Linkage of lncRNA CRNDE sponging miR-181a-5p with aggravated inflammation underlying sepsis
This investigation was performed to verify whether lncRNA CRNDE sponging miR-181a-5p was involved with sepsis-relevant inflammatory dysfunctions. Aggregately 136 sepsis patients and 151 healthy people were recruited, and their fasting peripheral blood was gathered to detect expressions of CRNDE and...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
SAGE Publishing
2020-02-01
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| Series: | Innate Immunity |
| Online Access: | https://doi.org/10.1177/1753425919880946 |
| _version_ | 1818532148153417728 |
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| author | Yijun Wang Ziqiang Xu Dongyou Yue Zhenhua Zeng Weijie Yuan Ke Xu |
| author_facet | Yijun Wang Ziqiang Xu Dongyou Yue Zhenhua Zeng Weijie Yuan Ke Xu |
| author_sort | Yijun Wang |
| collection | DOAJ |
| description | This investigation was performed to verify whether lncRNA CRNDE sponging miR-181a-5p was involved with sepsis-relevant inflammatory dysfunctions. Aggregately 136 sepsis patients and 151 healthy people were recruited, and their fasting peripheral blood was gathered to detect expressions of CRNDE and miR-181a-5p. In addition, THP-1 cells were transfected with si-CRNDE, miR-181a-5p mimic, pcDNA3.1-TLR4 and si-TLR4, and then sepsis-specific inflammatory cytokines within the cells were quantified. The sponging relationships between CRNDE and miR-181a-5p, as well as between miR-181a-5p and TLR4, were ascertained by means of luciferase reporter gene assay. The experimental results revealed that over-expressed CRNDE and under-expressed miR-181a-5p were associated with shortened lifespan of sepsis patients. Mechanically, si-CRNDE-1 and miR-181a-5p mimic were able to reverse the promoting effects of LPS on production of NF-kB, TNF-α, IL-1β and IL-6 by THP-1 cells. Moreover, the expressional change of miR-181a-5p in THP-1 cells was in part owing to its being sponged by CRNDE. Lastly, TLR4, subjected to targeted modification of miR-181a-5p, was capable of disturbing the contribution of CRNDE and miR-181a-5p to THP-1 cells’ release of NF-kB, TNF-α, IL-1β and IL-6. Collectively, the CRNDE/miR-181a-5p/TLR4 axis seemed to have potential in modifying sepsis-related inflammatory pathogenesis, which offered a direction for sepsis diagnosis and treatment. |
| first_indexed | 2024-12-11T17:41:44Z |
| format | Article |
| id | doaj.art-44c3cfdcb07a4f2dba50e6574012415f |
| institution | Directory Open Access Journal |
| issn | 1753-4259 1753-4267 |
| language | English |
| last_indexed | 2024-12-11T17:41:44Z |
| publishDate | 2020-02-01 |
| publisher | SAGE Publishing |
| record_format | Article |
| series | Innate Immunity |
| spelling | doaj.art-44c3cfdcb07a4f2dba50e6574012415f2022-12-22T00:56:30ZengSAGE PublishingInnate Immunity1753-42591753-42672020-02-012610.1177/1753425919880946Linkage of lncRNA CRNDE sponging miR-181a-5p with aggravated inflammation underlying sepsisYijun WangZiqiang XuDongyou YueZhenhua ZengWeijie YuanKe XuThis investigation was performed to verify whether lncRNA CRNDE sponging miR-181a-5p was involved with sepsis-relevant inflammatory dysfunctions. Aggregately 136 sepsis patients and 151 healthy people were recruited, and their fasting peripheral blood was gathered to detect expressions of CRNDE and miR-181a-5p. In addition, THP-1 cells were transfected with si-CRNDE, miR-181a-5p mimic, pcDNA3.1-TLR4 and si-TLR4, and then sepsis-specific inflammatory cytokines within the cells were quantified. The sponging relationships between CRNDE and miR-181a-5p, as well as between miR-181a-5p and TLR4, were ascertained by means of luciferase reporter gene assay. The experimental results revealed that over-expressed CRNDE and under-expressed miR-181a-5p were associated with shortened lifespan of sepsis patients. Mechanically, si-CRNDE-1 and miR-181a-5p mimic were able to reverse the promoting effects of LPS on production of NF-kB, TNF-α, IL-1β and IL-6 by THP-1 cells. Moreover, the expressional change of miR-181a-5p in THP-1 cells was in part owing to its being sponged by CRNDE. Lastly, TLR4, subjected to targeted modification of miR-181a-5p, was capable of disturbing the contribution of CRNDE and miR-181a-5p to THP-1 cells’ release of NF-kB, TNF-α, IL-1β and IL-6. Collectively, the CRNDE/miR-181a-5p/TLR4 axis seemed to have potential in modifying sepsis-related inflammatory pathogenesis, which offered a direction for sepsis diagnosis and treatment.https://doi.org/10.1177/1753425919880946 |
| spellingShingle | Yijun Wang Ziqiang Xu Dongyou Yue Zhenhua Zeng Weijie Yuan Ke Xu Linkage of lncRNA CRNDE sponging miR-181a-5p with aggravated inflammation underlying sepsis Innate Immunity |
| title | Linkage of lncRNA CRNDE sponging miR-181a-5p with aggravated inflammation underlying sepsis |
| title_full | Linkage of lncRNA CRNDE sponging miR-181a-5p with aggravated inflammation underlying sepsis |
| title_fullStr | Linkage of lncRNA CRNDE sponging miR-181a-5p with aggravated inflammation underlying sepsis |
| title_full_unstemmed | Linkage of lncRNA CRNDE sponging miR-181a-5p with aggravated inflammation underlying sepsis |
| title_short | Linkage of lncRNA CRNDE sponging miR-181a-5p with aggravated inflammation underlying sepsis |
| title_sort | linkage of lncrna crnde sponging mir 181a 5p with aggravated inflammation underlying sepsis |
| url | https://doi.org/10.1177/1753425919880946 |
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