Superhydrophilic/Superhydrophobic Droplet Microarrays of Low Surface Tension Biofluids for Nucleic Acid Detection
Abstract Superhydrophilic/superhydrophobic patterned surfaces can be used to create droplet microarrays. A specific challenge with the liquids needed for various biomedical applications, as compared to pure water, is their lower surface tension and potential for contaminating the surfaces through ad...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Wiley-VCH
2024-01-01
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| Series: | Advanced Materials Interfaces |
| Subjects: | |
| Online Access: | https://doi.org/10.1002/admi.202300596 |
| _version_ | 1797365503290769408 |
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| author | Mohammad Awashra Pinja Elomaa Tuomas Ojalehto Päivi Saavalainen Ville Jokinen |
| author_facet | Mohammad Awashra Pinja Elomaa Tuomas Ojalehto Päivi Saavalainen Ville Jokinen |
| author_sort | Mohammad Awashra |
| collection | DOAJ |
| description | Abstract Superhydrophilic/superhydrophobic patterned surfaces can be used to create droplet microarrays. A specific challenge with the liquids needed for various biomedical applications, as compared to pure water, is their lower surface tension and potential for contaminating the surfaces through adsorption. Here, a method is shown to create biofluid droplet microarrays using discontinuous dewetting of pure water, an oil protective layer, and finally biofluid exchange with the water droplet array. With this method, a droplet array of a viscous nucleic acid amplification solution can be formed with a low surface tension of 34 mN m−1 and a contact angle of only 76° with the used hydrophobic coating. This droplet array is applied for nucleic acid detection of SARS‐CoV‐2 virus using strand invasion‐based amplification (SIBA) technology. It is shown that by using an array of 10 000 droplets of 50 µm diameter the limit of detection is 1 RNA copy µL−1. The results demonstrate that SIBA on droplet microarrays may be a quantitative technology. |
| first_indexed | 2024-03-08T16:49:54Z |
| format | Article |
| id | doaj.art-44e15b76cbd24515a16ad3d6293d491b |
| institution | Directory Open Access Journal |
| issn | 2196-7350 |
| language | English |
| last_indexed | 2024-03-08T16:49:54Z |
| publishDate | 2024-01-01 |
| publisher | Wiley-VCH |
| record_format | Article |
| series | Advanced Materials Interfaces |
| spelling | doaj.art-44e15b76cbd24515a16ad3d6293d491b2024-01-05T04:51:01ZengWiley-VCHAdvanced Materials Interfaces2196-73502024-01-01111n/an/a10.1002/admi.202300596Superhydrophilic/Superhydrophobic Droplet Microarrays of Low Surface Tension Biofluids for Nucleic Acid DetectionMohammad Awashra0Pinja Elomaa1Tuomas Ojalehto2Päivi Saavalainen3Ville Jokinen4Department of Chemistry and Materials Science School of Chemical Engineering Aalto University Tietotie 3 Espoo 02150 FinlandTranslational Immunology Research Program Folkhälsan Research Center University of Helsinki Haartmaninkatu 8 Helsinki 00290 FinlandAidian Oy Koivu‐Mankkaan tie 6 B Espoo 02200 FinlandTranslational Immunology Research Program Folkhälsan Research Center University of Helsinki Haartmaninkatu 8 Helsinki 00290 FinlandDepartment of Chemistry and Materials Science School of Chemical Engineering Aalto University Tietotie 3 Espoo 02150 FinlandAbstract Superhydrophilic/superhydrophobic patterned surfaces can be used to create droplet microarrays. A specific challenge with the liquids needed for various biomedical applications, as compared to pure water, is their lower surface tension and potential for contaminating the surfaces through adsorption. Here, a method is shown to create biofluid droplet microarrays using discontinuous dewetting of pure water, an oil protective layer, and finally biofluid exchange with the water droplet array. With this method, a droplet array of a viscous nucleic acid amplification solution can be formed with a low surface tension of 34 mN m−1 and a contact angle of only 76° with the used hydrophobic coating. This droplet array is applied for nucleic acid detection of SARS‐CoV‐2 virus using strand invasion‐based amplification (SIBA) technology. It is shown that by using an array of 10 000 droplets of 50 µm diameter the limit of detection is 1 RNA copy µL−1. The results demonstrate that SIBA on droplet microarrays may be a quantitative technology.https://doi.org/10.1002/admi.202300596biofluidsblack silicondigital nucleic acid amplificationmicrofluidicswettability patterning |
| spellingShingle | Mohammad Awashra Pinja Elomaa Tuomas Ojalehto Päivi Saavalainen Ville Jokinen Superhydrophilic/Superhydrophobic Droplet Microarrays of Low Surface Tension Biofluids for Nucleic Acid Detection Advanced Materials Interfaces biofluids black silicon digital nucleic acid amplification microfluidics wettability patterning |
| title | Superhydrophilic/Superhydrophobic Droplet Microarrays of Low Surface Tension Biofluids for Nucleic Acid Detection |
| title_full | Superhydrophilic/Superhydrophobic Droplet Microarrays of Low Surface Tension Biofluids for Nucleic Acid Detection |
| title_fullStr | Superhydrophilic/Superhydrophobic Droplet Microarrays of Low Surface Tension Biofluids for Nucleic Acid Detection |
| title_full_unstemmed | Superhydrophilic/Superhydrophobic Droplet Microarrays of Low Surface Tension Biofluids for Nucleic Acid Detection |
| title_short | Superhydrophilic/Superhydrophobic Droplet Microarrays of Low Surface Tension Biofluids for Nucleic Acid Detection |
| title_sort | superhydrophilic superhydrophobic droplet microarrays of low surface tension biofluids for nucleic acid detection |
| topic | biofluids black silicon digital nucleic acid amplification microfluidics wettability patterning |
| url | https://doi.org/10.1002/admi.202300596 |
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