Novel water soluble dendrimer nanocarrier for enhanced photodynamic efficacy of protoporphyrin Ⅸ
The present study describes the development and evaluation of a novel biocompatible dendrimer-based nano drug delivery system which is readily soluble in water prepared by condensing Phloroglucinol and Succinic Acid (PGSA) and could efficiently encapsulate a well known hydrophobic photodynamic thera...
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Format: | Article |
Language: | English |
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Tsinghua University Press
2012-09-01
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Series: | Nano Biomedicine and Engineering |
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Online Access: | https://www.sciopen.com/article/10.5101/nbe.v4i3.p132-138 |
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author | Murugesan Suresh Kumar Anish Babu Ramachandran Murugesan Kadarkaraithangam Jeyasubramanian |
author_facet | Murugesan Suresh Kumar Anish Babu Ramachandran Murugesan Kadarkaraithangam Jeyasubramanian |
author_sort | Murugesan Suresh Kumar |
collection | DOAJ |
description | The present study describes the development and evaluation of a novel biocompatible dendrimer-based nano drug delivery system which is readily soluble in water prepared by condensing Phloroglucinol and Succinic Acid (PGSA) and could efficiently encapsulate a well known hydrophobic photodynamic therapy (PDT) agent, protoporphyrin Ⅸ (PpⅨ). The (dark and photo) cytotoxicity of the PGSA-PpⅨ (dendrimer-drug) formulation towards Dalton Lymphoma Ascites (DLA) cancer cell lines upon visible light treatment is reported and evaluated the cytotoxic Reactive Oxygen Species (ROS) generation efficiency of the Protoporphyrin Ⅸ in free and dendrimer encapsulated forms. The in vitro toxicity demonstrated by PpⅨ loaded PGSA dendrimer nanoformulation, on DLA cells reveals that this novel PGSA nanocarrier reduces the toxic nature of PpⅨ when compared to free PpⅨ without light treatment which satisfy the aim of our study. On the other hand, treatment of DLA cells with PGSA dendrimer formulation in combination with light resulted in significant enhancement in therapeutic efficacy of the PDT agent, PpⅨ. |
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format | Article |
id | doaj.art-44df11c87918492bbe6fd19e41a3f916 |
institution | Directory Open Access Journal |
issn | 2150-5578 |
language | English |
last_indexed | 2024-03-11T21:45:23Z |
publishDate | 2012-09-01 |
publisher | Tsinghua University Press |
record_format | Article |
series | Nano Biomedicine and Engineering |
spelling | doaj.art-44df11c87918492bbe6fd19e41a3f9162023-09-26T10:12:42ZengTsinghua University PressNano Biomedicine and Engineering2150-55782012-09-014313213810.5101/nbe.v4i3.p132-138Novel water soluble dendrimer nanocarrier for enhanced photodynamic efficacy of protoporphyrin ⅨMurugesan Suresh Kumar0Anish Babu1Ramachandran Murugesan2Kadarkaraithangam Jeyasubramanian3Department of Nanoscience and Technology, Mepco Schlenk Engineering College, Sivakasi-626005, INDIASchool of Biological Sciences, Madurai Kamaraj University, Madurai-625021, INDIASchool of Biological Sciences, Madurai Kamaraj University, Madurai-625021, INDIADepartment of Nanoscience and Technology, Mepco Schlenk Engineering College, Sivakasi-626005, INDIAThe present study describes the development and evaluation of a novel biocompatible dendrimer-based nano drug delivery system which is readily soluble in water prepared by condensing Phloroglucinol and Succinic Acid (PGSA) and could efficiently encapsulate a well known hydrophobic photodynamic therapy (PDT) agent, protoporphyrin Ⅸ (PpⅨ). The (dark and photo) cytotoxicity of the PGSA-PpⅨ (dendrimer-drug) formulation towards Dalton Lymphoma Ascites (DLA) cancer cell lines upon visible light treatment is reported and evaluated the cytotoxic Reactive Oxygen Species (ROS) generation efficiency of the Protoporphyrin Ⅸ in free and dendrimer encapsulated forms. The in vitro toxicity demonstrated by PpⅨ loaded PGSA dendrimer nanoformulation, on DLA cells reveals that this novel PGSA nanocarrier reduces the toxic nature of PpⅨ when compared to free PpⅨ without light treatment which satisfy the aim of our study. On the other hand, treatment of DLA cells with PGSA dendrimer formulation in combination with light resulted in significant enhancement in therapeutic efficacy of the PDT agent, PpⅨ.https://www.sciopen.com/article/10.5101/nbe.v4i3.p132-138dendrimerphloroglucinol-succinic acidnano carrierppⅸros generationin vitro toxicityphotodynamic therapy |
spellingShingle | Murugesan Suresh Kumar Anish Babu Ramachandran Murugesan Kadarkaraithangam Jeyasubramanian Novel water soluble dendrimer nanocarrier for enhanced photodynamic efficacy of protoporphyrin Ⅸ Nano Biomedicine and Engineering dendrimer phloroglucinol-succinic acid nano carrier ppⅸ ros generation in vitro toxicity photodynamic therapy |
title | Novel water soluble dendrimer nanocarrier for enhanced photodynamic efficacy of protoporphyrin Ⅸ |
title_full | Novel water soluble dendrimer nanocarrier for enhanced photodynamic efficacy of protoporphyrin Ⅸ |
title_fullStr | Novel water soluble dendrimer nanocarrier for enhanced photodynamic efficacy of protoporphyrin Ⅸ |
title_full_unstemmed | Novel water soluble dendrimer nanocarrier for enhanced photodynamic efficacy of protoporphyrin Ⅸ |
title_short | Novel water soluble dendrimer nanocarrier for enhanced photodynamic efficacy of protoporphyrin Ⅸ |
title_sort | novel water soluble dendrimer nanocarrier for enhanced photodynamic efficacy of protoporphyrin ix |
topic | dendrimer phloroglucinol-succinic acid nano carrier ppⅸ ros generation in vitro toxicity photodynamic therapy |
url | https://www.sciopen.com/article/10.5101/nbe.v4i3.p132-138 |
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