Indian Almond (<i>Terminalia catappa</i> Linn.) Leaf Extract Extends Lifespan by Improving Lipid Metabolism and Antioxidant Activity Dependent on AMPK Signaling Pathway in <i>Caenorhabditis elegans</i> under High-Glucose-Diet Conditions
This study aimed to evaluate the antioxidant and antiaging effects of Indian almond (<i>Terminalia catappa</i> Linn.) leaf extract (TCE) on high-glucose (GLU)-induced obese <i>Caenorhabditis elegans</i>. Since TCE contains high contents of flavonoids and phenolics, strong rad...
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MDPI AG
2023-12-01
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| Series: | Antioxidants |
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| Online Access: | https://www.mdpi.com/2076-3921/13/1/14 |
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| author | Yebin Kim Seul-bi Lee Myogyeong Cho Soojin Choe Miran Jang |
| author_facet | Yebin Kim Seul-bi Lee Myogyeong Cho Soojin Choe Miran Jang |
| author_sort | Yebin Kim |
| collection | DOAJ |
| description | This study aimed to evaluate the antioxidant and antiaging effects of Indian almond (<i>Terminalia catappa</i> Linn.) leaf extract (TCE) on high-glucose (GLU)-induced obese <i>Caenorhabditis elegans</i>. Since TCE contains high contents of flavonoids and phenolics, strong radical scavenging activity was confirmed in vitro. The stress-resistance effect of TCE was confirmed under thermal and oxidative stress conditions at nontoxic tested concentrations (6.25, 12.5, and 25 μg/mL). GLU at 2% caused lipid and reactive oxygen species (ROS) accumulation in <i>C. elegans</i>, and TCE inhibited lipid and ROS accumulation under both normal and 2% GLU conditions in a concentration-dependent manner. In addition, TCE proved to be effective in prolonging the lifespan of <i>C. elegans</i> under normal and 2% GLU conditions. The ROS reduction effect of TCE was abolished in mutants deficient in daf-16/FOXO and skn-1/Nrf-2. In addition, the lifespan-extending effect of TCE in these two mutants disappeared. The lifespan-extending effect was abolished even in atgl-1/ATGL-deficiency mutants. The TCE effect was reduced in aak-1/AMPK-deficient mutants and completely abolished under 2% GLU conditions. Therefore, the effect of prolonging lifespan by inhibiting lipid and ROS accumulation under the high GLU conditions of TCE is considered to be the result of atgl-1, daf-16, and skn-1 being downregulated by aak-1. These results suggest that the physiological potential of TCE contributes to antiaging under metabolic disorders. |
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| format | Article |
| id | doaj.art-44d83b948b01464e84118723d7be12f9 |
| institution | Directory Open Access Journal |
| issn | 2076-3921 |
| language | English |
| last_indexed | 2024-03-08T11:07:07Z |
| publishDate | 2023-12-01 |
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| record_format | Article |
| series | Antioxidants |
| spelling | doaj.art-44d83b948b01464e84118723d7be12f92024-01-26T14:39:32ZengMDPI AGAntioxidants2076-39212023-12-011311410.3390/antiox13010014Indian Almond (<i>Terminalia catappa</i> Linn.) Leaf Extract Extends Lifespan by Improving Lipid Metabolism and Antioxidant Activity Dependent on AMPK Signaling Pathway in <i>Caenorhabditis elegans</i> under High-Glucose-Diet ConditionsYebin Kim0Seul-bi Lee1Myogyeong Cho2Soojin Choe3Miran Jang4Department of Smart Food and Drug, Inje University, Gimhae 50834, Republic of KoreaDepartment of Smart Food and Drug, Inje University, Gimhae 50834, Republic of KoreaDepartment of Smart Food and Drug, Inje University, Gimhae 50834, Republic of KoreaDepartment of Food Technology and Nutrition, Inje University, Gimhae 50834, Republic of KoreaDepartment of Smart Food and Drug, Inje University, Gimhae 50834, Republic of KoreaThis study aimed to evaluate the antioxidant and antiaging effects of Indian almond (<i>Terminalia catappa</i> Linn.) leaf extract (TCE) on high-glucose (GLU)-induced obese <i>Caenorhabditis elegans</i>. Since TCE contains high contents of flavonoids and phenolics, strong radical scavenging activity was confirmed in vitro. The stress-resistance effect of TCE was confirmed under thermal and oxidative stress conditions at nontoxic tested concentrations (6.25, 12.5, and 25 μg/mL). GLU at 2% caused lipid and reactive oxygen species (ROS) accumulation in <i>C. elegans</i>, and TCE inhibited lipid and ROS accumulation under both normal and 2% GLU conditions in a concentration-dependent manner. In addition, TCE proved to be effective in prolonging the lifespan of <i>C. elegans</i> under normal and 2% GLU conditions. The ROS reduction effect of TCE was abolished in mutants deficient in daf-16/FOXO and skn-1/Nrf-2. In addition, the lifespan-extending effect of TCE in these two mutants disappeared. The lifespan-extending effect was abolished even in atgl-1/ATGL-deficiency mutants. The TCE effect was reduced in aak-1/AMPK-deficient mutants and completely abolished under 2% GLU conditions. Therefore, the effect of prolonging lifespan by inhibiting lipid and ROS accumulation under the high GLU conditions of TCE is considered to be the result of atgl-1, daf-16, and skn-1 being downregulated by aak-1. These results suggest that the physiological potential of TCE contributes to antiaging under metabolic disorders.https://www.mdpi.com/2076-3921/13/1/14Indian almond (<i>Terminalia catappa</i> Linn.)<i>Caenorhabditis elegans</i>high-glucose dietobesityantioxidant |
| spellingShingle | Yebin Kim Seul-bi Lee Myogyeong Cho Soojin Choe Miran Jang Indian Almond (<i>Terminalia catappa</i> Linn.) Leaf Extract Extends Lifespan by Improving Lipid Metabolism and Antioxidant Activity Dependent on AMPK Signaling Pathway in <i>Caenorhabditis elegans</i> under High-Glucose-Diet Conditions Antioxidants Indian almond (<i>Terminalia catappa</i> Linn.) <i>Caenorhabditis elegans</i> high-glucose diet obesity antioxidant |
| title | Indian Almond (<i>Terminalia catappa</i> Linn.) Leaf Extract Extends Lifespan by Improving Lipid Metabolism and Antioxidant Activity Dependent on AMPK Signaling Pathway in <i>Caenorhabditis elegans</i> under High-Glucose-Diet Conditions |
| title_full | Indian Almond (<i>Terminalia catappa</i> Linn.) Leaf Extract Extends Lifespan by Improving Lipid Metabolism and Antioxidant Activity Dependent on AMPK Signaling Pathway in <i>Caenorhabditis elegans</i> under High-Glucose-Diet Conditions |
| title_fullStr | Indian Almond (<i>Terminalia catappa</i> Linn.) Leaf Extract Extends Lifespan by Improving Lipid Metabolism and Antioxidant Activity Dependent on AMPK Signaling Pathway in <i>Caenorhabditis elegans</i> under High-Glucose-Diet Conditions |
| title_full_unstemmed | Indian Almond (<i>Terminalia catappa</i> Linn.) Leaf Extract Extends Lifespan by Improving Lipid Metabolism and Antioxidant Activity Dependent on AMPK Signaling Pathway in <i>Caenorhabditis elegans</i> under High-Glucose-Diet Conditions |
| title_short | Indian Almond (<i>Terminalia catappa</i> Linn.) Leaf Extract Extends Lifespan by Improving Lipid Metabolism and Antioxidant Activity Dependent on AMPK Signaling Pathway in <i>Caenorhabditis elegans</i> under High-Glucose-Diet Conditions |
| title_sort | indian almond i terminalia catappa i linn leaf extract extends lifespan by improving lipid metabolism and antioxidant activity dependent on ampk signaling pathway in i caenorhabditis elegans i under high glucose diet conditions |
| topic | Indian almond (<i>Terminalia catappa</i> Linn.) <i>Caenorhabditis elegans</i> high-glucose diet obesity antioxidant |
| url | https://www.mdpi.com/2076-3921/13/1/14 |
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