Identification of blood meal sources of Lutzomyia longipalpis using polymerase chain reaction-restriction fragment length polymorphism analysis of the cytochrome B gene
An analysis of the dietary content of haematophagous insects can provide important information about the transmission networks of certain zoonoses. The present study evaluated the potential of polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) analysis of the mitochondrial...
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| Format: | Article |
| Language: | English |
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Fundação Oswaldo Cruz (FIOCRUZ)
2014-06-01
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| Series: | Memorias do Instituto Oswaldo Cruz |
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| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762014000300379&lng=en&tlng=en |
| _version_ | 1797721188762386432 |
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| author | Vítor Yamashiro Rocha Soares Jailthon Carlos da Silva Kleverton Ribeiro da Silva Maria do Socorro Pires e Cruz Marcos Pérsio Dantas Santos Paulo Eduardo Martins Ribolla Diego Peres Alonso Luiz Felipe Leomil Coelho Dorcas Lamounier Costa Carlos Henrique Nery Costa |
| author_facet | Vítor Yamashiro Rocha Soares Jailthon Carlos da Silva Kleverton Ribeiro da Silva Maria do Socorro Pires e Cruz Marcos Pérsio Dantas Santos Paulo Eduardo Martins Ribolla Diego Peres Alonso Luiz Felipe Leomil Coelho Dorcas Lamounier Costa Carlos Henrique Nery Costa |
| author_sort | Vítor Yamashiro Rocha Soares |
| collection | DOAJ |
| description | An analysis of the dietary content of haematophagous insects can provide important information about the transmission networks of certain zoonoses. The present study evaluated the potential of polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) analysis of the mitochondrial cytochrome B (cytb) gene to differentiate between vertebrate species that were identified as possible sources of sandfly meals. The complete cytb gene sequences of 11 vertebrate species available in the National Center for Biotechnology Information database were digested with Aci I, Alu I, Hae III and Rsa I restriction enzymes in silico using Restriction Mapper software. The cytb gene fragment (358 bp) was amplified from tissue samples of vertebrate species and the dietary contents of sandflies and digested with restriction enzymes. Vertebrate species presented a restriction fragment profile that differed from that of other species, with the exception of Canis familiaris and Cerdocyon thous. The 358 bp fragment was identified in 76 sandflies. Of these, 10 were evaluated using the restriction enzymes and the food sources were predicted for four: Homo sapiens (1), Bos taurus (1) and Equus caballus (2). Thus, the PCR-RFLP technique could be a potential method for identifying the food sources of arthropods. However, some points must be clarified regarding the applicability of the method, such as the extent of DNA degradation through intestinal digestion, the potential for multiple sources of blood meals and the need for greater knowledge regarding intraspecific variations in mtDNA. |
| first_indexed | 2024-03-12T09:31:03Z |
| format | Article |
| id | doaj.art-44c84a6e1d6b47779c66a6bc3bf9e304 |
| institution | Directory Open Access Journal |
| issn | 1678-8060 |
| language | English |
| last_indexed | 2024-03-12T09:31:03Z |
| publishDate | 2014-06-01 |
| publisher | Fundação Oswaldo Cruz (FIOCRUZ) |
| record_format | Article |
| series | Memorias do Instituto Oswaldo Cruz |
| spelling | doaj.art-44c84a6e1d6b47779c66a6bc3bf9e3042023-09-02T13:54:16ZengFundação Oswaldo Cruz (FIOCRUZ)Memorias do Instituto Oswaldo Cruz1678-80602014-06-01109337938310.1590/0074-0276130405S0074-02762014000300379Identification of blood meal sources of Lutzomyia longipalpis using polymerase chain reaction-restriction fragment length polymorphism analysis of the cytochrome B geneVítor Yamashiro Rocha SoaresJailthon Carlos da SilvaKleverton Ribeiro da SilvaMaria do Socorro Pires e CruzMarcos Pérsio Dantas SantosPaulo Eduardo Martins RibollaDiego Peres AlonsoLuiz Felipe Leomil CoelhoDorcas Lamounier CostaCarlos Henrique Nery CostaAn analysis of the dietary content of haematophagous insects can provide important information about the transmission networks of certain zoonoses. The present study evaluated the potential of polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) analysis of the mitochondrial cytochrome B (cytb) gene to differentiate between vertebrate species that were identified as possible sources of sandfly meals. The complete cytb gene sequences of 11 vertebrate species available in the National Center for Biotechnology Information database were digested with Aci I, Alu I, Hae III and Rsa I restriction enzymes in silico using Restriction Mapper software. The cytb gene fragment (358 bp) was amplified from tissue samples of vertebrate species and the dietary contents of sandflies and digested with restriction enzymes. Vertebrate species presented a restriction fragment profile that differed from that of other species, with the exception of Canis familiaris and Cerdocyon thous. The 358 bp fragment was identified in 76 sandflies. Of these, 10 were evaluated using the restriction enzymes and the food sources were predicted for four: Homo sapiens (1), Bos taurus (1) and Equus caballus (2). Thus, the PCR-RFLP technique could be a potential method for identifying the food sources of arthropods. However, some points must be clarified regarding the applicability of the method, such as the extent of DNA degradation through intestinal digestion, the potential for multiple sources of blood meals and the need for greater knowledge regarding intraspecific variations in mtDNA.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762014000300379&lng=en&tlng=enblood meal analysiscytochrome BPCR-RFLP |
| spellingShingle | Vítor Yamashiro Rocha Soares Jailthon Carlos da Silva Kleverton Ribeiro da Silva Maria do Socorro Pires e Cruz Marcos Pérsio Dantas Santos Paulo Eduardo Martins Ribolla Diego Peres Alonso Luiz Felipe Leomil Coelho Dorcas Lamounier Costa Carlos Henrique Nery Costa Identification of blood meal sources of Lutzomyia longipalpis using polymerase chain reaction-restriction fragment length polymorphism analysis of the cytochrome B gene Memorias do Instituto Oswaldo Cruz blood meal analysis cytochrome B PCR-RFLP |
| title | Identification of blood meal sources of Lutzomyia longipalpis using polymerase chain reaction-restriction fragment length polymorphism analysis of the cytochrome B gene |
| title_full | Identification of blood meal sources of Lutzomyia longipalpis using polymerase chain reaction-restriction fragment length polymorphism analysis of the cytochrome B gene |
| title_fullStr | Identification of blood meal sources of Lutzomyia longipalpis using polymerase chain reaction-restriction fragment length polymorphism analysis of the cytochrome B gene |
| title_full_unstemmed | Identification of blood meal sources of Lutzomyia longipalpis using polymerase chain reaction-restriction fragment length polymorphism analysis of the cytochrome B gene |
| title_short | Identification of blood meal sources of Lutzomyia longipalpis using polymerase chain reaction-restriction fragment length polymorphism analysis of the cytochrome B gene |
| title_sort | identification of blood meal sources of lutzomyia longipalpis using polymerase chain reaction restriction fragment length polymorphism analysis of the cytochrome b gene |
| topic | blood meal analysis cytochrome B PCR-RFLP |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762014000300379&lng=en&tlng=en |
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