Genome assembly of a symbiotic balantidia (Balantidium ctenopharyngodoni) in fish hindgut
Abstract Balantidium ctenopharyngodoni is identified as the sole ciliate species that exclusively resides within the hindgut of grass carp with high prevalence and intensity. In this study, the successful cultivation of B. ctenopharyngodoni enabled us to collect enough cells for genome sequencing. C...
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| Format: | Article |
| Language: | English |
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Nature Portfolio
2024-03-01
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| Series: | Scientific Data |
| Online Access: | https://doi.org/10.1038/s41597-024-03142-1 |
| _version_ | 1797233818832207872 |
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| author | Weishan Zhao Jie Xiong Ming Li Xialian Bu Chuanqi Jiang Guangying Wang Jing Zhang Wenxiang Li Hong Zou Wei Miao Kai Chen Guitang Wang |
| author_facet | Weishan Zhao Jie Xiong Ming Li Xialian Bu Chuanqi Jiang Guangying Wang Jing Zhang Wenxiang Li Hong Zou Wei Miao Kai Chen Guitang Wang |
| author_sort | Weishan Zhao |
| collection | DOAJ |
| description | Abstract Balantidium ctenopharyngodoni is identified as the sole ciliate species that exclusively resides within the hindgut of grass carp with high prevalence and intensity. In this study, the successful cultivation of B. ctenopharyngodoni enabled us to collect enough cells for genome sequencing. Consequently, we acquired a high-quality genome assembly spanning 68.66 Mb, encompassing a total of 22,334 nanochromosomes. Furthermore, we predicted 29,348 protein-coding genes, and 95.5% of them was supported by the RNA-seq data. The trend of GC content in the subtelomeric regions of single-gene chromosomes was similar to other ciliates containing nanochromosomes. A large number of genes encoding carbohydrate-binding modules with affinities for starch and peptidoglycans was identified. The identification of mitochondrion-related organelles (MROs) within genome indicates its well-suited adaptation to the anaerobic conditions in the hindgut environment. In summary, our results will offer resources for understanding the genetic basis and molecular adaptations of balantidia to hindgut of herbivorous fish. |
| first_indexed | 2024-04-24T16:22:13Z |
| format | Article |
| id | doaj.art-44cb3bdc4ee34f8b81eac1a4450cbf65 |
| institution | Directory Open Access Journal |
| issn | 2052-4463 |
| language | English |
| last_indexed | 2024-04-24T16:22:13Z |
| publishDate | 2024-03-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Data |
| spelling | doaj.art-44cb3bdc4ee34f8b81eac1a4450cbf652024-03-31T11:10:14ZengNature PortfolioScientific Data2052-44632024-03-0111111010.1038/s41597-024-03142-1Genome assembly of a symbiotic balantidia (Balantidium ctenopharyngodoni) in fish hindgutWeishan Zhao0Jie Xiong1Ming Li2Xialian Bu3Chuanqi Jiang4Guangying Wang5Jing Zhang6Wenxiang Li7Hong Zou8Wei Miao9Kai Chen10Guitang Wang11Institute of Hydrobiology, Chinese Academy of SciencesInstitute of Hydrobiology, Chinese Academy of SciencesInstitute of Hydrobiology, Chinese Academy of SciencesInstitute of Hydrobiology, Chinese Academy of SciencesInstitute of Hydrobiology, Chinese Academy of SciencesInstitute of Hydrobiology, Chinese Academy of SciencesInstitute of Hydrobiology, Chinese Academy of SciencesInstitute of Hydrobiology, Chinese Academy of SciencesInstitute of Hydrobiology, Chinese Academy of SciencesInstitute of Hydrobiology, Chinese Academy of SciencesInstitute of Hydrobiology, Chinese Academy of SciencesInstitute of Hydrobiology, Chinese Academy of SciencesAbstract Balantidium ctenopharyngodoni is identified as the sole ciliate species that exclusively resides within the hindgut of grass carp with high prevalence and intensity. In this study, the successful cultivation of B. ctenopharyngodoni enabled us to collect enough cells for genome sequencing. Consequently, we acquired a high-quality genome assembly spanning 68.66 Mb, encompassing a total of 22,334 nanochromosomes. Furthermore, we predicted 29,348 protein-coding genes, and 95.5% of them was supported by the RNA-seq data. The trend of GC content in the subtelomeric regions of single-gene chromosomes was similar to other ciliates containing nanochromosomes. A large number of genes encoding carbohydrate-binding modules with affinities for starch and peptidoglycans was identified. The identification of mitochondrion-related organelles (MROs) within genome indicates its well-suited adaptation to the anaerobic conditions in the hindgut environment. In summary, our results will offer resources for understanding the genetic basis and molecular adaptations of balantidia to hindgut of herbivorous fish.https://doi.org/10.1038/s41597-024-03142-1 |
| spellingShingle | Weishan Zhao Jie Xiong Ming Li Xialian Bu Chuanqi Jiang Guangying Wang Jing Zhang Wenxiang Li Hong Zou Wei Miao Kai Chen Guitang Wang Genome assembly of a symbiotic balantidia (Balantidium ctenopharyngodoni) in fish hindgut Scientific Data |
| title | Genome assembly of a symbiotic balantidia (Balantidium ctenopharyngodoni) in fish hindgut |
| title_full | Genome assembly of a symbiotic balantidia (Balantidium ctenopharyngodoni) in fish hindgut |
| title_fullStr | Genome assembly of a symbiotic balantidia (Balantidium ctenopharyngodoni) in fish hindgut |
| title_full_unstemmed | Genome assembly of a symbiotic balantidia (Balantidium ctenopharyngodoni) in fish hindgut |
| title_short | Genome assembly of a symbiotic balantidia (Balantidium ctenopharyngodoni) in fish hindgut |
| title_sort | genome assembly of a symbiotic balantidia balantidium ctenopharyngodoni in fish hindgut |
| url | https://doi.org/10.1038/s41597-024-03142-1 |
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