Assessment of genetic diversity in Iraqi camel breeds using Cytochrome b
Joudi breed were collected from Basrah, Al-Muthanna, Najaf, Babylon, and Wasit (10 samples each). As well as 15 samples of the Khawar breed from Anbar and 15 samples of the Hurra breed from DhiQar. The analysis of molecular variance (AMOVA) was employed This study aimed to investigate the genetic di...
| Main Authors: | , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Mirror of Research in Veterinary Sciences and Animals (MRVSA)
2019-07-01
|
| Series: | Mirror of Research in Veterinary Sciences and Animals |
| Subjects: | |
| Online Access: | http://mrvsa.com/upload/3%20Assessment%20of%20genetic%20diversity%20in%20Iraqi%20camel%20breeds%20using%20Cytochrome%20b.pdf |
| _version_ | 1819181892970217472 |
|---|---|
| author | Asaad Y. Ayied Adnan I. Al-Badran Ayoob R. Al-Zaalan |
| author_facet | Asaad Y. Ayied Adnan I. Al-Badran Ayoob R. Al-Zaalan |
| author_sort | Asaad Y. Ayied |
| collection | DOAJ |
| description | Joudi breed were collected from Basrah, Al-Muthanna, Najaf, Babylon, and Wasit (10 samples each). As well as 15 samples of the Khawar breed from Anbar and 15 samples of the Hurra breed from DhiQar. The analysis of molecular variance (AMOVA) was employed This study aimed to investigate the genetic diversity, population structure, and demographic history of Iraq camel breeds. Eighty blood samples were randomly collected from unrelated animals from different parts of Iraq. Fifty samples of the Joudi breed were collected from Basrah, Al-Muthanna, Najaf, Babylon, and Wasit (10 samples each). As well as 15 samples of the Khawar breed from Anbar and 15 samples of the Hurra breed from DhiQar. The analysis of molecular variance (AMOVA) was employed in order to determine the genetic variability within and among populations of this camel breeds. The neutrality tests and mismatch distribution analysis were also applied to assess the neutrality and demographic expansion of the populations, respectively. The results revealed a total of 16 different haplotypes with high haplotype diversity (0.648) and low-nucleotide diversity (0.00109). The haplotypes of the Cytb gene were 15 haplotypes, 10 of which were in the Joudi breed, 6 in the Hurra breed and one haplotype in the Khwar breed. The haplotype H-2 was common to the three breeds. The variations within and among populations accounted for 94.36 and 5.64% of the total variation, respectively. The results of the neutrality test for Cytb showed that the Joudi and Hurra breeds had negative values for both Tajima's D and Fu's Fs. The highest values were -1.00737 and -1.98591 for the Hurra breed and the lowest values were for the Joudi breed (-2.14737 and -6.59079 respectively). In the Khwar breed, the values were zero for Tajima's D and Fu's Fs and the obtained result conforms to the model of population expansion (t > 0 and θ1> θ0) for Joudi and Hurra breeds. |
| first_indexed | 2024-12-22T22:37:28Z |
| format | Article |
| id | doaj.art-44eca9af1b5a4f588bec1f7278cc32a5 |
| institution | Directory Open Access Journal |
| issn | 2520-324X 2520-324X |
| language | English |
| last_indexed | 2024-12-22T22:37:28Z |
| publishDate | 2019-07-01 |
| publisher | Mirror of Research in Veterinary Sciences and Animals (MRVSA) |
| record_format | Article |
| series | Mirror of Research in Veterinary Sciences and Animals |
| spelling | doaj.art-44eca9af1b5a4f588bec1f7278cc32a52022-12-21T18:10:17ZengMirror of Research in Veterinary Sciences and Animals (MRVSA)Mirror of Research in Veterinary Sciences and Animals2520-324X2520-324X2019-07-01722027Assessment of genetic diversity in Iraqi camel breeds using Cytochrome bAsaad Y. Ayied0 Adnan I. Al-Badran 1Ayoob R. Al-Zaalan2Animal Production Department, College of Agriculture, 2 Biology Department, College of Science, University of Basrah, Basrah, IraqAnimal Production Department, College of Agriculture, 2 Biology Department, College of Science, University of Basrah, Basrah, IraqAnimal Production Department, College of Agriculture, 2 Biology Department, College of Science, University of Basrah, Basrah, IraqJoudi breed were collected from Basrah, Al-Muthanna, Najaf, Babylon, and Wasit (10 samples each). As well as 15 samples of the Khawar breed from Anbar and 15 samples of the Hurra breed from DhiQar. The analysis of molecular variance (AMOVA) was employed This study aimed to investigate the genetic diversity, population structure, and demographic history of Iraq camel breeds. Eighty blood samples were randomly collected from unrelated animals from different parts of Iraq. Fifty samples of the Joudi breed were collected from Basrah, Al-Muthanna, Najaf, Babylon, and Wasit (10 samples each). As well as 15 samples of the Khawar breed from Anbar and 15 samples of the Hurra breed from DhiQar. The analysis of molecular variance (AMOVA) was employed in order to determine the genetic variability within and among populations of this camel breeds. The neutrality tests and mismatch distribution analysis were also applied to assess the neutrality and demographic expansion of the populations, respectively. The results revealed a total of 16 different haplotypes with high haplotype diversity (0.648) and low-nucleotide diversity (0.00109). The haplotypes of the Cytb gene were 15 haplotypes, 10 of which were in the Joudi breed, 6 in the Hurra breed and one haplotype in the Khwar breed. The haplotype H-2 was common to the three breeds. The variations within and among populations accounted for 94.36 and 5.64% of the total variation, respectively. The results of the neutrality test for Cytb showed that the Joudi and Hurra breeds had negative values for both Tajima's D and Fu's Fs. The highest values were -1.00737 and -1.98591 for the Hurra breed and the lowest values were for the Joudi breed (-2.14737 and -6.59079 respectively). In the Khwar breed, the values were zero for Tajima's D and Fu's Fs and the obtained result conforms to the model of population expansion (t > 0 and θ1> θ0) for Joudi and Hurra breeds.http://mrvsa.com/upload/3%20Assessment%20of%20genetic%20diversity%20in%20Iraqi%20camel%20breeds%20using%20Cytochrome%20b.pdfDromedary camel; Iraq camel breed; Cytochrome b gene (Cytb); Haplotype; Phylogenetic tree. |
| spellingShingle | Asaad Y. Ayied Adnan I. Al-Badran Ayoob R. Al-Zaalan Assessment of genetic diversity in Iraqi camel breeds using Cytochrome b Mirror of Research in Veterinary Sciences and Animals Dromedary camel; Iraq camel breed; Cytochrome b gene (Cytb); Haplotype; Phylogenetic tree. |
| title | Assessment of genetic diversity in Iraqi camel breeds using Cytochrome b |
| title_full | Assessment of genetic diversity in Iraqi camel breeds using Cytochrome b |
| title_fullStr | Assessment of genetic diversity in Iraqi camel breeds using Cytochrome b |
| title_full_unstemmed | Assessment of genetic diversity in Iraqi camel breeds using Cytochrome b |
| title_short | Assessment of genetic diversity in Iraqi camel breeds using Cytochrome b |
| title_sort | assessment of genetic diversity in iraqi camel breeds using cytochrome b |
| topic | Dromedary camel; Iraq camel breed; Cytochrome b gene (Cytb); Haplotype; Phylogenetic tree. |
| url | http://mrvsa.com/upload/3%20Assessment%20of%20genetic%20diversity%20in%20Iraqi%20camel%20breeds%20using%20Cytochrome%20b.pdf |
| work_keys_str_mv | AT asaadyayied assessmentofgeneticdiversityiniraqicamelbreedsusingcytochromeb AT adnanialbadran assessmentofgeneticdiversityiniraqicamelbreedsusingcytochromeb AT ayoobralzaalan assessmentofgeneticdiversityiniraqicamelbreedsusingcytochromeb |