Renal Endothelial Cell‐Targeted Extracellular Vesicles Protect the Kidney from Ischemic Injury
Abstract Endothelial cell injury plays a critical part in ischemic acute kidney injury (AKI) and participates in the progression of AKI. Targeting renal endothelial cell therapy may ameliorate vascular injury and further improve the prognosis of ischemic AKI. Here, P‐selectin as a biomarker of ische...
| Main Authors: | , , , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Wiley
2023-01-01
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| Series: | Advanced Science |
| Subjects: | |
| Online Access: | https://doi.org/10.1002/advs.202204626 |
| _version_ | 1797943361931313152 |
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| author | Kaiyue Zhang Rongrong Li Xiaoniao Chen Hongyu Yan Huifang Li Xiaotong Zhao Haoyan Huang Shang Chen Yue Liu Kai Wang Zhibo Han Zhong‐Chao Han Deling Kong Xiang‐Mei Chen Zongjin Li |
| author_facet | Kaiyue Zhang Rongrong Li Xiaoniao Chen Hongyu Yan Huifang Li Xiaotong Zhao Haoyan Huang Shang Chen Yue Liu Kai Wang Zhibo Han Zhong‐Chao Han Deling Kong Xiang‐Mei Chen Zongjin Li |
| author_sort | Kaiyue Zhang |
| collection | DOAJ |
| description | Abstract Endothelial cell injury plays a critical part in ischemic acute kidney injury (AKI) and participates in the progression of AKI. Targeting renal endothelial cell therapy may ameliorate vascular injury and further improve the prognosis of ischemic AKI. Here, P‐selectin as a biomarker of ischemic AKI in endothelial cells is identified and P‐selectin binding peptide (PBP)‐engineered extracellular vesicles (PBP‐EVs) with imaging and therapeutic functions are developed. The results show that PBP‐EVs exhibit a selective targeting tendency to injured kidneys, while providing spatiotemporal information for the early diagnosis of AKI by quantifying the expression of P‐selectin in the kidneys by molecular imaging. Meanwhile, PBP‐EVs reveal superior nephroprotective functions in the promotion of renal repair and inhibition of fibrosis by alleviating inflammatory infiltration, improving reparative angiogenesis, and ameliorating maladaptive repair of the renal parenchyma. In conclusion, PBP‐EVs, as an ischemic AKI theranostic system that is designed in this study, provide a spatiotemporal diagnosis in the early stages of AKI to help guide personalized therapy and exhibit superior nephroprotective effects, offering proof‐of‐concept data to design EV‐based theranostic strategies to promote renal recovery and further improve long‐term outcomes following AKI. |
| first_indexed | 2024-04-10T20:22:58Z |
| format | Article |
| id | doaj.art-44c8a925386845569c6ab48b2da5e7ad |
| institution | Directory Open Access Journal |
| issn | 2198-3844 |
| language | English |
| last_indexed | 2024-04-10T20:22:58Z |
| publishDate | 2023-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Advanced Science |
| spelling | doaj.art-44c8a925386845569c6ab48b2da5e7ad2023-01-25T13:47:49ZengWileyAdvanced Science2198-38442023-01-01103n/an/a10.1002/advs.202204626Renal Endothelial Cell‐Targeted Extracellular Vesicles Protect the Kidney from Ischemic InjuryKaiyue Zhang0Rongrong Li1Xiaoniao Chen2Hongyu Yan3Huifang Li4Xiaotong Zhao5Haoyan Huang6Shang Chen7Yue Liu8Kai Wang9Zhibo Han10Zhong‐Chao Han11Deling Kong12Xiang‐Mei Chen13Zongjin Li14School of Medicine Nankai University Tianjin 300071 ChinaSchool of Medicine Nankai University Tianjin 300071 ChinaBeijing Tongren Eye Center Beijing Tongren Hospital Capital Medical University Beijing 100730 ChinaThe Key Laboratory of Bioactive Materials Ministry of Education College of Life Sciences Nankai University Tianjin 300071 ChinaSchool of Medicine Nankai University Tianjin 300071 ChinaHenan Key Laboratory of Medical Tissue Regeneration Xinxiang Medical University Xinxiang Henan 453003 ChinaSchool of Medicine Nankai University Tianjin 300071 ChinaSchool of Medicine Nankai University Tianjin 300071 ChinaSchool of Medicine Nankai University Tianjin 300071 ChinaThe Key Laboratory of Bioactive Materials Ministry of Education College of Life Sciences Nankai University Tianjin 300071 ChinaJiangxi Engineering Research Center for Stem Cell Shangrao Jiangxi 334000 ChinaJiangxi Engineering Research Center for Stem Cell Shangrao Jiangxi 334000 ChinaThe Key Laboratory of Bioactive Materials Ministry of Education College of Life Sciences Nankai University Tianjin 300071 ChinaState Key Laboratory of Kidney Diseases Chinese PLA General Hospital Beijing 100853 ChinaSchool of Medicine Nankai University Tianjin 300071 ChinaAbstract Endothelial cell injury plays a critical part in ischemic acute kidney injury (AKI) and participates in the progression of AKI. Targeting renal endothelial cell therapy may ameliorate vascular injury and further improve the prognosis of ischemic AKI. Here, P‐selectin as a biomarker of ischemic AKI in endothelial cells is identified and P‐selectin binding peptide (PBP)‐engineered extracellular vesicles (PBP‐EVs) with imaging and therapeutic functions are developed. The results show that PBP‐EVs exhibit a selective targeting tendency to injured kidneys, while providing spatiotemporal information for the early diagnosis of AKI by quantifying the expression of P‐selectin in the kidneys by molecular imaging. Meanwhile, PBP‐EVs reveal superior nephroprotective functions in the promotion of renal repair and inhibition of fibrosis by alleviating inflammatory infiltration, improving reparative angiogenesis, and ameliorating maladaptive repair of the renal parenchyma. In conclusion, PBP‐EVs, as an ischemic AKI theranostic system that is designed in this study, provide a spatiotemporal diagnosis in the early stages of AKI to help guide personalized therapy and exhibit superior nephroprotective effects, offering proof‐of‐concept data to design EV‐based theranostic strategies to promote renal recovery and further improve long‐term outcomes following AKI.https://doi.org/10.1002/advs.202204626acute kidney injuryendothelial cellextracellular vesicleP‐selectin binding peptidetheranostics |
| spellingShingle | Kaiyue Zhang Rongrong Li Xiaoniao Chen Hongyu Yan Huifang Li Xiaotong Zhao Haoyan Huang Shang Chen Yue Liu Kai Wang Zhibo Han Zhong‐Chao Han Deling Kong Xiang‐Mei Chen Zongjin Li Renal Endothelial Cell‐Targeted Extracellular Vesicles Protect the Kidney from Ischemic Injury Advanced Science acute kidney injury endothelial cell extracellular vesicle P‐selectin binding peptide theranostics |
| title | Renal Endothelial Cell‐Targeted Extracellular Vesicles Protect the Kidney from Ischemic Injury |
| title_full | Renal Endothelial Cell‐Targeted Extracellular Vesicles Protect the Kidney from Ischemic Injury |
| title_fullStr | Renal Endothelial Cell‐Targeted Extracellular Vesicles Protect the Kidney from Ischemic Injury |
| title_full_unstemmed | Renal Endothelial Cell‐Targeted Extracellular Vesicles Protect the Kidney from Ischemic Injury |
| title_short | Renal Endothelial Cell‐Targeted Extracellular Vesicles Protect the Kidney from Ischemic Injury |
| title_sort | renal endothelial cell targeted extracellular vesicles protect the kidney from ischemic injury |
| topic | acute kidney injury endothelial cell extracellular vesicle P‐selectin binding peptide theranostics |
| url | https://doi.org/10.1002/advs.202204626 |
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