Transcriptional Profiling of Homologous Recombination Pathway Genes in <i>Mycobacterium bovis</i> BCG Moreau
<i>Mycobacterium bovis</i> BCG Moreau is the main Brazilian strain for vaccination against tuberculosis. It is considered an early strain, more like the original BCG, whereas BCG Pasteur, largely used as a reference, belongs to the late strain clade. BCG Moreau, contrary to Pasteur, is n...
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| Format: | Article |
| Language: | English |
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MDPI AG
2023-10-01
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| Series: | Microorganisms |
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| Online Access: | https://www.mdpi.com/2076-2607/11/10/2534 |
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| author | Marcos Gustavo Araujo Schwarz Paloma Rezende Corrêa Leila Mendonça-Lima |
| author_facet | Marcos Gustavo Araujo Schwarz Paloma Rezende Corrêa Leila Mendonça-Lima |
| author_sort | Marcos Gustavo Araujo Schwarz |
| collection | DOAJ |
| description | <i>Mycobacterium bovis</i> BCG Moreau is the main Brazilian strain for vaccination against tuberculosis. It is considered an early strain, more like the original BCG, whereas BCG Pasteur, largely used as a reference, belongs to the late strain clade. BCG Moreau, contrary to Pasteur, is naturally deficient in homologous recombination (HR). In this work, using a UV exposure test, we aimed to detect differences in the survival of various BCG strains after DNA damage. Transcription of core and regulatory HR genes was further analyzed using RT-qPCR, aiming to identify the molecular agent responsible for this phenotype. We show that early strains share the Moreau low survival rate after UV exposure, whereas late strains mimic the Pasteur phenotype, indicating that this increase in HR efficiency is linked to the evolutionary clade history. Additionally, RT-qPCR shows that BCG Moreau has an overall lower level of these transcripts than Pasteur, indicating a correlation between this gene expression profile and HR efficiency. Further assays should be performed to fully identify the molecular mechanism that may explain this differential phenotype between early and late BCG strains. |
| first_indexed | 2024-03-10T21:01:28Z |
| format | Article |
| id | doaj.art-44e34c0e09cd407eb60e088de2a34628 |
| institution | Directory Open Access Journal |
| issn | 2076-2607 |
| language | English |
| last_indexed | 2024-03-10T21:01:28Z |
| publishDate | 2023-10-01 |
| publisher | MDPI AG |
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| series | Microorganisms |
| spelling | doaj.art-44e34c0e09cd407eb60e088de2a346282023-11-19T17:28:13ZengMDPI AGMicroorganisms2076-26072023-10-011110253410.3390/microorganisms11102534Transcriptional Profiling of Homologous Recombination Pathway Genes in <i>Mycobacterium bovis</i> BCG MoreauMarcos Gustavo Araujo Schwarz0Paloma Rezende Corrêa1Leila Mendonça-Lima2Laboratório de Biologia Molecular Aplicada à Micobactérias, Instituto Oswaldo Cruz, Fiocruz, Rio de Janeiro 21040-900, RJ, BrazilLaboratório de Biologia Molecular Aplicada à Micobactérias, Instituto Oswaldo Cruz, Fiocruz, Rio de Janeiro 21040-900, RJ, BrazilLaboratório de Biologia Molecular Aplicada à Micobactérias, Instituto Oswaldo Cruz, Fiocruz, Rio de Janeiro 21040-900, RJ, Brazil<i>Mycobacterium bovis</i> BCG Moreau is the main Brazilian strain for vaccination against tuberculosis. It is considered an early strain, more like the original BCG, whereas BCG Pasteur, largely used as a reference, belongs to the late strain clade. BCG Moreau, contrary to Pasteur, is naturally deficient in homologous recombination (HR). In this work, using a UV exposure test, we aimed to detect differences in the survival of various BCG strains after DNA damage. Transcription of core and regulatory HR genes was further analyzed using RT-qPCR, aiming to identify the molecular agent responsible for this phenotype. We show that early strains share the Moreau low survival rate after UV exposure, whereas late strains mimic the Pasteur phenotype, indicating that this increase in HR efficiency is linked to the evolutionary clade history. Additionally, RT-qPCR shows that BCG Moreau has an overall lower level of these transcripts than Pasteur, indicating a correlation between this gene expression profile and HR efficiency. Further assays should be performed to fully identify the molecular mechanism that may explain this differential phenotype between early and late BCG strains.https://www.mdpi.com/2076-2607/11/10/2534homologous recombination<i>Mycobacterium bovis</i> BCGDNA damage response pathwaysultraviolet radiation exposure survival |
| spellingShingle | Marcos Gustavo Araujo Schwarz Paloma Rezende Corrêa Leila Mendonça-Lima Transcriptional Profiling of Homologous Recombination Pathway Genes in <i>Mycobacterium bovis</i> BCG Moreau Microorganisms homologous recombination <i>Mycobacterium bovis</i> BCG DNA damage response pathways ultraviolet radiation exposure survival |
| title | Transcriptional Profiling of Homologous Recombination Pathway Genes in <i>Mycobacterium bovis</i> BCG Moreau |
| title_full | Transcriptional Profiling of Homologous Recombination Pathway Genes in <i>Mycobacterium bovis</i> BCG Moreau |
| title_fullStr | Transcriptional Profiling of Homologous Recombination Pathway Genes in <i>Mycobacterium bovis</i> BCG Moreau |
| title_full_unstemmed | Transcriptional Profiling of Homologous Recombination Pathway Genes in <i>Mycobacterium bovis</i> BCG Moreau |
| title_short | Transcriptional Profiling of Homologous Recombination Pathway Genes in <i>Mycobacterium bovis</i> BCG Moreau |
| title_sort | transcriptional profiling of homologous recombination pathway genes in i mycobacterium bovis i bcg moreau |
| topic | homologous recombination <i>Mycobacterium bovis</i> BCG DNA damage response pathways ultraviolet radiation exposure survival |
| url | https://www.mdpi.com/2076-2607/11/10/2534 |
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