Novel water soluble dendrimer nanocarrier for enhanced photodynamic efficacy of protoporphyrin Ⅸ

The present study describes the development and evaluation of a novel biocompatible dendrimer-based nano drug delivery system which is readily soluble in water prepared by condensing Phloroglucinol and Succinic Acid (PGSA) and could efficiently encapsulate a well known hydrophobic photodynamic thera...

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Main Authors: Murugesan Suresh Kumar, Anish Babu, Ramachandran Murugesan, Kadarkaraithangam Jeyasubramanian
Format: Article
Language:English
Published: Tsinghua University Press 2012-09-01
Series:Nano Biomedicine and Engineering
Subjects:
Online Access:https://www.sciopen.com/article/10.5101/nbe.v4i3.p132-138
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author Murugesan Suresh Kumar
Anish Babu
Ramachandran Murugesan
Kadarkaraithangam Jeyasubramanian
author_facet Murugesan Suresh Kumar
Anish Babu
Ramachandran Murugesan
Kadarkaraithangam Jeyasubramanian
author_sort Murugesan Suresh Kumar
collection DOAJ
description The present study describes the development and evaluation of a novel biocompatible dendrimer-based nano drug delivery system which is readily soluble in water prepared by condensing Phloroglucinol and Succinic Acid (PGSA) and could efficiently encapsulate a well known hydrophobic photodynamic therapy (PDT) agent, protoporphyrin Ⅸ (PpⅨ). The (dark and photo) cytotoxicity of the PGSA-PpⅨ (dendrimer-drug) formulation towards Dalton Lymphoma Ascites (DLA) cancer cell lines upon visible light treatment is reported and evaluated the cytotoxic Reactive Oxygen Species (ROS) generation efficiency of the Protoporphyrin Ⅸ in free and dendrimer encapsulated forms. The in vitro toxicity demonstrated by PpⅨ loaded PGSA dendrimer nanoformulation, on DLA cells reveals that this novel PGSA nanocarrier reduces the toxic nature of PpⅨ when compared to free PpⅨ without light treatment which satisfy the aim of our study. On the other hand, treatment of DLA cells with PGSA dendrimer formulation in combination with light resulted in significant enhancement in therapeutic efficacy of the PDT agent, PpⅨ.
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spelling doaj.art-44df11c87918492bbe6fd19e41a3f9162023-09-26T10:12:42ZengTsinghua University PressNano Biomedicine and Engineering2150-55782012-09-014313213810.5101/nbe.v4i3.p132-138Novel water soluble dendrimer nanocarrier for enhanced photodynamic efficacy of protoporphyrin ⅨMurugesan Suresh Kumar0Anish Babu1Ramachandran Murugesan2Kadarkaraithangam Jeyasubramanian3Department of Nanoscience and Technology, Mepco Schlenk Engineering College, Sivakasi-626005, INDIASchool of Biological Sciences, Madurai Kamaraj University, Madurai-625021, INDIASchool of Biological Sciences, Madurai Kamaraj University, Madurai-625021, INDIADepartment of Nanoscience and Technology, Mepco Schlenk Engineering College, Sivakasi-626005, INDIAThe present study describes the development and evaluation of a novel biocompatible dendrimer-based nano drug delivery system which is readily soluble in water prepared by condensing Phloroglucinol and Succinic Acid (PGSA) and could efficiently encapsulate a well known hydrophobic photodynamic therapy (PDT) agent, protoporphyrin Ⅸ (PpⅨ). The (dark and photo) cytotoxicity of the PGSA-PpⅨ (dendrimer-drug) formulation towards Dalton Lymphoma Ascites (DLA) cancer cell lines upon visible light treatment is reported and evaluated the cytotoxic Reactive Oxygen Species (ROS) generation efficiency of the Protoporphyrin Ⅸ in free and dendrimer encapsulated forms. The in vitro toxicity demonstrated by PpⅨ loaded PGSA dendrimer nanoformulation, on DLA cells reveals that this novel PGSA nanocarrier reduces the toxic nature of PpⅨ when compared to free PpⅨ without light treatment which satisfy the aim of our study. On the other hand, treatment of DLA cells with PGSA dendrimer formulation in combination with light resulted in significant enhancement in therapeutic efficacy of the PDT agent, PpⅨ.https://www.sciopen.com/article/10.5101/nbe.v4i3.p132-138dendrimerphloroglucinol-succinic acidnano carrierppⅸros generationin vitro toxicityphotodynamic therapy
spellingShingle Murugesan Suresh Kumar
Anish Babu
Ramachandran Murugesan
Kadarkaraithangam Jeyasubramanian
Novel water soluble dendrimer nanocarrier for enhanced photodynamic efficacy of protoporphyrin Ⅸ
Nano Biomedicine and Engineering
dendrimer
phloroglucinol-succinic acid
nano carrier
ppⅸ
ros generation
in vitro toxicity
photodynamic therapy
title Novel water soluble dendrimer nanocarrier for enhanced photodynamic efficacy of protoporphyrin Ⅸ
title_full Novel water soluble dendrimer nanocarrier for enhanced photodynamic efficacy of protoporphyrin Ⅸ
title_fullStr Novel water soluble dendrimer nanocarrier for enhanced photodynamic efficacy of protoporphyrin Ⅸ
title_full_unstemmed Novel water soluble dendrimer nanocarrier for enhanced photodynamic efficacy of protoporphyrin Ⅸ
title_short Novel water soluble dendrimer nanocarrier for enhanced photodynamic efficacy of protoporphyrin Ⅸ
title_sort novel water soluble dendrimer nanocarrier for enhanced photodynamic efficacy of protoporphyrin ix
topic dendrimer
phloroglucinol-succinic acid
nano carrier
ppⅸ
ros generation
in vitro toxicity
photodynamic therapy
url https://www.sciopen.com/article/10.5101/nbe.v4i3.p132-138
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AT ramachandranmurugesan novelwatersolubledendrimernanocarrierforenhancedphotodynamicefficacyofprotoporphyrinix
AT kadarkaraithangamjeyasubramanian novelwatersolubledendrimernanocarrierforenhancedphotodynamicefficacyofprotoporphyrinix