Compact and robust dual-color linearly polarized illumination source for three-photon fluorescence imaging
The miniaturized femtosecond laser in near infrared-II region is the core equipment of three-photon microscopy. In this paper, we design a compact and robust illumination source that emits dual-color linearly polarized light for three-photon microscopy. Based on an all-polarization-maintaining passi...
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Format: | Article |
Language: | English |
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World Scientific Publishing
2023-09-01
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Series: | Journal of Innovative Optical Health Sciences |
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Online Access: | https://www.worldscientific.com/doi/10.1142/S179354582241005X |
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author | Jiazheng Song Yanyan Zhang Yuanshan Liu |
author_facet | Jiazheng Song Yanyan Zhang Yuanshan Liu |
author_sort | Jiazheng Song |
collection | DOAJ |
description | The miniaturized femtosecond laser in near infrared-II region is the core equipment of three-photon microscopy. In this paper, we design a compact and robust illumination source that emits dual-color linearly polarized light for three-photon microscopy. Based on an all-polarization-maintaining passive mode-locked fiber laser, we shift the center wavelength of the pulses to the 1.7[Formula: see text][Formula: see text]m band utilizing cascade Raman effect, thereby generate dual-wavelength pulses. To enhance clarity, the two wavelengths are separated through the graded-index multimode fiber. Then we obtain the dual-pulse sequences with 1639.4[Formula: see text]nm and 1683.7[Formula: see text]nm wavelengths, 920[Formula: see text]fs pulse duration, and 23.75[Formula: see text]MHz pulse repetition rate. The average power of the signal is 53.64[Formula: see text]mW, corresponding to a single pulse energy of 2.25[Formula: see text]nJ. This illumination source can be further amplified and compressed for three-photon fluorescence imaging, especially dual-color three-photon fluorescence imaging, making it an ideal option for biomedical applications. |
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institution | Directory Open Access Journal |
issn | 1793-5458 1793-7205 |
language | English |
last_indexed | 2024-03-12T00:40:07Z |
publishDate | 2023-09-01 |
publisher | World Scientific Publishing |
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series | Journal of Innovative Optical Health Sciences |
spelling | doaj.art-44dd71f2af5b45f5aed2145993d0e5ae2023-09-15T08:16:34ZengWorld Scientific PublishingJournal of Innovative Optical Health Sciences1793-54581793-72052023-09-01160510.1142/S179354582241005XCompact and robust dual-color linearly polarized illumination source for three-photon fluorescence imagingJiazheng Song0Yanyan Zhang1Yuanshan Liu2School of Artificial Intelligence, Optics and Electronics (iOPEN), Northwestern Polytechnical University, Xi’an, Shaanxi 710072, P. R. ChinaSchool of Artificial Intelligence, Optics and Electronics (iOPEN), Northwestern Polytechnical University, Xi’an, Shaanxi 710072, P. R. ChinaSchool of Artificial Intelligence, Optics and Electronics (iOPEN), Northwestern Polytechnical University, Xi’an, Shaanxi 710072, P. R. ChinaThe miniaturized femtosecond laser in near infrared-II region is the core equipment of three-photon microscopy. In this paper, we design a compact and robust illumination source that emits dual-color linearly polarized light for three-photon microscopy. Based on an all-polarization-maintaining passive mode-locked fiber laser, we shift the center wavelength of the pulses to the 1.7[Formula: see text][Formula: see text]m band utilizing cascade Raman effect, thereby generate dual-wavelength pulses. To enhance clarity, the two wavelengths are separated through the graded-index multimode fiber. Then we obtain the dual-pulse sequences with 1639.4[Formula: see text]nm and 1683.7[Formula: see text]nm wavelengths, 920[Formula: see text]fs pulse duration, and 23.75[Formula: see text]MHz pulse repetition rate. The average power of the signal is 53.64[Formula: see text]mW, corresponding to a single pulse energy of 2.25[Formula: see text]nJ. This illumination source can be further amplified and compressed for three-photon fluorescence imaging, especially dual-color three-photon fluorescence imaging, making it an ideal option for biomedical applications.https://www.worldscientific.com/doi/10.1142/S179354582241005XThree-photon fluorescence imagingillumination sourcedual-wavelength femtosecond pulsecascaded Raman effectgraded-index multimode fiber |
spellingShingle | Jiazheng Song Yanyan Zhang Yuanshan Liu Compact and robust dual-color linearly polarized illumination source for three-photon fluorescence imaging Journal of Innovative Optical Health Sciences Three-photon fluorescence imaging illumination source dual-wavelength femtosecond pulse cascaded Raman effect graded-index multimode fiber |
title | Compact and robust dual-color linearly polarized illumination source for three-photon fluorescence imaging |
title_full | Compact and robust dual-color linearly polarized illumination source for three-photon fluorescence imaging |
title_fullStr | Compact and robust dual-color linearly polarized illumination source for three-photon fluorescence imaging |
title_full_unstemmed | Compact and robust dual-color linearly polarized illumination source for three-photon fluorescence imaging |
title_short | Compact and robust dual-color linearly polarized illumination source for three-photon fluorescence imaging |
title_sort | compact and robust dual color linearly polarized illumination source for three photon fluorescence imaging |
topic | Three-photon fluorescence imaging illumination source dual-wavelength femtosecond pulse cascaded Raman effect graded-index multimode fiber |
url | https://www.worldscientific.com/doi/10.1142/S179354582241005X |
work_keys_str_mv | AT jiazhengsong compactandrobustdualcolorlinearlypolarizedilluminationsourceforthreephotonfluorescenceimaging AT yanyanzhang compactandrobustdualcolorlinearlypolarizedilluminationsourceforthreephotonfluorescenceimaging AT yuanshanliu compactandrobustdualcolorlinearlypolarizedilluminationsourceforthreephotonfluorescenceimaging |