High-Frequency Pulsed Electric Field Ablation in Beagle Model for Treatment of Prostate Cancer
Conventional irreversible electroporation (IRE) with low-frequency pulsed electric field (LF-PEF) is used to induce cell death; however, it has several disadvantages including a long procedure time and severe muscle contraction due to high-voltage electric field. This study investigates a novel IRE...
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| Format: | Article |
| Language: | English |
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MDPI AG
2022-10-01
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| Series: | Cancers |
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| Online Access: | https://www.mdpi.com/2072-6694/14/20/4987 |
| _version_ | 1797474564937089024 |
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| author | Seung Jeong Song Hee Kim Hongbae Kim Jeon Min Kang Yubeen Park Dong-Sung Won Ji Won Kim Dae Sung Ryu Chu Hui Zeng Jong Hoon Chung Bumjin Lim Jung-Hoon Park |
| author_facet | Seung Jeong Song Hee Kim Hongbae Kim Jeon Min Kang Yubeen Park Dong-Sung Won Ji Won Kim Dae Sung Ryu Chu Hui Zeng Jong Hoon Chung Bumjin Lim Jung-Hoon Park |
| author_sort | Seung Jeong |
| collection | DOAJ |
| description | Conventional irreversible electroporation (IRE) with low-frequency pulsed electric field (LF-PEF) is used to induce cell death; however, it has several disadvantages including a long procedure time and severe muscle contraction due to high-voltage electric field. This study investigates a novel IRE protocol with high-frequency pulsed electric field (HF-PEF) of 500 Hz repetition to ablate the prostate tissue in beagles for treatment of prostate cancer. A finite element analysis was performed to validate optimal electrical field strength for the procedure. In total, 12 beagles received HF-PEF of 500 Hz and were sacrificed at 4 h, 4 days, and 28 days (3 each). The remaining three beagles underwent sham procedure. The outcomes of HF-PEF were assessed by histological responses. HF-PEF successfully decellularized the prostate tissues 4 h after the treatment. The prostate glands, duct, and urethra were well preserved after IRE with HF-PEF. The ablated prostatic tissues were gradually regenerated and appeared similar to the original tissues 28 d after IRE with HF-PEF. Moreover, electrocardiography and hematology demonstrated that IRE with HF-PEF did not seriously affect the cardiac tissue. HF-PEF was effective and safe in the beagle prostate and effectively induced the ablation and gradually recovered with cellular regeneration. |
| first_indexed | 2024-03-09T20:31:55Z |
| format | Article |
| id | doaj.art-44cbc20983804322867fb5437f742f40 |
| institution | Directory Open Access Journal |
| issn | 2072-6694 |
| language | English |
| last_indexed | 2024-03-09T20:31:55Z |
| publishDate | 2022-10-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Cancers |
| spelling | doaj.art-44cbc20983804322867fb5437f742f402023-11-23T23:20:04ZengMDPI AGCancers2072-66942022-10-011420498710.3390/cancers14204987High-Frequency Pulsed Electric Field Ablation in Beagle Model for Treatment of Prostate CancerSeung Jeong0Song Hee Kim1Hongbae Kim2Jeon Min Kang3Yubeen Park4Dong-Sung Won5Ji Won Kim6Dae Sung Ryu7Chu Hui Zeng8Jong Hoon Chung9Bumjin Lim10Jung-Hoon Park11Department of Biosystems & Biomaterials Science and Engineering, Seoul National University, Seoul 08826, KoreaBiomedical Engineering Research Center, Asan Institute for Life Sciences, Asan Medical Center, 88 Olympic-ro 43-gil, Songpa-gu, Seoul 05505, KoreaDepartment of Biosystems & Biomaterials Science and Engineering, Seoul National University, Seoul 08826, KoreaBiomedical Engineering Research Center, Asan Institute for Life Sciences, Asan Medical Center, 88 Olympic-ro 43-gil, Songpa-gu, Seoul 05505, KoreaBiomedical Engineering Research Center, Asan Institute for Life Sciences, Asan Medical Center, 88 Olympic-ro 43-gil, Songpa-gu, Seoul 05505, KoreaBiomedical Engineering Research Center, Asan Institute for Life Sciences, Asan Medical Center, 88 Olympic-ro 43-gil, Songpa-gu, Seoul 05505, KoreaBiomedical Engineering Research Center, Asan Institute for Life Sciences, Asan Medical Center, 88 Olympic-ro 43-gil, Songpa-gu, Seoul 05505, KoreaBiomedical Engineering Research Center, Asan Institute for Life Sciences, Asan Medical Center, 88 Olympic-ro 43-gil, Songpa-gu, Seoul 05505, KoreaBiomedical Engineering Research Center, Asan Institute for Life Sciences, Asan Medical Center, 88 Olympic-ro 43-gil, Songpa-gu, Seoul 05505, KoreaDepartment of Biosystems & Biomaterials Science and Engineering, Seoul National University, Seoul 08826, KoreaDepartment of Urology, Asan Medical Center, University of Ulsan College of Medicine, 88 Olympic-ro 43-gil, Songpa-gu, Seoul 05505, KoreaBiomedical Engineering Research Center, Asan Institute for Life Sciences, Asan Medical Center, 88 Olympic-ro 43-gil, Songpa-gu, Seoul 05505, KoreaConventional irreversible electroporation (IRE) with low-frequency pulsed electric field (LF-PEF) is used to induce cell death; however, it has several disadvantages including a long procedure time and severe muscle contraction due to high-voltage electric field. This study investigates a novel IRE protocol with high-frequency pulsed electric field (HF-PEF) of 500 Hz repetition to ablate the prostate tissue in beagles for treatment of prostate cancer. A finite element analysis was performed to validate optimal electrical field strength for the procedure. In total, 12 beagles received HF-PEF of 500 Hz and were sacrificed at 4 h, 4 days, and 28 days (3 each). The remaining three beagles underwent sham procedure. The outcomes of HF-PEF were assessed by histological responses. HF-PEF successfully decellularized the prostate tissues 4 h after the treatment. The prostate glands, duct, and urethra were well preserved after IRE with HF-PEF. The ablated prostatic tissues were gradually regenerated and appeared similar to the original tissues 28 d after IRE with HF-PEF. Moreover, electrocardiography and hematology demonstrated that IRE with HF-PEF did not seriously affect the cardiac tissue. HF-PEF was effective and safe in the beagle prostate and effectively induced the ablation and gradually recovered with cellular regeneration.https://www.mdpi.com/2072-6694/14/20/4987irreversible electroporationhigh-frequency pulsed electric fieldprostate cancerdecellularizationregeneration |
| spellingShingle | Seung Jeong Song Hee Kim Hongbae Kim Jeon Min Kang Yubeen Park Dong-Sung Won Ji Won Kim Dae Sung Ryu Chu Hui Zeng Jong Hoon Chung Bumjin Lim Jung-Hoon Park High-Frequency Pulsed Electric Field Ablation in Beagle Model for Treatment of Prostate Cancer Cancers irreversible electroporation high-frequency pulsed electric field prostate cancer decellularization regeneration |
| title | High-Frequency Pulsed Electric Field Ablation in Beagle Model for Treatment of Prostate Cancer |
| title_full | High-Frequency Pulsed Electric Field Ablation in Beagle Model for Treatment of Prostate Cancer |
| title_fullStr | High-Frequency Pulsed Electric Field Ablation in Beagle Model for Treatment of Prostate Cancer |
| title_full_unstemmed | High-Frequency Pulsed Electric Field Ablation in Beagle Model for Treatment of Prostate Cancer |
| title_short | High-Frequency Pulsed Electric Field Ablation in Beagle Model for Treatment of Prostate Cancer |
| title_sort | high frequency pulsed electric field ablation in beagle model for treatment of prostate cancer |
| topic | irreversible electroporation high-frequency pulsed electric field prostate cancer decellularization regeneration |
| url | https://www.mdpi.com/2072-6694/14/20/4987 |
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