The Immunomodulatory Effect Of Radiofrequency Electromagnetic Field On Serum Cytokine Levels In A Mouse Model Of Hindlimb Unloading
Objective: Neuroblastoma (NB) is one of the frequently observed malignant solid tumors of childhood and infancy, accounting for 15% of pediatric cancer deaths. Recently, the approach of differentiation therapy has shown considerable promise in effective treatment of NB patients. MiR-124 belongs to...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Royan Institute (ACECR), Tehran
2021-01-01
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| Series: | Cell Journal |
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| Online Access: | https://celljournal.org/journal/article/fulltext/the-immunomodulatory-effect-of-radiofrequency-electromagnetic-field-on-serum-cytokine-levels-in-a-mouse-model-of-hindlimb-unloading.pdf |
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| author | Sima Aghajari Sayed Mohammad Javad Mortazavi Mehdi Kalani Samaneh Nematolah Parham Habibzadeh Shirin Farjadian |
| author_facet | Sima Aghajari Sayed Mohammad Javad Mortazavi Mehdi Kalani Samaneh Nematolah Parham Habibzadeh Shirin Farjadian |
| author_sort | Sima Aghajari |
| collection | DOAJ |
| description | Objective: Neuroblastoma (NB) is one of the frequently observed malignant solid tumors of childhood and infancy,
accounting for 15% of pediatric cancer deaths. Recently, the approach of differentiation therapy has shown considerable
promise in effective treatment of NB patients. MiR-124 belongs to the nervous system-specific miRNAs that is increased
during neuronal differentiation and may be one of the potential therapeutic targets for the treatment of NB. However,
despite its well-established therapeutic potential, its efficient delivery to the targeted tumor cells is a challenging task.
Mesenchymal stem cells (MSCs) are multipotent adult progenitor cells that have antitumor properties, and they can
migrate to cancer cells and tumors. This study aimed to assess whether human adipose tissue-derived MSCs (hADMSCs)
have the potential to deliver exogenous miRNAs to NB cells to induce differentiation and decrease proliferation
of cancer cells.
Materials and Methods: In this experimental study, hAD-MSCs were isolated, cultured, and differentiated. The M17
human NB cell line were also cultured. A specific type of miRNAs, i.e., miR-124 was successfully delivered to M17 NB
cells with the aid of hAD-MSCs using the direct or indirect (exosome-based) contacts.
Results: It was shown that indirect delivery of miR-124 considerably decreased the proliferation of NB cells and
induced their differentiation.
Conclusion: The results suggest the use of delivered exogenous miRNAs by the derived exosomes from hAD-MSCs
as a novel cell-free stem cell-based therapy for NB cancer. |
| first_indexed | 2024-12-13T08:40:39Z |
| format | Article |
| id | doaj.art-44ddf84bc5ad4d90afe893cc4aa7b910 |
| institution | Directory Open Access Journal |
| issn | 2228-5806 2228-5814 |
| language | English |
| last_indexed | 2024-12-13T08:40:39Z |
| publishDate | 2021-01-01 |
| publisher | Royan Institute (ACECR), Tehran |
| record_format | Article |
| series | Cell Journal |
| spelling | doaj.art-44ddf84bc5ad4d90afe893cc4aa7b9102022-12-21T23:53:32ZengRoyan Institute (ACECR), TehranCell Journal2228-58062228-58142021-01-0122440140510.22074/cellj.2021.6856The Immunomodulatory Effect Of Radiofrequency Electromagnetic Field On Serum Cytokine Levels In A Mouse Model Of Hindlimb UnloadingSima Aghajari0Sayed Mohammad Javad Mortazavi1Mehdi Kalani2Samaneh Nematolah3Parham Habibzadeh4Shirin Farjadian5Department of Radiology, Shiraz University of Medical Sciences, Shiraz, IranDepartment of Medical Physics, Shiraz University of Medical Sciences, Shiraz, IranDepartment of Immunology, Shiraz University of Medical Sciences, Shiraz, IranDepartment of Biostatistics, Shiraz University of Medical Sciences, Shiraz, IranR&D Department, Persian BayanGene Research and Training Center, Shiraz, IranDepartment of Immunology, Shiraz University of Medical Sciences, Shiraz, Iran,Email:farjadsh@sums.ac.irObjective: Neuroblastoma (NB) is one of the frequently observed malignant solid tumors of childhood and infancy, accounting for 15% of pediatric cancer deaths. Recently, the approach of differentiation therapy has shown considerable promise in effective treatment of NB patients. MiR-124 belongs to the nervous system-specific miRNAs that is increased during neuronal differentiation and may be one of the potential therapeutic targets for the treatment of NB. However, despite its well-established therapeutic potential, its efficient delivery to the targeted tumor cells is a challenging task. Mesenchymal stem cells (MSCs) are multipotent adult progenitor cells that have antitumor properties, and they can migrate to cancer cells and tumors. This study aimed to assess whether human adipose tissue-derived MSCs (hADMSCs) have the potential to deliver exogenous miRNAs to NB cells to induce differentiation and decrease proliferation of cancer cells. Materials and Methods: In this experimental study, hAD-MSCs were isolated, cultured, and differentiated. The M17 human NB cell line were also cultured. A specific type of miRNAs, i.e., miR-124 was successfully delivered to M17 NB cells with the aid of hAD-MSCs using the direct or indirect (exosome-based) contacts. Results: It was shown that indirect delivery of miR-124 considerably decreased the proliferation of NB cells and induced their differentiation. Conclusion: The results suggest the use of delivered exogenous miRNAs by the derived exosomes from hAD-MSCs as a novel cell-free stem cell-based therapy for NB cancer.https://celljournal.org/journal/article/fulltext/the-immunomodulatory-effect-of-radiofrequency-electromagnetic-field-on-serum-cytokine-levels-in-a-mouse-model-of-hindlimb-unloading.pdfdifferentiationexosomemesenchymal stem cellsmir-124 |
| spellingShingle | Sima Aghajari Sayed Mohammad Javad Mortazavi Mehdi Kalani Samaneh Nematolah Parham Habibzadeh Shirin Farjadian The Immunomodulatory Effect Of Radiofrequency Electromagnetic Field On Serum Cytokine Levels In A Mouse Model Of Hindlimb Unloading Cell Journal differentiation exosome mesenchymal stem cells mir-124 |
| title | The Immunomodulatory Effect Of Radiofrequency Electromagnetic Field On Serum Cytokine Levels In A Mouse Model Of Hindlimb Unloading |
| title_full | The Immunomodulatory Effect Of Radiofrequency Electromagnetic Field On Serum Cytokine Levels In A Mouse Model Of Hindlimb Unloading |
| title_fullStr | The Immunomodulatory Effect Of Radiofrequency Electromagnetic Field On Serum Cytokine Levels In A Mouse Model Of Hindlimb Unloading |
| title_full_unstemmed | The Immunomodulatory Effect Of Radiofrequency Electromagnetic Field On Serum Cytokine Levels In A Mouse Model Of Hindlimb Unloading |
| title_short | The Immunomodulatory Effect Of Radiofrequency Electromagnetic Field On Serum Cytokine Levels In A Mouse Model Of Hindlimb Unloading |
| title_sort | immunomodulatory effect of radiofrequency electromagnetic field on serum cytokine levels in a mouse model of hindlimb unloading |
| topic | differentiation exosome mesenchymal stem cells mir-124 |
| url | https://celljournal.org/journal/article/fulltext/the-immunomodulatory-effect-of-radiofrequency-electromagnetic-field-on-serum-cytokine-levels-in-a-mouse-model-of-hindlimb-unloading.pdf |
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