Effect of Insect Live Larvae as Environmental Enrichment on Poultry Gut Health: Gut Mucin Composition, Microbiota and Local Immune Response Evaluation
The aim of this study was to evaluate the effect of <i>Hermetia illucens</i> (HI) and <i>Tenebrio molitor</i> (TM) live larvae as environmental enrichment on the mucin composition, local immune response and microbiota of broilers. A total of 180 four-day-old male broiler chic...
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| Format: | Article |
| Language: | English |
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MDPI AG
2021-09-01
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| Series: | Animals |
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| Online Access: | https://www.mdpi.com/2076-2615/11/10/2819 |
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| author | Elena Colombino Ilaria Biasato Ilario Ferrocino Sara Bellezza Oddon Christian Caimi Marta Gariglio Sihem Dabbou Marta Caramori Elena Battisti Stefania Zanet Ezio Ferroglio Luca Cocolin Laura Gasco Achille Schiavone Maria Teresa Capucchio |
| author_facet | Elena Colombino Ilaria Biasato Ilario Ferrocino Sara Bellezza Oddon Christian Caimi Marta Gariglio Sihem Dabbou Marta Caramori Elena Battisti Stefania Zanet Ezio Ferroglio Luca Cocolin Laura Gasco Achille Schiavone Maria Teresa Capucchio |
| author_sort | Elena Colombino |
| collection | DOAJ |
| description | The aim of this study was to evaluate the effect of <i>Hermetia illucens</i> (HI) and <i>Tenebrio molitor</i> (TM) live larvae as environmental enrichment on the mucin composition, local immune response and microbiota of broilers. A total of 180 four-day-old male broiler chickens (Ross 308) were randomly allotted to three dietary treatments (six replicates/treatment; ten animals/replicate): (i) control (C); (ii) C+HI; (iii) C+TM. Live larvae were distributed based on 5% of the expected daily feed intake. At slaughter (39 days of age), samples of duodenum, jejunum and ileum (twelve animals/diet) were submitted to mucin histochemical evaluation. Expression of MUC-2 and cytokines was evaluated by rt-qPCR in jejunum. Mucin staining intensity was not influenced by diet (<i>p</i> > 0.05); however, this varied depending on the intestinal segment (<i>p</i> < 0.001). No significant differences were recorded for IL-4, IL-6 TNF-α, MUC-2 and INF-γ gene expression in jejunum, while IL-2 was lower in the TM group compared to HI and C (<i>p</i> = 0.044). Caecal microbiota showed higher abundance of <i>Clostridium, Saccharibacteria</i> and <i>Victivallaceae</i> in the HI group, while <i>Collinsella</i> was higher in the TM group. The results suggested that live insect larvae did not impair mucin composition or local immune response, and can slightly improve caecal microbiota by enhancing a minor fraction of short chain fatty acid-producing taxa. |
| first_indexed | 2024-03-10T06:47:08Z |
| format | Article |
| id | doaj.art-44eeb9dda1484f18817575a0a6941234 |
| institution | Directory Open Access Journal |
| issn | 2076-2615 |
| language | English |
| last_indexed | 2024-03-10T06:47:08Z |
| publishDate | 2021-09-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Animals |
| spelling | doaj.art-44eeb9dda1484f18817575a0a69412342023-11-22T17:09:36ZengMDPI AGAnimals2076-26152021-09-011110281910.3390/ani11102819Effect of Insect Live Larvae as Environmental Enrichment on Poultry Gut Health: Gut Mucin Composition, Microbiota and Local Immune Response EvaluationElena Colombino0Ilaria Biasato1Ilario Ferrocino2Sara Bellezza Oddon3Christian Caimi4Marta Gariglio5Sihem Dabbou6Marta Caramori7Elena Battisti8Stefania Zanet9Ezio Ferroglio10Luca Cocolin11Laura Gasco12Achille Schiavone13Maria Teresa Capucchio14Department of Veterinary Sciences, University of Turin, 10095 Grugliasco, TO, ItalyDepartment of Agricultural, Forestry and Food Sciences, University of Turin, 10095 Grugliasco, TO, ItalyDepartment of Agricultural, Forestry and Food Sciences, University of Turin, 10095 Grugliasco, TO, ItalyDepartment of Agricultural, Forestry and Food Sciences, University of Turin, 10095 Grugliasco, TO, ItalyDepartment of Agricultural, Forestry and Food Sciences, University of Turin, 10095 Grugliasco, TO, ItalyDepartment of Veterinary Sciences, University of Turin, 10095 Grugliasco, TO, ItalyCenter Agriculture Food Environment (C3A), University of Trento, 38010 San Michele all’Adige, TN, ItalyDepartment of Veterinary Sciences, University of Turin, 10095 Grugliasco, TO, ItalyDepartment of Veterinary Sciences, University of Turin, 10095 Grugliasco, TO, ItalyDepartment of Veterinary Sciences, University of Turin, 10095 Grugliasco, TO, ItalyDepartment of Veterinary Sciences, University of Turin, 10095 Grugliasco, TO, ItalyDepartment of Agricultural, Forestry and Food Sciences, University of Turin, 10095 Grugliasco, TO, ItalyDepartment of Agricultural, Forestry and Food Sciences, University of Turin, 10095 Grugliasco, TO, ItalyDepartment of Veterinary Sciences, University of Turin, 10095 Grugliasco, TO, ItalyDepartment of Veterinary Sciences, University of Turin, 10095 Grugliasco, TO, ItalyThe aim of this study was to evaluate the effect of <i>Hermetia illucens</i> (HI) and <i>Tenebrio molitor</i> (TM) live larvae as environmental enrichment on the mucin composition, local immune response and microbiota of broilers. A total of 180 four-day-old male broiler chickens (Ross 308) were randomly allotted to three dietary treatments (six replicates/treatment; ten animals/replicate): (i) control (C); (ii) C+HI; (iii) C+TM. Live larvae were distributed based on 5% of the expected daily feed intake. At slaughter (39 days of age), samples of duodenum, jejunum and ileum (twelve animals/diet) were submitted to mucin histochemical evaluation. Expression of MUC-2 and cytokines was evaluated by rt-qPCR in jejunum. Mucin staining intensity was not influenced by diet (<i>p</i> > 0.05); however, this varied depending on the intestinal segment (<i>p</i> < 0.001). No significant differences were recorded for IL-4, IL-6 TNF-α, MUC-2 and INF-γ gene expression in jejunum, while IL-2 was lower in the TM group compared to HI and C (<i>p</i> = 0.044). Caecal microbiota showed higher abundance of <i>Clostridium, Saccharibacteria</i> and <i>Victivallaceae</i> in the HI group, while <i>Collinsella</i> was higher in the TM group. The results suggested that live insect larvae did not impair mucin composition or local immune response, and can slightly improve caecal microbiota by enhancing a minor fraction of short chain fatty acid-producing taxa.https://www.mdpi.com/2076-2615/11/10/2819poultryinsect<i>Hermetia illucens</i><i>Tenebrio molitor</i>gut healthmucin |
| spellingShingle | Elena Colombino Ilaria Biasato Ilario Ferrocino Sara Bellezza Oddon Christian Caimi Marta Gariglio Sihem Dabbou Marta Caramori Elena Battisti Stefania Zanet Ezio Ferroglio Luca Cocolin Laura Gasco Achille Schiavone Maria Teresa Capucchio Effect of Insect Live Larvae as Environmental Enrichment on Poultry Gut Health: Gut Mucin Composition, Microbiota and Local Immune Response Evaluation Animals poultry insect <i>Hermetia illucens</i> <i>Tenebrio molitor</i> gut health mucin |
| title | Effect of Insect Live Larvae as Environmental Enrichment on Poultry Gut Health: Gut Mucin Composition, Microbiota and Local Immune Response Evaluation |
| title_full | Effect of Insect Live Larvae as Environmental Enrichment on Poultry Gut Health: Gut Mucin Composition, Microbiota and Local Immune Response Evaluation |
| title_fullStr | Effect of Insect Live Larvae as Environmental Enrichment on Poultry Gut Health: Gut Mucin Composition, Microbiota and Local Immune Response Evaluation |
| title_full_unstemmed | Effect of Insect Live Larvae as Environmental Enrichment on Poultry Gut Health: Gut Mucin Composition, Microbiota and Local Immune Response Evaluation |
| title_short | Effect of Insect Live Larvae as Environmental Enrichment on Poultry Gut Health: Gut Mucin Composition, Microbiota and Local Immune Response Evaluation |
| title_sort | effect of insect live larvae as environmental enrichment on poultry gut health gut mucin composition microbiota and local immune response evaluation |
| topic | poultry insect <i>Hermetia illucens</i> <i>Tenebrio molitor</i> gut health mucin |
| url | https://www.mdpi.com/2076-2615/11/10/2819 |
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