Outbreaks of an Emerging Viral Disease Covary With Differences in the Composition of the Skin Microbiome of a Wild United Kingdom Amphibian
There is growing appreciation of the important role of commensal microbes in ensuring the normal function and health of their hosts, including determining how hosts respond to pathogens. A range of infectious diseases are threatening amphibians worldwide, and evidence is accumulating that the host-a...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2019-06-01
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| Series: | Frontiers in Microbiology |
| Subjects: | |
| Online Access: | https://www.frontiersin.org/article/10.3389/fmicb.2019.01245/full |
| _version_ | 1811214710712827904 |
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| author | Lewis J. Campbell Lewis J. Campbell Trenton W. J. Garner Kevin Hopkins Amber G. F. Griffiths Xavier A. Harrison Xavier A. Harrison |
| author_facet | Lewis J. Campbell Lewis J. Campbell Trenton W. J. Garner Kevin Hopkins Amber G. F. Griffiths Xavier A. Harrison Xavier A. Harrison |
| author_sort | Lewis J. Campbell |
| collection | DOAJ |
| description | There is growing appreciation of the important role of commensal microbes in ensuring the normal function and health of their hosts, including determining how hosts respond to pathogens. A range of infectious diseases are threatening amphibians worldwide, and evidence is accumulating that the host-associated bacteria that comprise the microbiome may be key in mediating interactions between amphibian hosts and infectious pathogens. We used 16S rRNA amplicon sequencing to quantify the skin microbial community structure of over 200 individual wild adult European common frogs (Rana temporaria), from ten populations with contrasting history of the lethal disease ranavirosis, caused by emerging viral pathogens belonging to the genus Ranavirus. All populations had similar species richness irrespective of disease history, but populations that have experienced historical outbreaks of ranavirosis have a distinct skin microbiome structure (beta diversity) when compared to sites where no outbreaks of the disease have occurred. At the individual level, neither age, body length, nor sex of the frog could predict the structure of the skin microbiota. Our data potentially support the hypothesis that variation among individuals in skin microbiome structure drive differences in susceptibility to infection and lethal outbreaks of disease. More generally, our results suggest that population-level processes are more important for driving differences in microbiome structure than variation among individuals within populations in key life history traits such as age and body size. |
| first_indexed | 2024-04-12T06:09:15Z |
| format | Article |
| id | doaj.art-44ee9992716941f9a9f2092865afaea8 |
| institution | Directory Open Access Journal |
| issn | 1664-302X |
| language | English |
| last_indexed | 2024-04-12T06:09:15Z |
| publishDate | 2019-06-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Microbiology |
| spelling | doaj.art-44ee9992716941f9a9f2092865afaea82022-12-22T03:44:45ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2019-06-011010.3389/fmicb.2019.01245449543Outbreaks of an Emerging Viral Disease Covary With Differences in the Composition of the Skin Microbiome of a Wild United Kingdom AmphibianLewis J. Campbell0Lewis J. Campbell1Trenton W. J. Garner2Kevin Hopkins3Amber G. F. Griffiths4Xavier A. Harrison5Xavier A. Harrison6Environment and Sustainability Institute, University of Exeter, Penryn, United KingdomInstitute of Zoology, Zoological Society of London, London, United KingdomInstitute of Zoology, Zoological Society of London, London, United KingdomInstitute of Zoology, Zoological Society of London, London, United KingdomFoAM Kernow, Penryn, United KingdomInstitute of Zoology, Zoological Society of London, London, United KingdomCollege of Life and Environmental Sciences, University of Exeter, Exeter, United KingdomThere is growing appreciation of the important role of commensal microbes in ensuring the normal function and health of their hosts, including determining how hosts respond to pathogens. A range of infectious diseases are threatening amphibians worldwide, and evidence is accumulating that the host-associated bacteria that comprise the microbiome may be key in mediating interactions between amphibian hosts and infectious pathogens. We used 16S rRNA amplicon sequencing to quantify the skin microbial community structure of over 200 individual wild adult European common frogs (Rana temporaria), from ten populations with contrasting history of the lethal disease ranavirosis, caused by emerging viral pathogens belonging to the genus Ranavirus. All populations had similar species richness irrespective of disease history, but populations that have experienced historical outbreaks of ranavirosis have a distinct skin microbiome structure (beta diversity) when compared to sites where no outbreaks of the disease have occurred. At the individual level, neither age, body length, nor sex of the frog could predict the structure of the skin microbiota. Our data potentially support the hypothesis that variation among individuals in skin microbiome structure drive differences in susceptibility to infection and lethal outbreaks of disease. More generally, our results suggest that population-level processes are more important for driving differences in microbiome structure than variation among individuals within populations in key life history traits such as age and body size.https://www.frontiersin.org/article/10.3389/fmicb.2019.01245/fulldiseaseamphibiansmicrobiomecommensal bacteriaranavirusRana temporaria |
| spellingShingle | Lewis J. Campbell Lewis J. Campbell Trenton W. J. Garner Kevin Hopkins Amber G. F. Griffiths Xavier A. Harrison Xavier A. Harrison Outbreaks of an Emerging Viral Disease Covary With Differences in the Composition of the Skin Microbiome of a Wild United Kingdom Amphibian Frontiers in Microbiology disease amphibians microbiome commensal bacteria ranavirus Rana temporaria |
| title | Outbreaks of an Emerging Viral Disease Covary With Differences in the Composition of the Skin Microbiome of a Wild United Kingdom Amphibian |
| title_full | Outbreaks of an Emerging Viral Disease Covary With Differences in the Composition of the Skin Microbiome of a Wild United Kingdom Amphibian |
| title_fullStr | Outbreaks of an Emerging Viral Disease Covary With Differences in the Composition of the Skin Microbiome of a Wild United Kingdom Amphibian |
| title_full_unstemmed | Outbreaks of an Emerging Viral Disease Covary With Differences in the Composition of the Skin Microbiome of a Wild United Kingdom Amphibian |
| title_short | Outbreaks of an Emerging Viral Disease Covary With Differences in the Composition of the Skin Microbiome of a Wild United Kingdom Amphibian |
| title_sort | outbreaks of an emerging viral disease covary with differences in the composition of the skin microbiome of a wild united kingdom amphibian |
| topic | disease amphibians microbiome commensal bacteria ranavirus Rana temporaria |
| url | https://www.frontiersin.org/article/10.3389/fmicb.2019.01245/full |
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