Distinct Ezrin Truncations Differentiate Metastases in Sentinel Lymph Nodes from Unaffected Lymph Node Tissues, from Primary Breast Tumors, and from Healthy Glandular Breast Tissues
BACKGROUND: Lymph node metastasis status is a prognostic factor for further lymph node involvement and for patient survival in breast cancer patients. Frozen section analysis of lymph nodes is a reliable method for detection of macro-metastases. However, this method is far less effective in detectin...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2018-02-01
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| Series: | Translational Oncology |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S1936523317302851 |
| _version_ | 1818365825498742784 |
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| author | Claudia Röwer Christian George Toralf Reimer Bernd Stengel Anngret Radtke Bernd Gerber Michael O. Glocker |
| author_facet | Claudia Röwer Christian George Toralf Reimer Bernd Stengel Anngret Radtke Bernd Gerber Michael O. Glocker |
| author_sort | Claudia Röwer |
| collection | DOAJ |
| description | BACKGROUND: Lymph node metastasis status is a prognostic factor for further lymph node involvement and for patient survival in breast cancer patients. Frozen section analysis of lymph nodes is a reliable method for detection of macro-metastases. However, this method is far less effective in detecting micro-metastases, requesting improved diagnostic procedures. METHODS: We investigated expression and truncation of ezrin in (i) sentinel lymph node metastases, (ii) unaffected axillary lymph nodes, (iii) primary breast tumors, and (iv) healthy glandular breast tissues using 2D gel electrophoresis, SDS-PAGE, and mass spectrometry in addition to Western blotting. RESULTS: Full-length ezrin (E1; amino acids 1–586) is present in all four investigated tissues. Two truncated ezrin forms, one missing about the first hundred amino acids (E2a) and the other lacking about 150 C-terminal amino acids (E2b) were detectable in primary tumor tissues and in sentinel lymph node metastases but not in glandular tissues. Strikingly, an ezrin truncation (E3) which consists approximately of amino acids 238–586 was found strongly expressed in all sentinel lymph node metastases. Moreover, an N-terminal ezrin fragment (E4) that consists approximately of amino acids 1–273 was identified in sentinel lymph node metastases as well. CONCLUSIONS: We show for the first time the existence of tissue-dependent specific ezrin truncations. The distinguished strong Western blot staining of ezrin E3 in sentinel lymph node metastases underlines its capability to substantiate the occurrence of lymph node (micro)metastases in breast cancer patients. |
| first_indexed | 2024-12-13T22:26:25Z |
| format | Article |
| id | doaj.art-44da9317bdf7462697ae508bc44173a8 |
| institution | Directory Open Access Journal |
| issn | 1936-5233 1944-7124 |
| language | English |
| last_indexed | 2024-12-13T22:26:25Z |
| publishDate | 2018-02-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Translational Oncology |
| spelling | doaj.art-44da9317bdf7462697ae508bc44173a82022-12-21T23:29:13ZengElsevierTranslational Oncology1936-52331944-71242018-02-0111111010.1016/j.tranon.2017.10.004Distinct Ezrin Truncations Differentiate Metastases in Sentinel Lymph Nodes from Unaffected Lymph Node Tissues, from Primary Breast Tumors, and from Healthy Glandular Breast TissuesClaudia Röwer0Christian George1Toralf Reimer2Bernd Stengel3Anngret Radtke4Bernd Gerber5Michael O. Glocker6Proteome Center Rostock, University of Rostock, Schillingallee 69, 18057 Rostock, GermanyProteome Center Rostock, University of Rostock, Schillingallee 69, 18057 Rostock, GermanyDepartment of Obstetrics and Gynecology, University of Rostock, Südring 81, 18059 Rostock, GermanyPartnerschaft der Fachärzte für Pathologie, Südstadt Clinical Center, Südring 81, 18059 Rostock, GermanyPartnerschaft der Fachärzte für Pathologie, Südstadt Clinical Center, Südring 81, 18059 Rostock, GermanyDepartment of Obstetrics and Gynecology, University of Rostock, Südring 81, 18059 Rostock, GermanyProteome Center Rostock, University of Rostock, Schillingallee 69, 18057 Rostock, GermanyBACKGROUND: Lymph node metastasis status is a prognostic factor for further lymph node involvement and for patient survival in breast cancer patients. Frozen section analysis of lymph nodes is a reliable method for detection of macro-metastases. However, this method is far less effective in detecting micro-metastases, requesting improved diagnostic procedures. METHODS: We investigated expression and truncation of ezrin in (i) sentinel lymph node metastases, (ii) unaffected axillary lymph nodes, (iii) primary breast tumors, and (iv) healthy glandular breast tissues using 2D gel electrophoresis, SDS-PAGE, and mass spectrometry in addition to Western blotting. RESULTS: Full-length ezrin (E1; amino acids 1–586) is present in all four investigated tissues. Two truncated ezrin forms, one missing about the first hundred amino acids (E2a) and the other lacking about 150 C-terminal amino acids (E2b) were detectable in primary tumor tissues and in sentinel lymph node metastases but not in glandular tissues. Strikingly, an ezrin truncation (E3) which consists approximately of amino acids 238–586 was found strongly expressed in all sentinel lymph node metastases. Moreover, an N-terminal ezrin fragment (E4) that consists approximately of amino acids 1–273 was identified in sentinel lymph node metastases as well. CONCLUSIONS: We show for the first time the existence of tissue-dependent specific ezrin truncations. The distinguished strong Western blot staining of ezrin E3 in sentinel lymph node metastases underlines its capability to substantiate the occurrence of lymph node (micro)metastases in breast cancer patients.http://www.sciencedirect.com/science/article/pii/S1936523317302851 |
| spellingShingle | Claudia Röwer Christian George Toralf Reimer Bernd Stengel Anngret Radtke Bernd Gerber Michael O. Glocker Distinct Ezrin Truncations Differentiate Metastases in Sentinel Lymph Nodes from Unaffected Lymph Node Tissues, from Primary Breast Tumors, and from Healthy Glandular Breast Tissues Translational Oncology |
| title | Distinct Ezrin Truncations Differentiate Metastases in Sentinel Lymph Nodes from Unaffected Lymph Node Tissues, from Primary Breast Tumors, and from Healthy Glandular Breast Tissues |
| title_full | Distinct Ezrin Truncations Differentiate Metastases in Sentinel Lymph Nodes from Unaffected Lymph Node Tissues, from Primary Breast Tumors, and from Healthy Glandular Breast Tissues |
| title_fullStr | Distinct Ezrin Truncations Differentiate Metastases in Sentinel Lymph Nodes from Unaffected Lymph Node Tissues, from Primary Breast Tumors, and from Healthy Glandular Breast Tissues |
| title_full_unstemmed | Distinct Ezrin Truncations Differentiate Metastases in Sentinel Lymph Nodes from Unaffected Lymph Node Tissues, from Primary Breast Tumors, and from Healthy Glandular Breast Tissues |
| title_short | Distinct Ezrin Truncations Differentiate Metastases in Sentinel Lymph Nodes from Unaffected Lymph Node Tissues, from Primary Breast Tumors, and from Healthy Glandular Breast Tissues |
| title_sort | distinct ezrin truncations differentiate metastases in sentinel lymph nodes from unaffected lymph node tissues from primary breast tumors and from healthy glandular breast tissues |
| url | http://www.sciencedirect.com/science/article/pii/S1936523317302851 |
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