Light and Ethephon Overcoming Seed Dormancy in Friar’s Crown (<i>Melocactus zehntneri</i>, Cactaceae), a Brazilian Cactus
Seed germination in <i>Melocactus</i> and other cactus species is hampered by dormancy. However, most studies failed to achieve high seed-germination rates, suggesting a complex mechanism of dormancy in Cactaceae. Thus, the objective of this study was to evaluate whether factors such as...
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MDPI AG
2023-12-01
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| Series: | Plants |
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| Online Access: | https://www.mdpi.com/2223-7747/12/24/4127 |
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| author | Mariana Freitas Campos Magnani Jean Carlos Cardoso |
| author_facet | Mariana Freitas Campos Magnani Jean Carlos Cardoso |
| author_sort | Mariana Freitas Campos Magnani |
| collection | DOAJ |
| description | Seed germination in <i>Melocactus</i> and other cactus species is hampered by dormancy. However, most studies failed to achieve high seed-germination rates, suggesting a complex mechanism of dormancy in Cactaceae. Thus, the objective of this study was to evaluate whether factors such as light and phytoregulators overcome the dormancy in the seeds of the friar’s crown cactus (<i>Melocactus zehntneri</i>). Two consecutive experimental sets were designed: one with seed germination under filter paper conditions and different wavelengths and Photosynthetically Photon Flux Densities (PPFDs); and one in vitro experiment using a culture medium to evaluate the influence of different phytoregulators, such as gibberellic acid (GA<sub>3</sub>), benzylaminopurine (BAP) and ethephon (ET), both in the germination of seeds of <i>M. zehntneri</i>. Seeds of <i>M. zehntneri</i> are positive photoblastic. Red light and gradual increases in PPFD resulted in the highest germination rates (60.8–61.7%) and germination speed index (4.4–4.5). In vitro seeding in culture media increased the germination percentage to 76% in control without phytoregulators. Ethephon showed a major effect in releasing the germination of dormant seeds of <i>M. zehntneri</i>, totaling 98% of seeds germinated under in vitro conditions, while GA<sub>3</sub> and BAP showed minor or no effect on germination. The present study resulted in an efficient in vitro technique for germination and a better understanding of cacti seed dormancy. |
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| language | English |
| last_indexed | 2024-03-08T18:34:39Z |
| publishDate | 2023-12-01 |
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| spelling | doaj.art-44f2e85e69c04ac789f93e98d6e5c9062023-12-29T15:47:27ZengMDPI AGPlants2223-77472023-12-011224412710.3390/plants12244127Light and Ethephon Overcoming Seed Dormancy in Friar’s Crown (<i>Melocactus zehntneri</i>, Cactaceae), a Brazilian CactusMariana Freitas Campos Magnani0Jean Carlos Cardoso1Research Group in Physiology Applied to Agriculture, Center of Agricultural Sciences, Federal University of São Carlos, Rodovia Anhanguera, km 174, Araras 13600-970, SP, BrazilLaboratory of Plant Physiology and Tissue Culture, Department of Biotechnology Plant and Animal Production, Center of Agricultural Sciences, Federal University of São Carlos, Rodovia Anhanguera, km 174, Araras 13600-970, SP, BrazilSeed germination in <i>Melocactus</i> and other cactus species is hampered by dormancy. However, most studies failed to achieve high seed-germination rates, suggesting a complex mechanism of dormancy in Cactaceae. Thus, the objective of this study was to evaluate whether factors such as light and phytoregulators overcome the dormancy in the seeds of the friar’s crown cactus (<i>Melocactus zehntneri</i>). Two consecutive experimental sets were designed: one with seed germination under filter paper conditions and different wavelengths and Photosynthetically Photon Flux Densities (PPFDs); and one in vitro experiment using a culture medium to evaluate the influence of different phytoregulators, such as gibberellic acid (GA<sub>3</sub>), benzylaminopurine (BAP) and ethephon (ET), both in the germination of seeds of <i>M. zehntneri</i>. Seeds of <i>M. zehntneri</i> are positive photoblastic. Red light and gradual increases in PPFD resulted in the highest germination rates (60.8–61.7%) and germination speed index (4.4–4.5). In vitro seeding in culture media increased the germination percentage to 76% in control without phytoregulators. Ethephon showed a major effect in releasing the germination of dormant seeds of <i>M. zehntneri</i>, totaling 98% of seeds germinated under in vitro conditions, while GA<sub>3</sub> and BAP showed minor or no effect on germination. The present study resulted in an efficient in vitro technique for germination and a better understanding of cacti seed dormancy.https://www.mdpi.com/2223-7747/12/24/4127cactusdormancy breakinggerminationlightphytoregulators |
| spellingShingle | Mariana Freitas Campos Magnani Jean Carlos Cardoso Light and Ethephon Overcoming Seed Dormancy in Friar’s Crown (<i>Melocactus zehntneri</i>, Cactaceae), a Brazilian Cactus Plants cactus dormancy breaking germination light phytoregulators |
| title | Light and Ethephon Overcoming Seed Dormancy in Friar’s Crown (<i>Melocactus zehntneri</i>, Cactaceae), a Brazilian Cactus |
| title_full | Light and Ethephon Overcoming Seed Dormancy in Friar’s Crown (<i>Melocactus zehntneri</i>, Cactaceae), a Brazilian Cactus |
| title_fullStr | Light and Ethephon Overcoming Seed Dormancy in Friar’s Crown (<i>Melocactus zehntneri</i>, Cactaceae), a Brazilian Cactus |
| title_full_unstemmed | Light and Ethephon Overcoming Seed Dormancy in Friar’s Crown (<i>Melocactus zehntneri</i>, Cactaceae), a Brazilian Cactus |
| title_short | Light and Ethephon Overcoming Seed Dormancy in Friar’s Crown (<i>Melocactus zehntneri</i>, Cactaceae), a Brazilian Cactus |
| title_sort | light and ethephon overcoming seed dormancy in friar s crown i melocactus zehntneri i cactaceae a brazilian cactus |
| topic | cactus dormancy breaking germination light phytoregulators |
| url | https://www.mdpi.com/2223-7747/12/24/4127 |
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