Stability of lipids in plasma and serum: Effects of temperature-related storage conditions on the human lipidome
Large epidemiological studies often require sample transportation and storage, presenting unique considerations when applying advanced lipidomics techniques. The goal of this study was to acquire lipidomics data on plasma and serum samples stored at potential preanalytical conditions (e.g., thawing,...
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| Format: | Article |
| Language: | English |
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Elsevier
2021-11-01
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| Series: | Journal of Mass Spectrometry and Advances in the Clinical Lab |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2667145X21000225 |
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| author | Gregory B. Reis Jon C. Rees Anna A. Ivanova Zsuzsanna Kuklenyik Nathan M. Drew James L. Pirkle John R. Barr |
| author_facet | Gregory B. Reis Jon C. Rees Anna A. Ivanova Zsuzsanna Kuklenyik Nathan M. Drew James L. Pirkle John R. Barr |
| author_sort | Gregory B. Reis |
| collection | DOAJ |
| description | Large epidemiological studies often require sample transportation and storage, presenting unique considerations when applying advanced lipidomics techniques. The goal of this study was to acquire lipidomics data on plasma and serum samples stored at potential preanalytical conditions (e.g., thawing, extracting, evaporating), systematically monitoring lipid species for a period of one month. Split aliquots of 10 plasma samples and 10 serum samples from healthy individuals were kept in three temperature-related environments: refrigerator, laboratory benchtop, or heated incubator. Samples were analyzed at six different time points over 28 days using a Bligh & Dyer lipid extraction protocol followed by direct infusion into a lipidomics platform using differential mobility with tandem mass spectrometry. The observed concentration changes over time were evaluated relative to method and inter-individual biological variability. In addition, to evaluate the effect of lipase enzyme levels on concentration changes during storage, we compared corresponding fasting and post-prandial plasma samples collected from 5 individuals. Based on our data, a series of low abundance free fatty acid (FFA), diacylglycerol (DAG), and cholesteryl ester (CE) species were identified as potential analytical markers for degradation. These FFA and DAG species are typically produced by endogenous lipases from numerous triacylglycerols (TAGs), and certain high abundance phosphatidylcholines (PCs). The low concentration CEs, which appeared to increase several fold, were likely mass-isobars from oxidation of other high concentration CEs. Although the concentration changes of the high abundant TAG, PC, and CE precursors remained within method variability, the concentration trends of FFA, DAG, and oxidized CE products should be systematically monitored over time to inform analysts about possible pre-analytical biases due to degradation in the study sample sets. |
| first_indexed | 2024-12-14T23:21:13Z |
| format | Article |
| id | doaj.art-44f61992411e46f6b25f9c7ba08a2c73 |
| institution | Directory Open Access Journal |
| issn | 2667-145X |
| language | English |
| last_indexed | 2024-12-14T23:21:13Z |
| publishDate | 2021-11-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Mass Spectrometry and Advances in the Clinical Lab |
| spelling | doaj.art-44f61992411e46f6b25f9c7ba08a2c732022-12-21T22:43:57ZengElsevierJournal of Mass Spectrometry and Advances in the Clinical Lab2667-145X2021-11-01223442Stability of lipids in plasma and serum: Effects of temperature-related storage conditions on the human lipidomeGregory B. Reis0Jon C. Rees1Anna A. Ivanova2Zsuzsanna Kuklenyik3Nathan M. Drew4James L. Pirkle5John R. Barr6Division of Laboratory Sciences, National Center for Environmental Health, Centers for Disease Control and Prevention, Chamblee, GA, United StatesDivision of Laboratory Sciences, National Center for Environmental Health, Centers for Disease Control and Prevention, Chamblee, GA, United StatesDivision of Laboratory Sciences, National Center for Environmental Health, Centers for Disease Control and Prevention, Chamblee, GA, United StatesDivision of Laboratory Sciences, National Center for Environmental Health, Centers for Disease Control and Prevention, Chamblee, GA, United States; Corresponding author.Division of Science Integration, National Institute for Occupational Safety & Health, Centers for Disease Control and Prevention, Cincinnati, OH, United StatesDivision of Laboratory Sciences, National Center for Environmental Health, Centers for Disease Control and Prevention, Chamblee, GA, United StatesDivision of Laboratory Sciences, National Center for Environmental Health, Centers for Disease Control and Prevention, Chamblee, GA, United StatesLarge epidemiological studies often require sample transportation and storage, presenting unique considerations when applying advanced lipidomics techniques. The goal of this study was to acquire lipidomics data on plasma and serum samples stored at potential preanalytical conditions (e.g., thawing, extracting, evaporating), systematically monitoring lipid species for a period of one month. Split aliquots of 10 plasma samples and 10 serum samples from healthy individuals were kept in three temperature-related environments: refrigerator, laboratory benchtop, or heated incubator. Samples were analyzed at six different time points over 28 days using a Bligh & Dyer lipid extraction protocol followed by direct infusion into a lipidomics platform using differential mobility with tandem mass spectrometry. The observed concentration changes over time were evaluated relative to method and inter-individual biological variability. In addition, to evaluate the effect of lipase enzyme levels on concentration changes during storage, we compared corresponding fasting and post-prandial plasma samples collected from 5 individuals. Based on our data, a series of low abundance free fatty acid (FFA), diacylglycerol (DAG), and cholesteryl ester (CE) species were identified as potential analytical markers for degradation. These FFA and DAG species are typically produced by endogenous lipases from numerous triacylglycerols (TAGs), and certain high abundance phosphatidylcholines (PCs). The low concentration CEs, which appeared to increase several fold, were likely mass-isobars from oxidation of other high concentration CEs. Although the concentration changes of the high abundant TAG, PC, and CE precursors remained within method variability, the concentration trends of FFA, DAG, and oxidized CE products should be systematically monitored over time to inform analysts about possible pre-analytical biases due to degradation in the study sample sets.http://www.sciencedirect.com/science/article/pii/S2667145X21000225LipidomicsStabilityDegradationOxidationHydrolysisCholesteryl Ester |
| spellingShingle | Gregory B. Reis Jon C. Rees Anna A. Ivanova Zsuzsanna Kuklenyik Nathan M. Drew James L. Pirkle John R. Barr Stability of lipids in plasma and serum: Effects of temperature-related storage conditions on the human lipidome Journal of Mass Spectrometry and Advances in the Clinical Lab Lipidomics Stability Degradation Oxidation Hydrolysis Cholesteryl Ester |
| title | Stability of lipids in plasma and serum: Effects of temperature-related storage conditions on the human lipidome |
| title_full | Stability of lipids in plasma and serum: Effects of temperature-related storage conditions on the human lipidome |
| title_fullStr | Stability of lipids in plasma and serum: Effects of temperature-related storage conditions on the human lipidome |
| title_full_unstemmed | Stability of lipids in plasma and serum: Effects of temperature-related storage conditions on the human lipidome |
| title_short | Stability of lipids in plasma and serum: Effects of temperature-related storage conditions on the human lipidome |
| title_sort | stability of lipids in plasma and serum effects of temperature related storage conditions on the human lipidome |
| topic | Lipidomics Stability Degradation Oxidation Hydrolysis Cholesteryl Ester |
| url | http://www.sciencedirect.com/science/article/pii/S2667145X21000225 |
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