Novel autoantigens immunogenic in COPD patients

<p>Abstract</p> <p>Background</p> <p>Chronic obstructive pulmonary disease (COPD) is a respiratory inflammatory condition with autoimmune features including IgG autoantibodies. In this study we analyze the complexity of the autoantibody response and reveal the nature of...

Full description

Bibliographic Details
Main Authors: Stephan Bernhard, Huwer Hanno, Hamacher Jürg, Andres Claudia, Klein Veronika, Rheinheimer Stefanie, Ludwig Nicole, Heisel Sabrina, Keller Andreas, Leidinger Petra, Stehle Ingo, Lenhof Hans-Peter, Meese Eckart
Format: Article
Language:English
Published: BMC 2009-03-01
Series:Respiratory Research
Online Access:http://respiratory-research.com/content/10/1/20
_version_ 1818421140589117440
author Stephan Bernhard
Huwer Hanno
Hamacher Jürg
Andres Claudia
Klein Veronika
Rheinheimer Stefanie
Ludwig Nicole
Heisel Sabrina
Keller Andreas
Leidinger Petra
Stehle Ingo
Lenhof Hans-Peter
Meese Eckart
author_facet Stephan Bernhard
Huwer Hanno
Hamacher Jürg
Andres Claudia
Klein Veronika
Rheinheimer Stefanie
Ludwig Nicole
Heisel Sabrina
Keller Andreas
Leidinger Petra
Stehle Ingo
Lenhof Hans-Peter
Meese Eckart
author_sort Stephan Bernhard
collection DOAJ
description <p>Abstract</p> <p>Background</p> <p>Chronic obstructive pulmonary disease (COPD) is a respiratory inflammatory condition with autoimmune features including IgG autoantibodies. In this study we analyze the complexity of the autoantibody response and reveal the nature of the antigens that are recognized by autoantibodies in COPD patients.</p> <p>Methods</p> <p>An array of 1827 gridded immunogenic peptide clones was established and screened with 17 sera of COPD patients and 60 healthy controls. Protein arrays were evaluated both by visual inspection and a recently developed computer aided image analysis technique. By this computer aided image analysis technique we computed the intensity values for each peptide clone and each serum and calculated the area under the receiver operator characteristics curve (AUC) for each clone and the separation COPD sera versus control sera.</p> <p>Results</p> <p>By visual evaluation we detected 381 peptide clones that reacted with autoantibodies of COPD patients including 17 clones that reacted with more than 60% of the COPD sera and seven clones that reacted with more than 90% of the COPD sera. The comparison of COPD sera and controls by the automated image analysis system identified 212 peptide clones with informative AUC values. By <it>in silico </it>sequence analysis we found an enrichment of sequence motives previously associated with immunogenicity.</p> <p>Conclusion</p> <p>The identification of a rather complex humoral immune response in COPD patients supports the idea of COPD as a disease with strong autoimmune features. The identification of novel immunogenic antigens is a first step towards a better understanding of the autoimmune component of COPD.</p>
first_indexed 2024-12-14T13:05:38Z
format Article
id doaj.art-44f48384b86f4f5d821477861e0b7f05
institution Directory Open Access Journal
issn 1465-9921
language English
last_indexed 2024-12-14T13:05:38Z
publishDate 2009-03-01
publisher BMC
record_format Article
series Respiratory Research
spelling doaj.art-44f48384b86f4f5d821477861e0b7f052022-12-21T23:00:19ZengBMCRespiratory Research1465-99212009-03-011012010.1186/1465-9921-10-20Novel autoantigens immunogenic in COPD patientsStephan BernhardHuwer HannoHamacher JürgAndres ClaudiaKlein VeronikaRheinheimer StefanieLudwig NicoleHeisel SabrinaKeller AndreasLeidinger PetraStehle IngoLenhof Hans-PeterMeese Eckart<p>Abstract</p> <p>Background</p> <p>Chronic obstructive pulmonary disease (COPD) is a respiratory inflammatory condition with autoimmune features including IgG autoantibodies. In this study we analyze the complexity of the autoantibody response and reveal the nature of the antigens that are recognized by autoantibodies in COPD patients.</p> <p>Methods</p> <p>An array of 1827 gridded immunogenic peptide clones was established and screened with 17 sera of COPD patients and 60 healthy controls. Protein arrays were evaluated both by visual inspection and a recently developed computer aided image analysis technique. By this computer aided image analysis technique we computed the intensity values for each peptide clone and each serum and calculated the area under the receiver operator characteristics curve (AUC) for each clone and the separation COPD sera versus control sera.</p> <p>Results</p> <p>By visual evaluation we detected 381 peptide clones that reacted with autoantibodies of COPD patients including 17 clones that reacted with more than 60% of the COPD sera and seven clones that reacted with more than 90% of the COPD sera. The comparison of COPD sera and controls by the automated image analysis system identified 212 peptide clones with informative AUC values. By <it>in silico </it>sequence analysis we found an enrichment of sequence motives previously associated with immunogenicity.</p> <p>Conclusion</p> <p>The identification of a rather complex humoral immune response in COPD patients supports the idea of COPD as a disease with strong autoimmune features. The identification of novel immunogenic antigens is a first step towards a better understanding of the autoimmune component of COPD.</p>http://respiratory-research.com/content/10/1/20
spellingShingle Stephan Bernhard
Huwer Hanno
Hamacher Jürg
Andres Claudia
Klein Veronika
Rheinheimer Stefanie
Ludwig Nicole
Heisel Sabrina
Keller Andreas
Leidinger Petra
Stehle Ingo
Lenhof Hans-Peter
Meese Eckart
Novel autoantigens immunogenic in COPD patients
Respiratory Research
title Novel autoantigens immunogenic in COPD patients
title_full Novel autoantigens immunogenic in COPD patients
title_fullStr Novel autoantigens immunogenic in COPD patients
title_full_unstemmed Novel autoantigens immunogenic in COPD patients
title_short Novel autoantigens immunogenic in COPD patients
title_sort novel autoantigens immunogenic in copd patients
url http://respiratory-research.com/content/10/1/20
work_keys_str_mv AT stephanbernhard novelautoantigensimmunogenicincopdpatients
AT huwerhanno novelautoantigensimmunogenicincopdpatients
AT hamacherjurg novelautoantigensimmunogenicincopdpatients
AT andresclaudia novelautoantigensimmunogenicincopdpatients
AT kleinveronika novelautoantigensimmunogenicincopdpatients
AT rheinheimerstefanie novelautoantigensimmunogenicincopdpatients
AT ludwignicole novelautoantigensimmunogenicincopdpatients
AT heiselsabrina novelautoantigensimmunogenicincopdpatients
AT kellerandreas novelautoantigensimmunogenicincopdpatients
AT leidingerpetra novelautoantigensimmunogenicincopdpatients
AT stehleingo novelautoantigensimmunogenicincopdpatients
AT lenhofhanspeter novelautoantigensimmunogenicincopdpatients
AT meeseeckart novelautoantigensimmunogenicincopdpatients