Temporally and spatially resolved molecular profiling in fingerprint analysis using indium vanadate nanosheets-assisted laser desorption ionization mass spectrometry
Abstract This study presents the first-ever synthesis of samarium-doped indium vanadate nanosheets (IVONSs:Sm) via microemulsion-mediated solvothermal method. The nanosheets were subsequently utilized as a nano-matrix in laser desorption/ionization mass spectrometry (LDI-MS). It was discovered that...
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| Format: | Article |
| Language: | English |
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BMC
2023-12-01
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| Series: | Journal of Nanobiotechnology |
| Subjects: | |
| Online Access: | https://doi.org/10.1186/s12951-023-02239-w |
| _version_ | 1797273379661676544 |
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| author | Yanli Zhu Jikai Wang Chengxiao Fu Shuangquan Liu Pragati Awasthi Pengfei Zeng Danjun Chen Yiyang Sun Ziyi Mo Hailing Liu |
| author_facet | Yanli Zhu Jikai Wang Chengxiao Fu Shuangquan Liu Pragati Awasthi Pengfei Zeng Danjun Chen Yiyang Sun Ziyi Mo Hailing Liu |
| author_sort | Yanli Zhu |
| collection | DOAJ |
| description | Abstract This study presents the first-ever synthesis of samarium-doped indium vanadate nanosheets (IVONSs:Sm) via microemulsion-mediated solvothermal method. The nanosheets were subsequently utilized as a nano-matrix in laser desorption/ionization mass spectrometry (LDI-MS). It was discovered that the as-synthesized IVONSs:Sm possessed the following advantages: improved mass spectrometry signal, minimal matrix-related background, and exceptional stability in negative-ion mode. These qualities overcame the limitations of conventional matrices and enabled the sensitive detection of small biomolecules such as fatty acids. The negative-ion LDI mechanism of IVONSs:Sm was examined through the implementation of density functional theory simulation. Using IVONSs:Sm-assisted LDI-MS, fingerprint recognitions based on morphology and chemical profiles of endogenous/exogenous compounds were also achieved. Notably, crucial characteristics such as the age of an individual’s fingerprints and their physical state could be assessed through the longitudinal monitoring of particular biomolecules (e.g., ascorbic acid, fatty acid) or the specific biomarker bilirubin glucuronide. Critical information pertinent to the identification of an individual would thus be facilitated by the analysis of the compounds underlying the fingerprint patterns. Graphical Abstract |
| first_indexed | 2024-03-07T14:42:35Z |
| format | Article |
| id | doaj.art-44cc8cd5a8254371b36c0eaa8e49ee08 |
| institution | Directory Open Access Journal |
| issn | 1477-3155 |
| language | English |
| last_indexed | 2024-03-07T14:42:35Z |
| publishDate | 2023-12-01 |
| publisher | BMC |
| record_format | Article |
| series | Journal of Nanobiotechnology |
| spelling | doaj.art-44cc8cd5a8254371b36c0eaa8e49ee082024-03-05T20:09:40ZengBMCJournal of Nanobiotechnology1477-31552023-12-0121111510.1186/s12951-023-02239-wTemporally and spatially resolved molecular profiling in fingerprint analysis using indium vanadate nanosheets-assisted laser desorption ionization mass spectrometryYanli Zhu0Jikai Wang1Chengxiao Fu2Shuangquan Liu3Pragati Awasthi4Pengfei Zeng5Danjun Chen6Yiyang Sun7Ziyi Mo8Hailing Liu9School of Resources and Environment, Hunan University of Technology and BusinessHunan Province Cooperative Innovation Center for Molecular Target New Drug Study, Institute of Pharmacy & Pharmacology, Hengyang Medical School, University of South ChinaThe First Affiliated Hospital, Department of Clinical Laboratory, Department of Pharmacy, Hengyang Clinical Pharmacology Research Center, Hengyang Medical School, University of South ChinaThe First Affiliated Hospital, Department of Clinical Laboratory, Department of Pharmacy, Hengyang Clinical Pharmacology Research Center, Hengyang Medical School, University of South ChinaState Key Laboratory of Silicon Materials & School of Materials Science and Engineering, Zhejiang UniversityHunan Province Cooperative Innovation Center for Molecular Target New Drug Study, Institute of Pharmacy & Pharmacology, Hengyang Medical School, University of South ChinaThe First Affiliated Hospital, Department of Clinical Laboratory, Department of Pharmacy, Hengyang Clinical Pharmacology Research Center, Hengyang Medical School, University of South ChinaHunan Province Cooperative Innovation Center for Molecular Target New Drug Study, Institute of Pharmacy & Pharmacology, Hengyang Medical School, University of South ChinaHunan Province Cooperative Innovation Center for Molecular Target New Drug Study, Institute of Pharmacy & Pharmacology, Hengyang Medical School, University of South ChinaDepartment of Respiratory and Critical Care Medicine, Renmin Hospital of Wuhan UniversityAbstract This study presents the first-ever synthesis of samarium-doped indium vanadate nanosheets (IVONSs:Sm) via microemulsion-mediated solvothermal method. The nanosheets were subsequently utilized as a nano-matrix in laser desorption/ionization mass spectrometry (LDI-MS). It was discovered that the as-synthesized IVONSs:Sm possessed the following advantages: improved mass spectrometry signal, minimal matrix-related background, and exceptional stability in negative-ion mode. These qualities overcame the limitations of conventional matrices and enabled the sensitive detection of small biomolecules such as fatty acids. The negative-ion LDI mechanism of IVONSs:Sm was examined through the implementation of density functional theory simulation. Using IVONSs:Sm-assisted LDI-MS, fingerprint recognitions based on morphology and chemical profiles of endogenous/exogenous compounds were also achieved. Notably, crucial characteristics such as the age of an individual’s fingerprints and their physical state could be assessed through the longitudinal monitoring of particular biomolecules (e.g., ascorbic acid, fatty acid) or the specific biomarker bilirubin glucuronide. Critical information pertinent to the identification of an individual would thus be facilitated by the analysis of the compounds underlying the fingerprint patterns. Graphical Abstracthttps://doi.org/10.1186/s12951-023-02239-wIndium vanadate nanosheetsMass spectrometry imagingIonization mechanismChemical mappingFingerprint analysis |
| spellingShingle | Yanli Zhu Jikai Wang Chengxiao Fu Shuangquan Liu Pragati Awasthi Pengfei Zeng Danjun Chen Yiyang Sun Ziyi Mo Hailing Liu Temporally and spatially resolved molecular profiling in fingerprint analysis using indium vanadate nanosheets-assisted laser desorption ionization mass spectrometry Journal of Nanobiotechnology Indium vanadate nanosheets Mass spectrometry imaging Ionization mechanism Chemical mapping Fingerprint analysis |
| title | Temporally and spatially resolved molecular profiling in fingerprint analysis using indium vanadate nanosheets-assisted laser desorption ionization mass spectrometry |
| title_full | Temporally and spatially resolved molecular profiling in fingerprint analysis using indium vanadate nanosheets-assisted laser desorption ionization mass spectrometry |
| title_fullStr | Temporally and spatially resolved molecular profiling in fingerprint analysis using indium vanadate nanosheets-assisted laser desorption ionization mass spectrometry |
| title_full_unstemmed | Temporally and spatially resolved molecular profiling in fingerprint analysis using indium vanadate nanosheets-assisted laser desorption ionization mass spectrometry |
| title_short | Temporally and spatially resolved molecular profiling in fingerprint analysis using indium vanadate nanosheets-assisted laser desorption ionization mass spectrometry |
| title_sort | temporally and spatially resolved molecular profiling in fingerprint analysis using indium vanadate nanosheets assisted laser desorption ionization mass spectrometry |
| topic | Indium vanadate nanosheets Mass spectrometry imaging Ionization mechanism Chemical mapping Fingerprint analysis |
| url | https://doi.org/10.1186/s12951-023-02239-w |
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