Effects of Fraxinellone on the Midgut Enzyme Activities of the 5th Instar Larvae of Oriental Armyworm, Mythimna separata Walker
Isolated from Dictamnus dasycarpus Turcz., fraxinellone exhibited multiple bioactivities against insects. In the present paper, the changes of digestive enzymes and detoxification enzymes of Mythimna separata Walker (5th instar larvae), treated with fraxinellone, were investigated. Compared with tho...
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| Format: | Article |
| Language: | English |
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MDPI AG
2014-09-01
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| Series: | Toxins |
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| Online Access: | http://www.mdpi.com/2072-6651/6/9/2708 |
| _version_ | 1828120754110070784 |
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| author | Min Lv Wenjun Wu Huixia Liu |
| author_facet | Min Lv Wenjun Wu Huixia Liu |
| author_sort | Min Lv |
| collection | DOAJ |
| description | Isolated from Dictamnus dasycarpus Turcz., fraxinellone exhibited multiple bioactivities against insects. In the present paper, the changes of digestive enzymes and detoxification enzymes of Mythimna separata Walker (5th instar larvae), treated with fraxinellone, were investigated. Compared with those of the control, the α-amylase activity of the fraxinellone-treated 5th instar larvae was inhibited, whereas the level of their protease activity was increased. Based upon further studies on the specific proteases, the levels of the active alkaline trypsin-like enzyme (BApNA as the substrate) and the chymotrypsin-like enzyme (BTEE as the substrate) activities of the treated larvae were declined; however, the level of activity of the weak alkaline trypsin-like enzyme (TAME as the substrate) of the treated ones was increased. Meanwhile, the activities of two detoxification enzymes, such as carboxylesterase (CarE) and glutathione S-transferase (GST), of the treated larvae were increased to some extent, but the activities of NADPH-P450 reductase and O-demethylase of the treated ones declined. Therefore, protease (especially the weak alkaline trypsin-like enzyme), CarE and GST played important roles in the metabolism of fraxinellone in the midgut of Mythimna separata (M. separata). |
| first_indexed | 2024-04-11T14:10:06Z |
| format | Article |
| id | doaj.art-44ef160990174d59900f8d95e6ea59f6 |
| institution | Directory Open Access Journal |
| issn | 2072-6651 |
| language | English |
| last_indexed | 2024-04-11T14:10:06Z |
| publishDate | 2014-09-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Toxins |
| spelling | doaj.art-44ef160990174d59900f8d95e6ea59f62022-12-22T04:19:43ZengMDPI AGToxins2072-66512014-09-01692708271810.3390/toxins6092708toxins6092708Effects of Fraxinellone on the Midgut Enzyme Activities of the 5th Instar Larvae of Oriental Armyworm, Mythimna separata WalkerMin Lv0Wenjun Wu1Huixia Liu2Institute of Pesticide Science, College of Plant Protection, Northwest A&F University, Yangling 712100, Shaanxi, ChinaInstitute of Pesticide Science, College of Plant Protection, Northwest A&F University, Yangling 712100, Shaanxi, ChinaInstitute of Pesticide Science, College of Plant Protection, Northwest A&F University, Yangling 712100, Shaanxi, ChinaIsolated from Dictamnus dasycarpus Turcz., fraxinellone exhibited multiple bioactivities against insects. In the present paper, the changes of digestive enzymes and detoxification enzymes of Mythimna separata Walker (5th instar larvae), treated with fraxinellone, were investigated. Compared with those of the control, the α-amylase activity of the fraxinellone-treated 5th instar larvae was inhibited, whereas the level of their protease activity was increased. Based upon further studies on the specific proteases, the levels of the active alkaline trypsin-like enzyme (BApNA as the substrate) and the chymotrypsin-like enzyme (BTEE as the substrate) activities of the treated larvae were declined; however, the level of activity of the weak alkaline trypsin-like enzyme (TAME as the substrate) of the treated ones was increased. Meanwhile, the activities of two detoxification enzymes, such as carboxylesterase (CarE) and glutathione S-transferase (GST), of the treated larvae were increased to some extent, but the activities of NADPH-P450 reductase and O-demethylase of the treated ones declined. Therefore, protease (especially the weak alkaline trypsin-like enzyme), CarE and GST played important roles in the metabolism of fraxinellone in the midgut of Mythimna separata (M. separata).http://www.mdpi.com/2072-6651/6/9/2708fraxinellonedigestive enzymedetoxification enzymeMythimna separata |
| spellingShingle | Min Lv Wenjun Wu Huixia Liu Effects of Fraxinellone on the Midgut Enzyme Activities of the 5th Instar Larvae of Oriental Armyworm, Mythimna separata Walker Toxins fraxinellone digestive enzyme detoxification enzyme Mythimna separata |
| title | Effects of Fraxinellone on the Midgut Enzyme Activities of the 5th Instar Larvae of Oriental Armyworm, Mythimna separata Walker |
| title_full | Effects of Fraxinellone on the Midgut Enzyme Activities of the 5th Instar Larvae of Oriental Armyworm, Mythimna separata Walker |
| title_fullStr | Effects of Fraxinellone on the Midgut Enzyme Activities of the 5th Instar Larvae of Oriental Armyworm, Mythimna separata Walker |
| title_full_unstemmed | Effects of Fraxinellone on the Midgut Enzyme Activities of the 5th Instar Larvae of Oriental Armyworm, Mythimna separata Walker |
| title_short | Effects of Fraxinellone on the Midgut Enzyme Activities of the 5th Instar Larvae of Oriental Armyworm, Mythimna separata Walker |
| title_sort | effects of fraxinellone on the midgut enzyme activities of the 5th instar larvae of oriental armyworm mythimna separata walker |
| topic | fraxinellone digestive enzyme detoxification enzyme Mythimna separata |
| url | http://www.mdpi.com/2072-6651/6/9/2708 |
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