Differences in Immune Characteristics and Related Gene Expression in Spleen among Ningxiang, Berkshire Breeds and Their Hybrid Pigs
To investigate the differential immunology in Ningxiang and Berkshire pigs and their F<sub>1</sub> offspring (F<sub>1</sub> offspring), physiological and biochemical indicators in the plasma and spleen were analyzed. Then, transcriptomic analysis of the spleen identified 1348...
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MDPI AG
2024-02-01
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| Series: | Genes |
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| Online Access: | https://www.mdpi.com/2073-4425/15/2/205 |
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| author | Gang Song Yuebo Zhang Hu Gao Yawei Fu Yue Chen Yulong Yin Kang Xu |
| author_facet | Gang Song Yuebo Zhang Hu Gao Yawei Fu Yue Chen Yulong Yin Kang Xu |
| author_sort | Gang Song |
| collection | DOAJ |
| description | To investigate the differential immunology in Ningxiang and Berkshire pigs and their F<sub>1</sub> offspring (F<sub>1</sub> offspring), physiological and biochemical indicators in the plasma and spleen were analyzed. Then, transcriptomic analysis of the spleen identified 1348, 408, and 207 differentially expressed genes (DEGs) in comparisons of Ningxiang vs. Berkshire, Berkshire vs. F<sub>1</sub> offspring, and Ningxiang vs. F<sub>1</sub> offspring, respectively. In Ningxiang vs. Berkshire pigs, the gene ontology (GO) and the Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis indicated that the DEGs included <i>CD163</i>, <i>MARCO</i>, <i>CXCL14</i>, <i>CCL19</i>, and <i>PPBP</i>, which are associated with immunity. GO and KEGG analyses were also conducted comparing F<sub>1</sub> offspring and their parents. The DEGs, including <i>BPIFB1</i>, <i>HAVCR2</i>, <i>CD163</i>, <i>DDX3X</i>, <i>CCR5</i>, and <i>ITGB3</i>, were enriched in immune-related pathways. Protein–protein interaction (PPI) analysis indicated that the <i>EGFR</i> and <i>ITGA2</i> genes were key hub genes. In conclusion, this study identifies significant immune DEGs in different pig breeds, providing data to support the exploration of breeding strategies for disease resistance in local and crossbred pig populations. |
| first_indexed | 2024-03-07T22:31:30Z |
| format | Article |
| id | doaj.art-44bbf9ec84254594b58c28aa095864cb |
| institution | Directory Open Access Journal |
| issn | 2073-4425 |
| language | English |
| last_indexed | 2024-03-07T22:31:30Z |
| publishDate | 2024-02-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Genes |
| spelling | doaj.art-44bbf9ec84254594b58c28aa095864cb2024-02-23T15:17:49ZengMDPI AGGenes2073-44252024-02-0115220510.3390/genes15020205Differences in Immune Characteristics and Related Gene Expression in Spleen among Ningxiang, Berkshire Breeds and Their Hybrid PigsGang Song0Yuebo Zhang1Hu Gao2Yawei Fu3Yue Chen4Yulong Yin5Kang Xu6College of Animal Science and Technology, Hunan Agricultural University, Changsha 410128, ChinaCollege of Animal Science and Technology, Hunan Agricultural University, Changsha 410128, ChinaCollege of Animal Science and Technology, Hunan Agricultural University, Changsha 410128, ChinaCollege of Animal Science and Technology, Hunan Agricultural University, Changsha 410128, ChinaGuangdong Laboratory for Lingnan Modern Agriculture, Guangzhou 510642, ChinaGuangdong Laboratory for Lingnan Modern Agriculture, Guangzhou 510642, ChinaGuangdong Laboratory for Lingnan Modern Agriculture, Guangzhou 510642, ChinaTo investigate the differential immunology in Ningxiang and Berkshire pigs and their F<sub>1</sub> offspring (F<sub>1</sub> offspring), physiological and biochemical indicators in the plasma and spleen were analyzed. Then, transcriptomic analysis of the spleen identified 1348, 408, and 207 differentially expressed genes (DEGs) in comparisons of Ningxiang vs. Berkshire, Berkshire vs. F<sub>1</sub> offspring, and Ningxiang vs. F<sub>1</sub> offspring, respectively. In Ningxiang vs. Berkshire pigs, the gene ontology (GO) and the Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis indicated that the DEGs included <i>CD163</i>, <i>MARCO</i>, <i>CXCL14</i>, <i>CCL19</i>, and <i>PPBP</i>, which are associated with immunity. GO and KEGG analyses were also conducted comparing F<sub>1</sub> offspring and their parents. The DEGs, including <i>BPIFB1</i>, <i>HAVCR2</i>, <i>CD163</i>, <i>DDX3X</i>, <i>CCR5</i>, and <i>ITGB3</i>, were enriched in immune-related pathways. Protein–protein interaction (PPI) analysis indicated that the <i>EGFR</i> and <i>ITGA2</i> genes were key hub genes. In conclusion, this study identifies significant immune DEGs in different pig breeds, providing data to support the exploration of breeding strategies for disease resistance in local and crossbred pig populations.https://www.mdpi.com/2073-4425/15/2/205spleenimmune differenceRNA-SeqmRNApig breedhybrid pig |
| spellingShingle | Gang Song Yuebo Zhang Hu Gao Yawei Fu Yue Chen Yulong Yin Kang Xu Differences in Immune Characteristics and Related Gene Expression in Spleen among Ningxiang, Berkshire Breeds and Their Hybrid Pigs Genes spleen immune difference RNA-Seq mRNA pig breed hybrid pig |
| title | Differences in Immune Characteristics and Related Gene Expression in Spleen among Ningxiang, Berkshire Breeds and Their Hybrid Pigs |
| title_full | Differences in Immune Characteristics and Related Gene Expression in Spleen among Ningxiang, Berkshire Breeds and Their Hybrid Pigs |
| title_fullStr | Differences in Immune Characteristics and Related Gene Expression in Spleen among Ningxiang, Berkshire Breeds and Their Hybrid Pigs |
| title_full_unstemmed | Differences in Immune Characteristics and Related Gene Expression in Spleen among Ningxiang, Berkshire Breeds and Their Hybrid Pigs |
| title_short | Differences in Immune Characteristics and Related Gene Expression in Spleen among Ningxiang, Berkshire Breeds and Their Hybrid Pigs |
| title_sort | differences in immune characteristics and related gene expression in spleen among ningxiang berkshire breeds and their hybrid pigs |
| topic | spleen immune difference RNA-Seq mRNA pig breed hybrid pig |
| url | https://www.mdpi.com/2073-4425/15/2/205 |
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