Indole produced during dysbiosis mediates host–microorganism chemical communication
An imbalance of the gut microbiota, termed dysbiosis, has a substantial impact on host physiology. However, the mechanism by which host deals with gut dysbiosis to maintain fitness remains largely unknown. In Caenorhabditis elegans, Escherichia coli, which is its bacterial diet, proliferates in its...
| Main Authors: | , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
eLife Sciences Publications Ltd
2023-11-01
|
| Series: | eLife |
| Subjects: | |
| Online Access: | https://elifesciences.org/articles/85362 |
| _version_ | 1797434894628945920 |
|---|---|
| author | Rui-Qiu Yang Yong-Hong Chen Qin-yi Wu Jie Tang Shan-Zhuang Niu Qiu Zhao Yi-Cheng Ma Cheng-Gang Zou |
| author_facet | Rui-Qiu Yang Yong-Hong Chen Qin-yi Wu Jie Tang Shan-Zhuang Niu Qiu Zhao Yi-Cheng Ma Cheng-Gang Zou |
| author_sort | Rui-Qiu Yang |
| collection | DOAJ |
| description | An imbalance of the gut microbiota, termed dysbiosis, has a substantial impact on host physiology. However, the mechanism by which host deals with gut dysbiosis to maintain fitness remains largely unknown. In Caenorhabditis elegans, Escherichia coli, which is its bacterial diet, proliferates in its intestinal lumen during aging. Here, we demonstrate that progressive intestinal proliferation of E. coli activates the transcription factor DAF-16, which is required for maintenance of longevity and organismal fitness in worms with age. DAF-16 up-regulates two lysozymes lys-7 and lys-8, thus limiting the bacterial accumulation in the gut of worms during aging. During dysbiosis, the levels of indole produced by E. coli are increased in worms. Indole is involved in the activation of DAF-16 by TRPA-1 in neurons of worms. Our finding demonstrates that indole functions as a microbial signal of gut dysbiosis to promote fitness of the host. |
| first_indexed | 2024-03-09T10:39:30Z |
| format | Article |
| id | doaj.art-44e77e10611f4d059fb5bee44e43b96c |
| institution | Directory Open Access Journal |
| issn | 2050-084X |
| language | English |
| last_indexed | 2024-03-09T10:39:30Z |
| publishDate | 2023-11-01 |
| publisher | eLife Sciences Publications Ltd |
| record_format | Article |
| series | eLife |
| spelling | doaj.art-44e77e10611f4d059fb5bee44e43b96c2023-12-01T15:47:01ZengeLife Sciences Publications LtdeLife2050-084X2023-11-011210.7554/eLife.85362Indole produced during dysbiosis mediates host–microorganism chemical communicationRui-Qiu Yang0Yong-Hong Chen1Qin-yi Wu2Jie Tang3Shan-Zhuang Niu4Qiu Zhao5Yi-Cheng Ma6Cheng-Gang Zou7https://orcid.org/0000-0001-5519-4402State key Laboratory for Conservation and Utilization of Bio-Resources in Yunnan, Yunnan University, Kunming, ChinaState key Laboratory for Conservation and Utilization of Bio-Resources in Yunnan, Yunnan University, Kunming, ChinaYunnan Provincial Key Laboratory of Molecular Biology for Sinomedicine, Yunnan University of Traditional Chinese Medicine, Kunming, ChinaState key Laboratory for Conservation and Utilization of Bio-Resources in Yunnan, Yunnan University, Kunming, China; Yunnan Key Laboratory of Vaccine Research Development on Severe Infectious Disease, Chinese Academy of Medical Sciences and Peking Union Medical College, Kunming, ChinaState key Laboratory for Conservation and Utilization of Bio-Resources in Yunnan, Yunnan University, Kunming, ChinaState key Laboratory for Conservation and Utilization of Bio-Resources in Yunnan, Yunnan University, Kunming, ChinaState key Laboratory for Conservation and Utilization of Bio-Resources in Yunnan, Yunnan University, Kunming, ChinaState key Laboratory for Conservation and Utilization of Bio-Resources in Yunnan, Yunnan University, Kunming, ChinaAn imbalance of the gut microbiota, termed dysbiosis, has a substantial impact on host physiology. However, the mechanism by which host deals with gut dysbiosis to maintain fitness remains largely unknown. In Caenorhabditis elegans, Escherichia coli, which is its bacterial diet, proliferates in its intestinal lumen during aging. Here, we demonstrate that progressive intestinal proliferation of E. coli activates the transcription factor DAF-16, which is required for maintenance of longevity and organismal fitness in worms with age. DAF-16 up-regulates two lysozymes lys-7 and lys-8, thus limiting the bacterial accumulation in the gut of worms during aging. During dysbiosis, the levels of indole produced by E. coli are increased in worms. Indole is involved in the activation of DAF-16 by TRPA-1 in neurons of worms. Our finding demonstrates that indole functions as a microbial signal of gut dysbiosis to promote fitness of the host.https://elifesciences.org/articles/85362C. elegansE. coliindoleDAF-16TRPA-1gut dysbiosis |
| spellingShingle | Rui-Qiu Yang Yong-Hong Chen Qin-yi Wu Jie Tang Shan-Zhuang Niu Qiu Zhao Yi-Cheng Ma Cheng-Gang Zou Indole produced during dysbiosis mediates host–microorganism chemical communication eLife C. elegans E. coli indole DAF-16 TRPA-1 gut dysbiosis |
| title | Indole produced during dysbiosis mediates host–microorganism chemical communication |
| title_full | Indole produced during dysbiosis mediates host–microorganism chemical communication |
| title_fullStr | Indole produced during dysbiosis mediates host–microorganism chemical communication |
| title_full_unstemmed | Indole produced during dysbiosis mediates host–microorganism chemical communication |
| title_short | Indole produced during dysbiosis mediates host–microorganism chemical communication |
| title_sort | indole produced during dysbiosis mediates host microorganism chemical communication |
| topic | C. elegans E. coli indole DAF-16 TRPA-1 gut dysbiosis |
| url | https://elifesciences.org/articles/85362 |
| work_keys_str_mv | AT ruiqiuyang indoleproducedduringdysbiosismediateshostmicroorganismchemicalcommunication AT yonghongchen indoleproducedduringdysbiosismediateshostmicroorganismchemicalcommunication AT qinyiwu indoleproducedduringdysbiosismediateshostmicroorganismchemicalcommunication AT jietang indoleproducedduringdysbiosismediateshostmicroorganismchemicalcommunication AT shanzhuangniu indoleproducedduringdysbiosismediateshostmicroorganismchemicalcommunication AT qiuzhao indoleproducedduringdysbiosismediateshostmicroorganismchemicalcommunication AT yichengma indoleproducedduringdysbiosismediateshostmicroorganismchemicalcommunication AT chenggangzou indoleproducedduringdysbiosismediateshostmicroorganismchemicalcommunication |