Constitutive expression of VviNAC17 transcription factor significantly induces the synthesis of flavonoids and other phenolics in transgenic grape berry cells
VviNAC17 is a grapevine transcription factor activated by ABA. Because ABA has been proposed as the main signal modulating the secondary metabolism in grape berry skins, here we postulated VviNAC17 as a positive regulator of secondary metabolism in grape cells. To validate the hypothesis, VviNAC17 w...
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Frontiers Media S.A.
2022-07-01
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| Series: | Frontiers in Plant Science |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fpls.2022.964621/full |
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| author | Hélder Badim Mariana Vale Marco Coelho Antonio Granell Hernâni Gerós Hernâni Gerós Artur Conde |
| author_facet | Hélder Badim Mariana Vale Marco Coelho Antonio Granell Hernâni Gerós Hernâni Gerós Artur Conde |
| author_sort | Hélder Badim |
| collection | DOAJ |
| description | VviNAC17 is a grapevine transcription factor activated by ABA. Because ABA has been proposed as the main signal modulating the secondary metabolism in grape berry skins, here we postulated VviNAC17 as a positive regulator of secondary metabolism in grape cells. To validate the hypothesis, VviNAC17 was constitutively and stably overexpressed in grape berry suspension-cultured cells of Gamay Fréaux cv. by Agrobacterium-mediated transformation. Targeted transcriptional analyses by qPCR showed that several genes involved the phenylpropanoid (VviPAL1), stilbenoid (VviSTS1) and flavonoid pathways (VviDFR, VviLAR1, VviANR, VviLDOX, and VviUFGT1), as well as anthocyanin vacuolar transport and accumulation (VviGST4 and VvMATE1) were significantly upregulated in VviNAC17-overexpressing transgenic cells, which translated in the stimulation of a number of enzymatic activities in those pathways. This was the case of phenylalanine ammonia lyase (PAL) and UDP-glucose:flavonoid 3-O-glucosyltransferase (UFGT) that were about 2-fold and 3.5-fold higher in VviNAC17-overexpressing cells than in control cells. VviNAC17-overexpressing cells accumulated significantly higher amounts of anthocyanins, proanthocyanidins, total flavonoids and total phenolics. These findings confirmed that VviNAC17 is an important positive regulator of secondary metabolism in grapevine contributing to the accumulation of important berry quality-related secondary metabolites. |
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| last_indexed | 2024-12-11T16:29:55Z |
| publishDate | 2022-07-01 |
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| series | Frontiers in Plant Science |
| spelling | doaj.art-44d0921c699e4ffcb2be01718b0fc1682022-12-22T00:58:38ZengFrontiers Media S.A.Frontiers in Plant Science1664-462X2022-07-011310.3389/fpls.2022.964621964621Constitutive expression of VviNAC17 transcription factor significantly induces the synthesis of flavonoids and other phenolics in transgenic grape berry cellsHélder Badim0Mariana Vale1Marco Coelho2Antonio Granell3Hernâni Gerós4Hernâni Gerós5Artur Conde6Department of Biology, Centre of Molecular and Environmental Biology, University of Minho, Braga, PortugalDepartment of Biology, Centre of Molecular and Environmental Biology, University of Minho, Braga, PortugalDepartment of Biology, Centre of Molecular and Environmental Biology, University of Minho, Braga, PortugalInstitute of Molecular and Cellular Biology of Plants, Spanish National Research Council (CSIC), Polytechnic University of Valencia, Valencia, SpainDepartment of Biology, Centre of Molecular and Environmental Biology, University of Minho, Braga, PortugalDepartment of Biological Engineering, Centre of Biological Engineering, University of Minho, Braga, PortugalDepartment of Biology, Centre of Molecular and Environmental Biology, University of Minho, Braga, PortugalVviNAC17 is a grapevine transcription factor activated by ABA. Because ABA has been proposed as the main signal modulating the secondary metabolism in grape berry skins, here we postulated VviNAC17 as a positive regulator of secondary metabolism in grape cells. To validate the hypothesis, VviNAC17 was constitutively and stably overexpressed in grape berry suspension-cultured cells of Gamay Fréaux cv. by Agrobacterium-mediated transformation. Targeted transcriptional analyses by qPCR showed that several genes involved the phenylpropanoid (VviPAL1), stilbenoid (VviSTS1) and flavonoid pathways (VviDFR, VviLAR1, VviANR, VviLDOX, and VviUFGT1), as well as anthocyanin vacuolar transport and accumulation (VviGST4 and VvMATE1) were significantly upregulated in VviNAC17-overexpressing transgenic cells, which translated in the stimulation of a number of enzymatic activities in those pathways. This was the case of phenylalanine ammonia lyase (PAL) and UDP-glucose:flavonoid 3-O-glucosyltransferase (UFGT) that were about 2-fold and 3.5-fold higher in VviNAC17-overexpressing cells than in control cells. VviNAC17-overexpressing cells accumulated significantly higher amounts of anthocyanins, proanthocyanidins, total flavonoids and total phenolics. These findings confirmed that VviNAC17 is an important positive regulator of secondary metabolism in grapevine contributing to the accumulation of important berry quality-related secondary metabolites.https://www.frontiersin.org/articles/10.3389/fpls.2022.964621/fullVviNAC17homologous expressionanthocyaninsflavonoidsphenolicssecondary metabolites |
| spellingShingle | Hélder Badim Mariana Vale Marco Coelho Antonio Granell Hernâni Gerós Hernâni Gerós Artur Conde Constitutive expression of VviNAC17 transcription factor significantly induces the synthesis of flavonoids and other phenolics in transgenic grape berry cells Frontiers in Plant Science VviNAC17 homologous expression anthocyanins flavonoids phenolics secondary metabolites |
| title | Constitutive expression of VviNAC17 transcription factor significantly induces the synthesis of flavonoids and other phenolics in transgenic grape berry cells |
| title_full | Constitutive expression of VviNAC17 transcription factor significantly induces the synthesis of flavonoids and other phenolics in transgenic grape berry cells |
| title_fullStr | Constitutive expression of VviNAC17 transcription factor significantly induces the synthesis of flavonoids and other phenolics in transgenic grape berry cells |
| title_full_unstemmed | Constitutive expression of VviNAC17 transcription factor significantly induces the synthesis of flavonoids and other phenolics in transgenic grape berry cells |
| title_short | Constitutive expression of VviNAC17 transcription factor significantly induces the synthesis of flavonoids and other phenolics in transgenic grape berry cells |
| title_sort | constitutive expression of vvinac17 transcription factor significantly induces the synthesis of flavonoids and other phenolics in transgenic grape berry cells |
| topic | VviNAC17 homologous expression anthocyanins flavonoids phenolics secondary metabolites |
| url | https://www.frontiersin.org/articles/10.3389/fpls.2022.964621/full |
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