The strain CM-Z19 showed 92.6% degradation of chlorpyrifos-methyl (100 mg/L) within 5 days of incubation, and the strain CM-Z6 was 99.1% under the same conditions. In addition, the degradation characteristics of the two strains were compared and studied, and the results showed that the strain CM-Z19 had higher phosphoesterase activity and ability to degrade the organophosphorus pesticide than did the strain CM-Z6. However, the strain CM-Z19 could not degrade its first hydrolysis metabolite 3,5,6-trichloro-2-pyridinol (TCP) and could not completely degrade chlorpyrifos-methyl. The strain CM-Z6 could effectively degrade TCP and could degrade chlorpyrifos-methyl more quickly than strain CM-Z19. |
| first_indexed | 2024-04-11T15:46:49Z |
| format | Article |
| id | doaj.art-44bb77fff8284df69e8c466db7646783 |
| institution | Directory Open Access Journal |
| issn | 1678-2690 |
| language | English |
| last_indexed | 2024-04-11T15:46:49Z |
| publishDate | 2019-10-01 |
| publisher | Academia Brasileira de Ciências |
| record_format | Article |
| series | Anais da Academia Brasileira de Ciências |
| spelling | doaj.art-44bb77fff8284df69e8c466db76467832022-12-22T04:15:31ZengAcademia Brasileira de CiênciasAnais da Academia Brasileira de Ciências1678-26902019-10-0191310.1590/0001-3765201920180694Comparative study on the biodegradation of chlorpyrifos-methyl by Bacillus megaterium CM-Z19 and Pseudomonas syringae CM-Z6JIANGWEI ZHUhttps://orcid.org/0000-0003-0913-3435YAN ZHAOHONGHUA RUANAbstract: The strains CM-Z19 and CM-Z6, which are capable of highly degrading chlorpyrifos-methyl, were isolated from soil. They were identified as Bacillus megaterium CM-Z19 and Pseudomonas syringae CM-Z6, respectively, based on the 16S rRNA and an analysis of their morphological, physiological and biochemical characteristics. The strain CM-Z19 showed 92.6% degradation of chlorpyrifos-methyl (100 mg/L) within 5 days of incubation, and the strain CM-Z6 was 99.1% under the same conditions. In addition, the degradation characteristics of the two strains were compared and studied, and the results showed that the strain CM-Z19 had higher phosphoesterase activity and ability to degrade the organophosphorus pesticide than did the strain CM-Z6. However, the strain CM-Z19 could not degrade its first hydrolysis metabolite 3,5,6-trichloro-2-pyridinol (TCP) and could not completely degrade chlorpyrifos-methyl. The strain CM-Z6 could effectively degrade TCP and could degrade chlorpyrifos-methyl more quickly than strain CM-Z19.http://www.scielo.br/pdf/aabc/v91n3/0001-3765-aabc-91-03-e20180694.pdfBacillus megateriumPseudomonas syringaechlorpyrifos-methyl356-trichloro-2-pyridinolbiodegradation |
| spellingShingle | JIANGWEI ZHU YAN ZHAO HONGHUA RUAN Comparative study on the biodegradation of chlorpyrifos-methyl by Bacillus megaterium CM-Z19 and Pseudomonas syringae CM-Z6 Anais da Academia Brasileira de Ciências Bacillus megaterium Pseudomonas syringae chlorpyrifos-methyl 3 5 6-trichloro-2-pyridinol biodegradation |
| title | Comparative study on the biodegradation of chlorpyrifos-methyl by Bacillus megaterium CM-Z19 and Pseudomonas syringae CM-Z6 |
| title_full | Comparative study on the biodegradation of chlorpyrifos-methyl by Bacillus megaterium CM-Z19 and Pseudomonas syringae CM-Z6 |
| title_fullStr | Comparative study on the biodegradation of chlorpyrifos-methyl by Bacillus megaterium CM-Z19 and Pseudomonas syringae CM-Z6 |
| title_full_unstemmed | Comparative study on the biodegradation of chlorpyrifos-methyl by Bacillus megaterium CM-Z19 and Pseudomonas syringae CM-Z6 |
| title_short | Comparative study on the biodegradation of chlorpyrifos-methyl by Bacillus megaterium CM-Z19 and Pseudomonas syringae CM-Z6 |
| title_sort | comparative study on the biodegradation of chlorpyrifos methyl by bacillus megaterium cm z19 and pseudomonas syringae cm z6 |
| topic | Bacillus megaterium Pseudomonas syringae chlorpyrifos-methyl 3 5 6-trichloro-2-pyridinol biodegradation |
| url | http://www.scielo.br/pdf/aabc/v91n3/0001-3765-aabc-91-03-e20180694.pdf |
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