Structural Characterization and in Vitro Immunomodulatory Activity of Polysaccharides from Selenium-Enriched Cordyceps militaris
This study aimed to investigate the structural characteristics and biological activity of polysaccharides from selenium-enriched Cordyceps militaris. A homogeneous polysaccharide (SeCMP0.2) was isolated and purified from the fruiting bodies of selenium-enriched C. militaris by water extraction and a...
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| Format: | Article |
| Language: | English |
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China Food Publishing Company
2023-11-01
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| Series: | Shipin Kexue |
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| Online Access: | https://www.spkx.net.cn/fileup/1002-6630/PDF/2023-44-22-006.pdf |
| _version_ | 1797376200964833280 |
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| author | YAO Yanting, YANG Xiaobing, CHEN Xujie, CHEN Zhongzheng, LIN Xiaorong, ZHANG Yuanyuan, XIANG Jie, CHEN Shaodan, JIAO Chunwei, LI Bin, GAO Xiong |
| author_facet | YAO Yanting, YANG Xiaobing, CHEN Xujie, CHEN Zhongzheng, LIN Xiaorong, ZHANG Yuanyuan, XIANG Jie, CHEN Shaodan, JIAO Chunwei, LI Bin, GAO Xiong |
| author_sort | YAO Yanting, YANG Xiaobing, CHEN Xujie, CHEN Zhongzheng, LIN Xiaorong, ZHANG Yuanyuan, XIANG Jie, CHEN Shaodan, JIAO Chunwei, LI Bin, GAO Xiong |
| collection | DOAJ |
| description | This study aimed to investigate the structural characteristics and biological activity of polysaccharides from selenium-enriched Cordyceps militaris. A homogeneous polysaccharide (SeCMP0.2) was isolated and purified from the fruiting bodies of selenium-enriched C. militaris by water extraction and alcohol precipitation, followed by anion exchange column chromatography. The structural characteristics of SeCMP0.2 were characterized by high-performance gel permeation chromatography (HPGPC), high-performance liquid chromatography (HPLC), nuclear magnetic resonance spectroscopy (NMR), and atomic force microscopy (AFM). The immunoregulatory activity of SeCMP0.2 was evaluated by using RAW264.7 mouse macrophages. Results showed that the weight average molecular mass and selenium content of SeCMP0.2 were 440.7 kDa and 49.1 μg/g, respectively. SeCMP0.2, with a triple-helix conformation, was aggregated in aqueous solution. Moreover, it was a heteropolysaccharide mainly composed of galactose (53.1%), glucose (8.2%), and mannose (37.1%), containing both α- and β-type glycosidic linkages. The dominant residues in SeCMP0.2 including T-Galp-(1→, →2)-Galp-(1→, and →6)-Manp-(1→ accounted for 16.6%, 29.4% and 26.9%, respectively. Additionally, the in vitro immunomodulatory assays showed that SeCMP0.2 could significantly upregulate the mRNA expression levels of inducible nitric oxide synthetase (iNOS), tumor necrosis factor-α (TNF-α), interleukin-6 (IL-6), and interleukin-1β (IL-1β) by activating the mitogen-activated protein kinases (MAPKs) and nuclear factor-κB (NF-κB) signaling pathways, thereby exerting immunoregulatory activity. These results will lay the foundation for further development and utilization of selenium-enriched C. militaris resources. |
| first_indexed | 2024-03-08T19:35:12Z |
| format | Article |
| id | doaj.art-44cc4b62a7364f84b5cc3e9e913daa7d |
| institution | Directory Open Access Journal |
| issn | 1002-6630 |
| language | English |
| last_indexed | 2024-03-08T19:35:12Z |
| publishDate | 2023-11-01 |
| publisher | China Food Publishing Company |
| record_format | Article |
| series | Shipin Kexue |
| spelling | doaj.art-44cc4b62a7364f84b5cc3e9e913daa7d2023-12-26T06:45:18ZengChina Food Publishing CompanyShipin Kexue1002-66302023-11-014422394810.7506/spkx1002-6630-20230217-161Structural Characterization and in Vitro Immunomodulatory Activity of Polysaccharides from Selenium-Enriched Cordyceps militarisYAO Yanting, YANG Xiaobing, CHEN Xujie, CHEN Zhongzheng, LIN Xiaorong, ZHANG Yuanyuan, XIANG Jie, CHEN Shaodan, JIAO Chunwei, LI Bin, GAO Xiong0(1. College of Food Science, South China Agricultural University, Guangzhou 510642, China;2. Institute of Microbiology, State Key Laboratory of Applied Microbiology Southern China, Guangdong Provincial Key Laboratory of Microbial Safety and Health, Guangdong Academy of Sciences, Guangzhou 510070, China; 3. Guangdong Yuewei Edible Fungi Technology Co. Ltd., Guangzhou 510663, China; 4. Guangdong Yuewei Biotechnology Co. Ltd., Zhaoqing 526000, China)This study aimed to investigate the structural characteristics and biological activity of polysaccharides from selenium-enriched Cordyceps militaris. A homogeneous polysaccharide (SeCMP0.2) was isolated and purified from the fruiting bodies of selenium-enriched C. militaris by water extraction and alcohol precipitation, followed by anion exchange column chromatography. The structural characteristics of SeCMP0.2 were characterized by high-performance gel permeation chromatography (HPGPC), high-performance liquid chromatography (HPLC), nuclear magnetic resonance spectroscopy (NMR), and atomic force microscopy (AFM). The immunoregulatory activity of SeCMP0.2 was evaluated by using RAW264.7 mouse macrophages. Results showed that the weight average molecular mass and selenium content of SeCMP0.2 were 440.7 kDa and 49.1 μg/g, respectively. SeCMP0.2, with a triple-helix conformation, was aggregated in aqueous solution. Moreover, it was a heteropolysaccharide mainly composed of galactose (53.1%), glucose (8.2%), and mannose (37.1%), containing both α- and β-type glycosidic linkages. The dominant residues in SeCMP0.2 including T-Galp-(1→, →2)-Galp-(1→, and →6)-Manp-(1→ accounted for 16.6%, 29.4% and 26.9%, respectively. Additionally, the in vitro immunomodulatory assays showed that SeCMP0.2 could significantly upregulate the mRNA expression levels of inducible nitric oxide synthetase (iNOS), tumor necrosis factor-α (TNF-α), interleukin-6 (IL-6), and interleukin-1β (IL-1β) by activating the mitogen-activated protein kinases (MAPKs) and nuclear factor-κB (NF-κB) signaling pathways, thereby exerting immunoregulatory activity. These results will lay the foundation for further development and utilization of selenium-enriched C. militaris resources.https://www.spkx.net.cn/fileup/1002-6630/PDF/2023-44-22-006.pdfselenium-enriched cordyceps militaris; polysaccharide; structural characteristics; immunoregulatory activity |
| spellingShingle | YAO Yanting, YANG Xiaobing, CHEN Xujie, CHEN Zhongzheng, LIN Xiaorong, ZHANG Yuanyuan, XIANG Jie, CHEN Shaodan, JIAO Chunwei, LI Bin, GAO Xiong Structural Characterization and in Vitro Immunomodulatory Activity of Polysaccharides from Selenium-Enriched Cordyceps militaris Shipin Kexue selenium-enriched cordyceps militaris; polysaccharide; structural characteristics; immunoregulatory activity |
| title | Structural Characterization and in Vitro Immunomodulatory Activity of Polysaccharides from Selenium-Enriched Cordyceps militaris |
| title_full | Structural Characterization and in Vitro Immunomodulatory Activity of Polysaccharides from Selenium-Enriched Cordyceps militaris |
| title_fullStr | Structural Characterization and in Vitro Immunomodulatory Activity of Polysaccharides from Selenium-Enriched Cordyceps militaris |
| title_full_unstemmed | Structural Characterization and in Vitro Immunomodulatory Activity of Polysaccharides from Selenium-Enriched Cordyceps militaris |
| title_short | Structural Characterization and in Vitro Immunomodulatory Activity of Polysaccharides from Selenium-Enriched Cordyceps militaris |
| title_sort | structural characterization and in vitro immunomodulatory activity of polysaccharides from selenium enriched cordyceps militaris |
| topic | selenium-enriched cordyceps militaris; polysaccharide; structural characteristics; immunoregulatory activity |
| url | https://www.spkx.net.cn/fileup/1002-6630/PDF/2023-44-22-006.pdf |
| work_keys_str_mv | AT yaoyantingyangxiaobingchenxujiechenzhongzhenglinxiaorongzhangyuanyuanxiangjiechenshaodanjiaochunweilibingaoxiong structuralcharacterizationandinvitroimmunomodulatoryactivityofpolysaccharidesfromseleniumenrichedcordycepsmilitaris |