Optimization of ultrasound-assisted extraction of colchicine compound from Colchicum haussknechtii by using response surface methodology
In this research an ultrasound-assisted extraction (UAE) method was used for extraction of colchicine in root of Colchicum haussknechtii prior to high-performance liquid chromatography with UV detection. C. haussknechtii is used widely in traditional medicine for the treatment of various diseases. T...
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| Format: | Article |
| Language: | English |
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Elsevier
2017-04-01
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| Series: | Journal of the Saudi Society of Agricultural Sciences |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S1658077X1500020X |
| _version_ | 1818560442073612288 |
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| author | Saeid Khodadoust Hossain Sadeghi Arezou Amiri Pebdani Jamshid Mohammadi Amin Salehi |
| author_facet | Saeid Khodadoust Hossain Sadeghi Arezou Amiri Pebdani Jamshid Mohammadi Amin Salehi |
| author_sort | Saeid Khodadoust |
| collection | DOAJ |
| description | In this research an ultrasound-assisted extraction (UAE) method was used for extraction of colchicine in root of Colchicum haussknechtii prior to high-performance liquid chromatography with UV detection. C. haussknechtii is used widely in traditional medicine for the treatment of various diseases. The root of this plant is full of colchicine that is suitable for the treatment of gout and cirrhosis and applicable in plant breeding studies to produce polyploidy. The influence of variables on the extraction method was investigated by response surface methodology (RSM) and composite design (CCD) to achieve maximum extraction yield of colchicine from the root of C. haussknechtii. The most suitable condition for the extraction of colchicine was found to at 40 °C temperature, 32 min extraction time, and 70:30 v/v ethanol–water mixtures with 45:1 solvent-solid ratio. Obtained results showed that there is 1.2% colchicine in the root of C. haussknechtii, so this plant could be introduced as a rich source of colchicine. |
| first_indexed | 2024-12-14T00:38:30Z |
| format | Article |
| id | doaj.art-44be09020a5c488aa3bdc639c2aed352 |
| institution | Directory Open Access Journal |
| issn | 1658-077X |
| language | English |
| last_indexed | 2024-12-14T00:38:30Z |
| publishDate | 2017-04-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of the Saudi Society of Agricultural Sciences |
| spelling | doaj.art-44be09020a5c488aa3bdc639c2aed3522022-12-21T23:24:30ZengElsevierJournal of the Saudi Society of Agricultural Sciences1658-077X2017-04-0116216317010.1016/j.jssas.2015.05.004Optimization of ultrasound-assisted extraction of colchicine compound from Colchicum haussknechtii by using response surface methodologySaeid Khodadoust0Hossain Sadeghi1Arezou Amiri Pebdani2Jamshid Mohammadi3Amin Salehi4Department of Chemistry, Behbahan Khatam Alanbia University of Technology, Behbahan, IranMedicinal Plants Research Center, Yasuj University of Medical Science, Yasuj, IranBehbahan Faculty of Medical Sciences, Behbahan, IranMedicinal Plants Research Center, Yasuj University of Medical Science, Yasuj, IranDepartment of Agronomy, Factually of Agriculture, Yasouj University, Yasouj, IranIn this research an ultrasound-assisted extraction (UAE) method was used for extraction of colchicine in root of Colchicum haussknechtii prior to high-performance liquid chromatography with UV detection. C. haussknechtii is used widely in traditional medicine for the treatment of various diseases. The root of this plant is full of colchicine that is suitable for the treatment of gout and cirrhosis and applicable in plant breeding studies to produce polyploidy. The influence of variables on the extraction method was investigated by response surface methodology (RSM) and composite design (CCD) to achieve maximum extraction yield of colchicine from the root of C. haussknechtii. The most suitable condition for the extraction of colchicine was found to at 40 °C temperature, 32 min extraction time, and 70:30 v/v ethanol–water mixtures with 45:1 solvent-solid ratio. Obtained results showed that there is 1.2% colchicine in the root of C. haussknechtii, so this plant could be introduced as a rich source of colchicine.http://www.sciencedirect.com/science/article/pii/S1658077X1500020XColchicum haussknechtiiExtractionCentral composite designDesirability functionHigh performance liquid chromatography |
| spellingShingle | Saeid Khodadoust Hossain Sadeghi Arezou Amiri Pebdani Jamshid Mohammadi Amin Salehi Optimization of ultrasound-assisted extraction of colchicine compound from Colchicum haussknechtii by using response surface methodology Journal of the Saudi Society of Agricultural Sciences Colchicum haussknechtii Extraction Central composite design Desirability function High performance liquid chromatography |
| title | Optimization of ultrasound-assisted extraction of colchicine compound from Colchicum haussknechtii by using response surface methodology |
| title_full | Optimization of ultrasound-assisted extraction of colchicine compound from Colchicum haussknechtii by using response surface methodology |
| title_fullStr | Optimization of ultrasound-assisted extraction of colchicine compound from Colchicum haussknechtii by using response surface methodology |
| title_full_unstemmed | Optimization of ultrasound-assisted extraction of colchicine compound from Colchicum haussknechtii by using response surface methodology |
| title_short | Optimization of ultrasound-assisted extraction of colchicine compound from Colchicum haussknechtii by using response surface methodology |
| title_sort | optimization of ultrasound assisted extraction of colchicine compound from colchicum haussknechtii by using response surface methodology |
| topic | Colchicum haussknechtii Extraction Central composite design Desirability function High performance liquid chromatography |
| url | http://www.sciencedirect.com/science/article/pii/S1658077X1500020X |
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