The role of oxidative stress in streptozotocin-induced diabetic nephropathy in rats
ABSTRACT Objective The objective of this study was to evaluate the role of oxidative stress in an experimental model of streptozotocin-induced diabetic nephropathy in rats. Materials and methods Wistar, adult, male rats were used in the study. Animals were divided in the following groups: Citrat...
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| Format: | Article |
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Brazilian Society of Endocrinology and Metabolism
2016-08-01
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| Series: | Archives of Endocrinology and Metabolism |
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| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S2359-39972016000500443&lng=en&tlng=en |
| _version_ | 1811213820116336640 |
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| author | Sheila Marques Fernandes Priscilla Mendes Cordeiro Mirian Watanabe Cassiane Dezoti da Fonseca Maria de Fatima Fernandes Vattimo |
| author_facet | Sheila Marques Fernandes Priscilla Mendes Cordeiro Mirian Watanabe Cassiane Dezoti da Fonseca Maria de Fatima Fernandes Vattimo |
| author_sort | Sheila Marques Fernandes |
| collection | DOAJ |
| description | ABSTRACT Objective The objective of this study was to evaluate the role of oxidative stress in an experimental model of streptozotocin-induced diabetic nephropathy in rats. Materials and methods Wistar, adult, male rats were used in the study. Animals were divided in the following groups: Citrate (control, citrate buffer 0.01M, pH 4.2 was administrated intravenously - i.v - in the caudal vein), Uninephrectomy+Citrate (left uninephrectomy-20 days before the study), DM (streptozotocin, 65 mg/kg, i.v, on the 20th day of the study), Uninephrectomy+DM. Physiological parameters (water and food intake, body weight, blood glucose, kidney weight, and relative kidney weight); renal function (creatinine clearance), urine albumin (immunodiffusion method); oxidative metabolites (urinary peroxides, thiobarbituric acid reactive substances, and thiols in renal tissue), and kidney histology were evaluated. Results Polyphagia, polydipsia, hyperglycemia, and reduced body weight were observed in diabetic rats. Renal function was reduced in diabetic groups (creatinine clearance, p < 0.05). Uninephrectomy potentiated urine albumin and increased kidney weight and relative kidney weight in diabetic animals (p < 0.05). Urinary peroxides and thiobarbituric acid reactive substances were increased, and the reduction in thiol levels demonstrated endogenous substrate consumption in diabetic groups (p < 0.05). The histological analysis revealed moderate lesions of diabetic nephropathy. Conclusion This study confirms lipid peroxidation and intense consumption of the antioxidant defense system in diabetic rats. The association of hyperglycemia and uninephrectomy resulted in additional renal injury, demonstrating that the model is adequate for the study of diabetic nephropathy. |
| first_indexed | 2024-04-12T05:52:39Z |
| format | Article |
| id | doaj.art-44e9c05d42d141cf9268845083c4b99a |
| institution | Directory Open Access Journal |
| issn | 2359-4292 |
| language | English |
| last_indexed | 2024-04-12T05:52:39Z |
| publishDate | 2016-08-01 |
| publisher | Brazilian Society of Endocrinology and Metabolism |
| record_format | Article |
| series | Archives of Endocrinology and Metabolism |
| spelling | doaj.art-44e9c05d42d141cf9268845083c4b99a2022-12-22T03:45:15ZengBrazilian Society of Endocrinology and MetabolismArchives of Endocrinology and Metabolism2359-42922016-08-0160544344910.1590/2359-3997000000188S2359-39972016000500443The role of oxidative stress in streptozotocin-induced diabetic nephropathy in ratsSheila Marques FernandesPriscilla Mendes CordeiroMirian WatanabeCassiane Dezoti da FonsecaMaria de Fatima Fernandes VattimoABSTRACT Objective The objective of this study was to evaluate the role of oxidative stress in an experimental model of streptozotocin-induced diabetic nephropathy in rats. Materials and methods Wistar, adult, male rats were used in the study. Animals were divided in the following groups: Citrate (control, citrate buffer 0.01M, pH 4.2 was administrated intravenously - i.v - in the caudal vein), Uninephrectomy+Citrate (left uninephrectomy-20 days before the study), DM (streptozotocin, 65 mg/kg, i.v, on the 20th day of the study), Uninephrectomy+DM. Physiological parameters (water and food intake, body weight, blood glucose, kidney weight, and relative kidney weight); renal function (creatinine clearance), urine albumin (immunodiffusion method); oxidative metabolites (urinary peroxides, thiobarbituric acid reactive substances, and thiols in renal tissue), and kidney histology were evaluated. Results Polyphagia, polydipsia, hyperglycemia, and reduced body weight were observed in diabetic rats. Renal function was reduced in diabetic groups (creatinine clearance, p < 0.05). Uninephrectomy potentiated urine albumin and increased kidney weight and relative kidney weight in diabetic animals (p < 0.05). Urinary peroxides and thiobarbituric acid reactive substances were increased, and the reduction in thiol levels demonstrated endogenous substrate consumption in diabetic groups (p < 0.05). The histological analysis revealed moderate lesions of diabetic nephropathy. Conclusion This study confirms lipid peroxidation and intense consumption of the antioxidant defense system in diabetic rats. The association of hyperglycemia and uninephrectomy resulted in additional renal injury, demonstrating that the model is adequate for the study of diabetic nephropathy.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S2359-39972016000500443&lng=en&tlng=enOxidative stressratsdiabetic nephropathyuninephrectomy |
| spellingShingle | Sheila Marques Fernandes Priscilla Mendes Cordeiro Mirian Watanabe Cassiane Dezoti da Fonseca Maria de Fatima Fernandes Vattimo The role of oxidative stress in streptozotocin-induced diabetic nephropathy in rats Archives of Endocrinology and Metabolism Oxidative stress rats diabetic nephropathy uninephrectomy |
| title | The role of oxidative stress in streptozotocin-induced diabetic nephropathy in rats |
| title_full | The role of oxidative stress in streptozotocin-induced diabetic nephropathy in rats |
| title_fullStr | The role of oxidative stress in streptozotocin-induced diabetic nephropathy in rats |
| title_full_unstemmed | The role of oxidative stress in streptozotocin-induced diabetic nephropathy in rats |
| title_short | The role of oxidative stress in streptozotocin-induced diabetic nephropathy in rats |
| title_sort | role of oxidative stress in streptozotocin induced diabetic nephropathy in rats |
| topic | Oxidative stress rats diabetic nephropathy uninephrectomy |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S2359-39972016000500443&lng=en&tlng=en |
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