Transcriptomic Profiling of Pleural Effusions: Differences in Malignant and Infectious Fluids
<i>Background and Objectives</i>: Different cellular and molecular processes are involved in the production of malignant and infectious pleural effusions. However, the underlying mechanisms responsible for these differences or their consequences remain incompletely understood. The object...
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MDPI AG
2024-03-01
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| Series: | Medicina |
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| Online Access: | https://www.mdpi.com/1648-9144/60/3/424 |
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| author | Lucía Zamora-Molina Eduardo García-Pachón Marta Amorós Julia Gijón-Martínez Judith Sánchez-Almendro Carlos Baeza-Martínez Luis Hernández-Blasco Antonio Galiana |
| author_facet | Lucía Zamora-Molina Eduardo García-Pachón Marta Amorós Julia Gijón-Martínez Judith Sánchez-Almendro Carlos Baeza-Martínez Luis Hernández-Blasco Antonio Galiana |
| author_sort | Lucía Zamora-Molina |
| collection | DOAJ |
| description | <i>Background and Objectives</i>: Different cellular and molecular processes are involved in the production of malignant and infectious pleural effusions. However, the underlying mechanisms responsible for these differences or their consequences remain incompletely understood. The objective of this study was to identify differences in gene expression in pleural exudates of malignant and infectious aetiology and establish the possible different biological processes involved in both situations. <i>Materials and Methods</i>: RNA transcriptomic analysis was performed on 46 pleural fluid samples obtained during diagnostic thoracocenteses from 46 patients. There were 35 exudates (19 malignant and 16 infectious effusions) and 11 transudates that were used as a reference control group. Differential gene expression analysis for both exudative groups was identified. An enrichment score using the Human Kegg Orthology database was used for establishing the biological processes associated with malignant and infectious pleural effusions. <i>Results</i>: When comparing malignant exudates with infectious effusions, 27 differentially expressed genes with statistical significance were identified. Network analysis showed ten different biological processes for malignant and for infectious pleural effusions. In malignant fluids, processes related to protein synthesis and processing predominate. In infectious exudates, biological processes in connection with ATP production prevail. <i>Conclusions</i>: This study demonstrates differentially expressed genes in malignant and infectious pleural effusions, which could have important implications in the search for diagnostic or prognostic biomarkers. In addition, for the first time, biological processes involved in these two causes of pleural exudates have been described. |
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| language | English |
| last_indexed | 2024-04-24T18:01:23Z |
| publishDate | 2024-03-01 |
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| series | Medicina |
| spelling | doaj.art-44d6cb2661764b12ae14eb9b29fee7552024-03-27T13:53:35ZengMDPI AGMedicina1010-660X1648-91442024-03-0160342410.3390/medicina60030424Transcriptomic Profiling of Pleural Effusions: Differences in Malignant and Infectious FluidsLucía Zamora-Molina0Eduardo García-Pachón1Marta Amorós2Julia Gijón-Martínez3Judith Sánchez-Almendro4Carlos Baeza-Martínez5Luis Hernández-Blasco6Antonio Galiana7Section of Respiratory Medicine, Hospital General Universitario de Elche, 03203 Elche, Alicante, SpainSection of Respiratory Medicine, Hospital General Universitario de Elche, 03203 Elche, Alicante, SpainDepartment of Microbiology-FISABIO, Hospital General Universitario de Elche, 03203 Elche, Alicante, SpainDepartment of Microbiology-FISABIO, Hospital General Universitario de Elche, 03203 Elche, Alicante, SpainDepartment of Microbiology-FISABIO, Hospital General Universitario de Elche, 03203 Elche, Alicante, SpainSection of Respiratory Medicine, Hospital General Universitario de Elche, 03203 Elche, Alicante, SpainDepartment of Clinical Medicine, Universidad Miguel Hernández de Elche, 03202 Elche, Alicante, SpainDepartment of Microbiology-FISABIO, Hospital General Universitario de Elche, 03203 Elche, Alicante, Spain<i>Background and Objectives</i>: Different cellular and molecular processes are involved in the production of malignant and infectious pleural effusions. However, the underlying mechanisms responsible for these differences or their consequences remain incompletely understood. The objective of this study was to identify differences in gene expression in pleural exudates of malignant and infectious aetiology and establish the possible different biological processes involved in both situations. <i>Materials and Methods</i>: RNA transcriptomic analysis was performed on 46 pleural fluid samples obtained during diagnostic thoracocenteses from 46 patients. There were 35 exudates (19 malignant and 16 infectious effusions) and 11 transudates that were used as a reference control group. Differential gene expression analysis for both exudative groups was identified. An enrichment score using the Human Kegg Orthology database was used for establishing the biological processes associated with malignant and infectious pleural effusions. <i>Results</i>: When comparing malignant exudates with infectious effusions, 27 differentially expressed genes with statistical significance were identified. Network analysis showed ten different biological processes for malignant and for infectious pleural effusions. In malignant fluids, processes related to protein synthesis and processing predominate. In infectious exudates, biological processes in connection with ATP production prevail. <i>Conclusions</i>: This study demonstrates differentially expressed genes in malignant and infectious pleural effusions, which could have important implications in the search for diagnostic or prognostic biomarkers. In addition, for the first time, biological processes involved in these two causes of pleural exudates have been described.https://www.mdpi.com/1648-9144/60/3/424biomarkershigh-throughput sequencinginflammationmRNApleural effusiontranscriptome |
| spellingShingle | Lucía Zamora-Molina Eduardo García-Pachón Marta Amorós Julia Gijón-Martínez Judith Sánchez-Almendro Carlos Baeza-Martínez Luis Hernández-Blasco Antonio Galiana Transcriptomic Profiling of Pleural Effusions: Differences in Malignant and Infectious Fluids Medicina biomarkers high-throughput sequencing inflammation mRNA pleural effusion transcriptome |
| title | Transcriptomic Profiling of Pleural Effusions: Differences in Malignant and Infectious Fluids |
| title_full | Transcriptomic Profiling of Pleural Effusions: Differences in Malignant and Infectious Fluids |
| title_fullStr | Transcriptomic Profiling of Pleural Effusions: Differences in Malignant and Infectious Fluids |
| title_full_unstemmed | Transcriptomic Profiling of Pleural Effusions: Differences in Malignant and Infectious Fluids |
| title_short | Transcriptomic Profiling of Pleural Effusions: Differences in Malignant and Infectious Fluids |
| title_sort | transcriptomic profiling of pleural effusions differences in malignant and infectious fluids |
| topic | biomarkers high-throughput sequencing inflammation mRNA pleural effusion transcriptome |
| url | https://www.mdpi.com/1648-9144/60/3/424 |
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