Identification of Newcastle disease virus subgenotype VII.2 in wild birds in Turkey
Abstract Background Newcastle disease viruses (NDVs) can spread across continents via migratory birds. Hence, we investigated the frequency of NDV in both non-migratory and birds migrating on the Black Sea-Mediterranean flyway, in Istanbul, Turkey. Birds were trapped using nets placed around the Kuc...
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| Format: | Article |
| Language: | English |
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BMC
2020-08-01
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| Series: | BMC Veterinary Research |
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| Online Access: | http://link.springer.com/article/10.1186/s12917-020-02503-3 |
| _version_ | 1819019334354206720 |
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| author | Nuri Turan Cemal Ozsemir Aysun Yilmaz Utku Y. Cizmecigil Ozge Aydin Ozge Erdogan Bamac Aydin Gurel Ahmet Kutukcu Kubra Ozsemir H. Emre Tali Besim H. Tali Semaha G. Yilmaz Mehmetcan Yaramanoglu B. Kaan Tekelioğlu Serhat Ozsoy Juergen A. Richt Munir Iqbal Huseyin Yilmaz |
| author_facet | Nuri Turan Cemal Ozsemir Aysun Yilmaz Utku Y. Cizmecigil Ozge Aydin Ozge Erdogan Bamac Aydin Gurel Ahmet Kutukcu Kubra Ozsemir H. Emre Tali Besim H. Tali Semaha G. Yilmaz Mehmetcan Yaramanoglu B. Kaan Tekelioğlu Serhat Ozsoy Juergen A. Richt Munir Iqbal Huseyin Yilmaz |
| author_sort | Nuri Turan |
| collection | DOAJ |
| description | Abstract Background Newcastle disease viruses (NDVs) can spread across continents via migratory birds. Hence, we investigated the frequency of NDV in both non-migratory and birds migrating on the Black Sea-Mediterranean flyway, in Istanbul, Turkey. Birds were trapped using nets placed around the Kucukcekmece lake Avcilar, Istanbul, in spring seasons of 2016 and 2018. In total, 297 birds belonging to 42 different species were trapped, categorized according to species and sex, and flocked oropharyngeal swabs were collected. In addition, flocked swabs were also collected from 115 mallards caught by hunters around Edirne and from 207 birds which had been treated in the Veterinary Faculty of Istanbul university-Cerrahpasa. Tissue samples were taken from dead wild birds brought by public to Veterinary Faculty. A total of 619 flocked oropharyngeal swabs were pooled into 206 samples. RNA was extracted from swabs and tissue samples. Real-time RT-PCR prob. assay was used to detect NDV-RNA in samples. Results There was no amplification in real time RT-PCR in samples taken from wild birds caught by traps. However, amplification of NDV-F gene was observed in oropharyngeal swabs taken from 2 waterfowls (Common Moorhen and Mallard), and in tissue samples taken from 2 little owls and 1 common kestrel. Sequencing and phylogenetic analyses of these 5 samples for NDV-F gene showed great similarity with NDV subgenotype VII.2 viruses. Analysis also showed that there is a high similarity with the F gene sequences previously reported from Turkey in 2012 and as well as the sequences from neighbouring countries Bulgaria and Georgia and geographically close country such as Pakistan. Although the strains found in this study are closely related, there is a relatively small degree of molecular divergence within 543 bp of F gene of the Turkish NDV isolate and strains detected in Israel, Pakistan, Iran, United Arab Emirates and Belgium. Conclusions Our findings revealed the presence of subgenotype VII.2 of NDVs in wild birds in north west of Turkey and demonstrated some degree of molecular evolution when compared to the earlier NDV-VII.2 isolate in Turkey. |
| first_indexed | 2024-12-21T03:33:40Z |
| format | Article |
| id | doaj.art-44bc58bef1604158a079a0181960c65a |
| institution | Directory Open Access Journal |
| issn | 1746-6148 |
| language | English |
| last_indexed | 2024-12-21T03:33:40Z |
| publishDate | 2020-08-01 |
| publisher | BMC |
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| series | BMC Veterinary Research |
| spelling | doaj.art-44bc58bef1604158a079a0181960c65a2022-12-21T19:17:23ZengBMCBMC Veterinary Research1746-61482020-08-011611810.1186/s12917-020-02503-3Identification of Newcastle disease virus subgenotype VII.2 in wild birds in TurkeyNuri Turan0Cemal Ozsemir1Aysun Yilmaz2Utku Y. Cizmecigil3Ozge Aydin4Ozge Erdogan Bamac5Aydin Gurel6Ahmet Kutukcu7Kubra Ozsemir8H. Emre Tali9Besim H. Tali10Semaha G. Yilmaz11Mehmetcan Yaramanoglu12B. Kaan Tekelioğlu13Serhat Ozsoy14Juergen A. Richt15Munir Iqbal16Huseyin Yilmaz17Department of Virology, Veterinary Faculty, University of Istanbul-Cerrahpasa, AvcilarDepartment of Virology, Veterinary Faculty, University of Istanbul-Cerrahpasa, AvcilarDepartment of Virology, Veterinary Faculty, University of Istanbul-Cerrahpasa, AvcilarDepartment of Virology, Veterinary Faculty, University of Istanbul-Cerrahpasa, AvcilarDepartment of Virology, Veterinary Faculty, University of Istanbul-Cerrahpasa, AvcilarDepartment of Pathology, Veterinary Faculty, University of Istanbul-CerrahpasaDepartment of Pathology, Veterinary Faculty, University of Istanbul-CerrahpasaDepartment of Virology, Veterinary Faculty, University of Istanbul-Cerrahpasa, AvcilarDepartment of Wild Animals and Ecology, Veterinary Faculty, University of Istanbul-CerrahpasaDepartment of Virology, Veterinary Faculty, University of Istanbul-Cerrahpasa, AvcilarDepartment of Virology, Veterinary Faculty, University of Istanbul-Cerrahpasa, AvcilarDepartment of Virology, Veterinary Faculty, University of Istanbul-Cerrahpasa, AvcilarDepartment of Virology, Veterinary Faculty, University of Istanbul-Cerrahpasa, AvcilarDepartment of Virology, Veterinary Faculty, University of CukurovaDepartment of Wild Animals and Ecology, Veterinary Faculty, University of Istanbul-CerrahpasaDepartment of Diagnostic Medicine and Pathobiology, College of Veterinary Medicine, Kansas State UniversityThe Pirbright InstituteDepartment of Virology, Veterinary Faculty, University of Istanbul-Cerrahpasa, AvcilarAbstract Background Newcastle disease viruses (NDVs) can spread across continents via migratory birds. Hence, we investigated the frequency of NDV in both non-migratory and birds migrating on the Black Sea-Mediterranean flyway, in Istanbul, Turkey. Birds were trapped using nets placed around the Kucukcekmece lake Avcilar, Istanbul, in spring seasons of 2016 and 2018. In total, 297 birds belonging to 42 different species were trapped, categorized according to species and sex, and flocked oropharyngeal swabs were collected. In addition, flocked swabs were also collected from 115 mallards caught by hunters around Edirne and from 207 birds which had been treated in the Veterinary Faculty of Istanbul university-Cerrahpasa. Tissue samples were taken from dead wild birds brought by public to Veterinary Faculty. A total of 619 flocked oropharyngeal swabs were pooled into 206 samples. RNA was extracted from swabs and tissue samples. Real-time RT-PCR prob. assay was used to detect NDV-RNA in samples. Results There was no amplification in real time RT-PCR in samples taken from wild birds caught by traps. However, amplification of NDV-F gene was observed in oropharyngeal swabs taken from 2 waterfowls (Common Moorhen and Mallard), and in tissue samples taken from 2 little owls and 1 common kestrel. Sequencing and phylogenetic analyses of these 5 samples for NDV-F gene showed great similarity with NDV subgenotype VII.2 viruses. Analysis also showed that there is a high similarity with the F gene sequences previously reported from Turkey in 2012 and as well as the sequences from neighbouring countries Bulgaria and Georgia and geographically close country such as Pakistan. Although the strains found in this study are closely related, there is a relatively small degree of molecular divergence within 543 bp of F gene of the Turkish NDV isolate and strains detected in Israel, Pakistan, Iran, United Arab Emirates and Belgium. Conclusions Our findings revealed the presence of subgenotype VII.2 of NDVs in wild birds in north west of Turkey and demonstrated some degree of molecular evolution when compared to the earlier NDV-VII.2 isolate in Turkey.http://link.springer.com/article/10.1186/s12917-020-02503-3Newcastle disease virusWild birdsReal time RT-PCRPhylogeneticTurkey |
| spellingShingle | Nuri Turan Cemal Ozsemir Aysun Yilmaz Utku Y. Cizmecigil Ozge Aydin Ozge Erdogan Bamac Aydin Gurel Ahmet Kutukcu Kubra Ozsemir H. Emre Tali Besim H. Tali Semaha G. Yilmaz Mehmetcan Yaramanoglu B. Kaan Tekelioğlu Serhat Ozsoy Juergen A. Richt Munir Iqbal Huseyin Yilmaz Identification of Newcastle disease virus subgenotype VII.2 in wild birds in Turkey BMC Veterinary Research Newcastle disease virus Wild birds Real time RT-PCR Phylogenetic Turkey |
| title | Identification of Newcastle disease virus subgenotype VII.2 in wild birds in Turkey |
| title_full | Identification of Newcastle disease virus subgenotype VII.2 in wild birds in Turkey |
| title_fullStr | Identification of Newcastle disease virus subgenotype VII.2 in wild birds in Turkey |
| title_full_unstemmed | Identification of Newcastle disease virus subgenotype VII.2 in wild birds in Turkey |
| title_short | Identification of Newcastle disease virus subgenotype VII.2 in wild birds in Turkey |
| title_sort | identification of newcastle disease virus subgenotype vii 2 in wild birds in turkey |
| topic | Newcastle disease virus Wild birds Real time RT-PCR Phylogenetic Turkey |
| url | http://link.springer.com/article/10.1186/s12917-020-02503-3 |
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