LuxR402 of Novosphingobium sp. HR1a regulates the correct configuration of cell envelopes
Although there is some evidence to suggest that LuxR-solo proteins participate in inter-species or even inter-kingdom communication, most of the LuxR-solo protein functions are unknown. We have characterized the LuxR402 regulator of Novosphingobium sp. HR1a, a bacterial strain with the ability to es...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2023-07-01
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| Series: | Frontiers in Microbiology |
| Subjects: | |
| Online Access: | https://www.frontiersin.org/articles/10.3389/fmicb.2023.1205860/full |
| _version_ | 1797771151706947584 |
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| author | Ana Segura Lázaro Molina |
| author_facet | Ana Segura Lázaro Molina |
| author_sort | Ana Segura |
| collection | DOAJ |
| description | Although there is some evidence to suggest that LuxR-solo proteins participate in inter-species or even inter-kingdom communication, most of the LuxR-solo protein functions are unknown. We have characterized the LuxR402 regulator of Novosphingobium sp. HR1a, a bacterial strain with the ability to establish high numbers in the plant rhizosphere and able to degrade a wide range of polycyclic aromatic hydrocarbons. LuxR402 controls the aggregation state of the bacterial culture; cultures of a mutant strain lacking this regulator flocculate in less than 3 h without agitation. We have demonstrated that the bacterial surface of the mutant is highly hydrophobic and that the mutant cells assimilate sugars slower than the wild-type. The flocculation mechanism has been demonstrated to be involved in the survival of the strain under unfavorable conditions; the luxR402 gene is repressed and produces flocculation in the presence of salicylate, a substrate that, although being assimilated by Novosphingobium, is toxic to cells at high concentrations. The flocculation of cultures in industrial setups has mainly been achieved through the addition of chemicals; these studies open up the possibility of controlling the flocculation by regulating the level of expression of the luxR402 gene. |
| first_indexed | 2024-03-12T21:33:24Z |
| format | Article |
| id | doaj.art-44ecdfb776994f0daaa1df0e19547a23 |
| institution | Directory Open Access Journal |
| issn | 1664-302X |
| language | English |
| last_indexed | 2024-03-12T21:33:24Z |
| publishDate | 2023-07-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Microbiology |
| spelling | doaj.art-44ecdfb776994f0daaa1df0e19547a232023-07-27T14:01:16ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2023-07-011410.3389/fmicb.2023.12058601205860LuxR402 of Novosphingobium sp. HR1a regulates the correct configuration of cell envelopesAna SeguraLázaro MolinaAlthough there is some evidence to suggest that LuxR-solo proteins participate in inter-species or even inter-kingdom communication, most of the LuxR-solo protein functions are unknown. We have characterized the LuxR402 regulator of Novosphingobium sp. HR1a, a bacterial strain with the ability to establish high numbers in the plant rhizosphere and able to degrade a wide range of polycyclic aromatic hydrocarbons. LuxR402 controls the aggregation state of the bacterial culture; cultures of a mutant strain lacking this regulator flocculate in less than 3 h without agitation. We have demonstrated that the bacterial surface of the mutant is highly hydrophobic and that the mutant cells assimilate sugars slower than the wild-type. The flocculation mechanism has been demonstrated to be involved in the survival of the strain under unfavorable conditions; the luxR402 gene is repressed and produces flocculation in the presence of salicylate, a substrate that, although being assimilated by Novosphingobium, is toxic to cells at high concentrations. The flocculation of cultures in industrial setups has mainly been achieved through the addition of chemicals; these studies open up the possibility of controlling the flocculation by regulating the level of expression of the luxR402 gene.https://www.frontiersin.org/articles/10.3389/fmicb.2023.1205860/fullLuxRflocculationmembrane envelopnutrient starvationstress |
| spellingShingle | Ana Segura Lázaro Molina LuxR402 of Novosphingobium sp. HR1a regulates the correct configuration of cell envelopes Frontiers in Microbiology LuxR flocculation membrane envelop nutrient starvation stress |
| title | LuxR402 of Novosphingobium sp. HR1a regulates the correct configuration of cell envelopes |
| title_full | LuxR402 of Novosphingobium sp. HR1a regulates the correct configuration of cell envelopes |
| title_fullStr | LuxR402 of Novosphingobium sp. HR1a regulates the correct configuration of cell envelopes |
| title_full_unstemmed | LuxR402 of Novosphingobium sp. HR1a regulates the correct configuration of cell envelopes |
| title_short | LuxR402 of Novosphingobium sp. HR1a regulates the correct configuration of cell envelopes |
| title_sort | luxr402 of novosphingobium sp hr1a regulates the correct configuration of cell envelopes |
| topic | LuxR flocculation membrane envelop nutrient starvation stress |
| url | https://www.frontiersin.org/articles/10.3389/fmicb.2023.1205860/full |
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