Phylogenetic Diversity of Diazotrophs along an Experimental Nutrient Gradient in Mangrove Sediments
The diversity of diazotrophs was studied in the sediment of mangrove forests (Twin Cays, Belize) subjected to a long-term fertilization with nitrogen and phosphorus. Terminal Restriction Fragment Length Polymorphism (TRFLP) and cloning of PCR-amplified nifH genes were combined via in silico analysis...
| Main Authors: | , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2015-07-01
|
| Series: | Journal of Marine Science and Engineering |
| Subjects: | |
| Online Access: | http://www.mdpi.com/2077-1312/3/3/699 |
| _version_ | 1818560266142482432 |
|---|---|
| author | Isabel C. Romero Myrna E. Jacobson-Meyers Jed A. Fuhrman Douglas G. Capone |
| author_facet | Isabel C. Romero Myrna E. Jacobson-Meyers Jed A. Fuhrman Douglas G. Capone |
| author_sort | Isabel C. Romero |
| collection | DOAJ |
| description | The diversity of diazotrophs was studied in the sediment of mangrove forests (Twin Cays, Belize) subjected to a long-term fertilization with nitrogen and phosphorus. Terminal Restriction Fragment Length Polymorphism (TRFLP) and cloning of PCR-amplified nifH genes were combined via in silico analysis to assign clones to TRFLP-nifH phylotypes, as well as to characterize the occurrence of phylotypes in response to environmental conditions. Results indicated that mangrove sediments from Belize harbor a unique diazotrophic community with a low metabolic diversity dominated by sulfate reducers. The variability of potential nitrogen-fixing sulfate reducers was explained by several environmental parameters, primarily by the abundance of dead roots in the sediments, and the concentration of H2S in the pore-waters. This study describes the complexity of microbial communities within the mangrove sediments with specific functional groups varying along environmental gradients. |
| first_indexed | 2024-12-14T00:36:17Z |
| format | Article |
| id | doaj.art-44c43ca0dff14600bac984f97a12e1c7 |
| institution | Directory Open Access Journal |
| issn | 2077-1312 |
| language | English |
| last_indexed | 2024-12-14T00:36:17Z |
| publishDate | 2015-07-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Journal of Marine Science and Engineering |
| spelling | doaj.art-44c43ca0dff14600bac984f97a12e1c72022-12-21T23:24:38ZengMDPI AGJournal of Marine Science and Engineering2077-13122015-07-013369971910.3390/jmse3030699jmse3030699Phylogenetic Diversity of Diazotrophs along an Experimental Nutrient Gradient in Mangrove SedimentsIsabel C. Romero0Myrna E. Jacobson-Meyers1Jed A. Fuhrman2Douglas G. Capone3College of Marine Science, University of South Florida, 140 7th Avenue South, St. Petersburg, FL 33701, USAUniversity of Southern California, 3616 Trousdale Parkway, Los Angeles, CA 90089, USAUniversity of Southern California, 3616 Trousdale Parkway, Los Angeles, CA 90089, USAUniversity of Southern California, 3616 Trousdale Parkway, Los Angeles, CA 90089, USAThe diversity of diazotrophs was studied in the sediment of mangrove forests (Twin Cays, Belize) subjected to a long-term fertilization with nitrogen and phosphorus. Terminal Restriction Fragment Length Polymorphism (TRFLP) and cloning of PCR-amplified nifH genes were combined via in silico analysis to assign clones to TRFLP-nifH phylotypes, as well as to characterize the occurrence of phylotypes in response to environmental conditions. Results indicated that mangrove sediments from Belize harbor a unique diazotrophic community with a low metabolic diversity dominated by sulfate reducers. The variability of potential nitrogen-fixing sulfate reducers was explained by several environmental parameters, primarily by the abundance of dead roots in the sediments, and the concentration of H2S in the pore-waters. This study describes the complexity of microbial communities within the mangrove sediments with specific functional groups varying along environmental gradients.http://www.mdpi.com/2077-1312/3/3/699diazotrophsmangrove sedimentsphylogenetic analysisgeneralized additive models |
| spellingShingle | Isabel C. Romero Myrna E. Jacobson-Meyers Jed A. Fuhrman Douglas G. Capone Phylogenetic Diversity of Diazotrophs along an Experimental Nutrient Gradient in Mangrove Sediments Journal of Marine Science and Engineering diazotrophs mangrove sediments phylogenetic analysis generalized additive models |
| title | Phylogenetic Diversity of Diazotrophs along an Experimental Nutrient Gradient in Mangrove Sediments |
| title_full | Phylogenetic Diversity of Diazotrophs along an Experimental Nutrient Gradient in Mangrove Sediments |
| title_fullStr | Phylogenetic Diversity of Diazotrophs along an Experimental Nutrient Gradient in Mangrove Sediments |
| title_full_unstemmed | Phylogenetic Diversity of Diazotrophs along an Experimental Nutrient Gradient in Mangrove Sediments |
| title_short | Phylogenetic Diversity of Diazotrophs along an Experimental Nutrient Gradient in Mangrove Sediments |
| title_sort | phylogenetic diversity of diazotrophs along an experimental nutrient gradient in mangrove sediments |
| topic | diazotrophs mangrove sediments phylogenetic analysis generalized additive models |
| url | http://www.mdpi.com/2077-1312/3/3/699 |
| work_keys_str_mv | AT isabelcromero phylogeneticdiversityofdiazotrophsalonganexperimentalnutrientgradientinmangrovesediments AT myrnaejacobsonmeyers phylogeneticdiversityofdiazotrophsalonganexperimentalnutrientgradientinmangrovesediments AT jedafuhrman phylogeneticdiversityofdiazotrophsalonganexperimentalnutrientgradientinmangrovesediments AT douglasgcapone phylogeneticdiversityofdiazotrophsalonganexperimentalnutrientgradientinmangrovesediments |