Loss of bcbrn1 and bcpks13 in Botrytis cinerea Not Only Blocks Melanization But Also Increases Vegetative Growth and Virulence
Botrytis cinerea is a necrotrophic pathogen that causes gray mold disease in a broad range of plants. Dihydroxynaphthalene (DHN) melanin is a major component of the extracellular matrix of B. cinerea, but knowledge of the exact role of melanin biosynthesis in this pathogen is unclear. In this study,...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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The American Phytopathological Society
2015-10-01
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| Series: | Molecular Plant-Microbe Interactions |
| Online Access: | https://apsjournals.apsnet.org/doi/10.1094/MPMI-04-15-0085-R |
| _version_ | 1811287289063538688 |
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| author | Chenghua Zhang Yifan He Pinkuan Zhu Lu Chen Yiwen Wang Bing Ni Ling Xu |
| author_facet | Chenghua Zhang Yifan He Pinkuan Zhu Lu Chen Yiwen Wang Bing Ni Ling Xu |
| author_sort | Chenghua Zhang |
| collection | DOAJ |
| description | Botrytis cinerea is a necrotrophic pathogen that causes gray mold disease in a broad range of plants. Dihydroxynaphthalene (DHN) melanin is a major component of the extracellular matrix of B. cinerea, but knowledge of the exact role of melanin biosynthesis in this pathogen is unclear. In this study, we characterize two genes in B. cinerea, bcpks13 and bcbrn1, encoding polyketide synthase and tetrahydroxynaphthalene (THN) reductases, respectively, and both have predicted roles in DHN melanin biosynthesis. The ∆bcpks13 and ∆bcbrn1 mutants show white and orange pigmentation, respectively, and the mutants are also deficient in conidiation in vitro but show enhanced growth rates and virulence on hosts. Moreover, the mutants display elevated acidification of the complete medium (CM), probably due to oxalic acid secretion and secretion of cell wall–degrading enzymes, and preferably utilize plant cell-wall components as carbon sources for mycelium growth in vitro. In contrast, overexpression of bcbrn1 (OE::bcbrn1 strain) results in attenuated hydrolytic enzyme secretion, acidification ability, and virulence. Taken together, these results indicate that bcpks13 and bcbrn1 participate in diverse cellular and developmental processes, such as melanization and conidiation in B. cinerea in vitro, but they negatively regulate the virulence of this pathogen. |
| first_indexed | 2024-04-13T03:16:18Z |
| format | Article |
| id | doaj.art-44e50085c59d4e60842db269353a5862 |
| institution | Directory Open Access Journal |
| issn | 0894-0282 1943-7706 |
| language | English |
| last_indexed | 2024-04-13T03:16:18Z |
| publishDate | 2015-10-01 |
| publisher | The American Phytopathological Society |
| record_format | Article |
| series | Molecular Plant-Microbe Interactions |
| spelling | doaj.art-44e50085c59d4e60842db269353a58622022-12-22T03:04:54ZengThe American Phytopathological SocietyMolecular Plant-Microbe Interactions0894-02821943-77062015-10-0128101091110110.1094/MPMI-04-15-0085-RLoss of bcbrn1 and bcpks13 in Botrytis cinerea Not Only Blocks Melanization But Also Increases Vegetative Growth and VirulenceChenghua Zhang0Yifan He1Pinkuan Zhu2Lu Chen3Yiwen Wang4Bing Ni5Ling Xu6School of Life Science, East China Normal University, Shanghai 200241, PR ChinaSchool of Life Science, East China Normal University, Shanghai 200241, PR ChinaSchool of Life Science, East China Normal University, Shanghai 200241, PR ChinaSchool of Life Science, East China Normal University, Shanghai 200241, PR ChinaSchool of Life Science, East China Normal University, Shanghai 200241, PR ChinaSchool of Life Science, East China Normal University, Shanghai 200241, PR ChinaSchool of Life Science, East China Normal University, Shanghai 200241, PR ChinaBotrytis cinerea is a necrotrophic pathogen that causes gray mold disease in a broad range of plants. Dihydroxynaphthalene (DHN) melanin is a major component of the extracellular matrix of B. cinerea, but knowledge of the exact role of melanin biosynthesis in this pathogen is unclear. In this study, we characterize two genes in B. cinerea, bcpks13 and bcbrn1, encoding polyketide synthase and tetrahydroxynaphthalene (THN) reductases, respectively, and both have predicted roles in DHN melanin biosynthesis. The ∆bcpks13 and ∆bcbrn1 mutants show white and orange pigmentation, respectively, and the mutants are also deficient in conidiation in vitro but show enhanced growth rates and virulence on hosts. Moreover, the mutants display elevated acidification of the complete medium (CM), probably due to oxalic acid secretion and secretion of cell wall–degrading enzymes, and preferably utilize plant cell-wall components as carbon sources for mycelium growth in vitro. In contrast, overexpression of bcbrn1 (OE::bcbrn1 strain) results in attenuated hydrolytic enzyme secretion, acidification ability, and virulence. Taken together, these results indicate that bcpks13 and bcbrn1 participate in diverse cellular and developmental processes, such as melanization and conidiation in B. cinerea in vitro, but they negatively regulate the virulence of this pathogen.https://apsjournals.apsnet.org/doi/10.1094/MPMI-04-15-0085-R |
| spellingShingle | Chenghua Zhang Yifan He Pinkuan Zhu Lu Chen Yiwen Wang Bing Ni Ling Xu Loss of bcbrn1 and bcpks13 in Botrytis cinerea Not Only Blocks Melanization But Also Increases Vegetative Growth and Virulence Molecular Plant-Microbe Interactions |
| title | Loss of bcbrn1 and bcpks13 in Botrytis cinerea Not Only Blocks Melanization But Also Increases Vegetative Growth and Virulence |
| title_full | Loss of bcbrn1 and bcpks13 in Botrytis cinerea Not Only Blocks Melanization But Also Increases Vegetative Growth and Virulence |
| title_fullStr | Loss of bcbrn1 and bcpks13 in Botrytis cinerea Not Only Blocks Melanization But Also Increases Vegetative Growth and Virulence |
| title_full_unstemmed | Loss of bcbrn1 and bcpks13 in Botrytis cinerea Not Only Blocks Melanization But Also Increases Vegetative Growth and Virulence |
| title_short | Loss of bcbrn1 and bcpks13 in Botrytis cinerea Not Only Blocks Melanization But Also Increases Vegetative Growth and Virulence |
| title_sort | loss of bcbrn1 and bcpks13 in botrytis cinerea not only blocks melanization but also increases vegetative growth and virulence |
| url | https://apsjournals.apsnet.org/doi/10.1094/MPMI-04-15-0085-R |
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