Quality of sperm from cryopreserved semen of Tor soro in Dimethylsulfoxide and Glycerol 5, 10 and 15%
<p>Attempt to produce a lot number of <em>Tor soro</em> is difficult if only obtained by natural spawning since they often get mature in dissimilar time. Stock of sperm kept in enough number for fertilization in anytime is needed to overcome the problem. Cryopreservation of semen...
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| Format: | Article |
| Language: | English |
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Asosiasi Sains Akuakultur Indonesia
2007-07-01
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| Series: | Jurnal Akuakultur Indonesia |
| Online Access: | http://journal.ipb.ac.id/index.php/jai/article/view/3944 |
| _version_ | 1811339121368498176 |
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| author | M. Zairin Junior S. Handayani I. Supriatna |
| author_facet | M. Zairin Junior S. Handayani I. Supriatna |
| author_sort | M. Zairin Junior |
| collection | DOAJ |
| description | <p>Attempt to produce a lot number of <em>Tor soro</em> is difficult if only obtained by natural spawning since they often get mature in dissimilar time. Stock of sperm kept in enough number for fertilization in anytime is needed to overcome the problem. Cryopreservation of semen is one of sperm cryopreservation methods using liquid nitrogen (-196<sup>o</sup>C) to quickly freeze the sperm. Several cryoprotectants can be utilized in cryopreservation. In this study, quality by means of motility of cryopreserved sperm using dimethylsulfoxide or glycerol as cryoprotectant in the dose of 5, 10 and 15% were analyzed. The result of study show that percentage of sperm motile after cryopreservation using dimethylsulfoxide and glycerol was similar ranged from 76.7 to 83.3%. Therefore, both cryoprotectant used in this study could be employed for cryopreservation of Tor soro sperm.</p> <p>Keywords: cryopreservation, dimethylsulfoxide, glycerol, sperm, <em>Tor soro</em></p> <p><strong> </strong></p> <p><strong>ABSTRAK</strong></p> <p>Upaya produksi ikan batak (<em>Tor soro</em>) dalam jumlah besar relatif sulit jika hanya mengandalkan hasil pemijahan secara alami karena kematangan gonad antara ikan jantan dan betina sering tidak bersamaan. Untuk mengatasinya diperlukan stok sperma yang disimpan dalam jumlah yang cukup banyak untuk pembuahan yang dapat dipakai setiap saat. Kriopreservasi semen merupakan salah satu cara penyimpanan sperma yang menggunakan larutan nitrogen cair (-196°C) untuk membekukan sperma secara cepat. Beberapa jenis protektan dapat digunakan dalam pembekuan sperma. Pada penelitian ini, dilakukan analisis kualitas dalam arti motilitas sperma hasil kriopreservasi menggunakan dimetilsulfoksida dan gliserol sebagai krioprotektan dengan dosis 5, 10 dan 15%. Hasil penelitian menunjukkan bahwa persentase sperma motil setelah dilakukan kriopreservasi adalah sama, berkisar antara 76,7% hingga 83,35%. Dengan demikian, kedua jenis krioprotektan yang digunakan dalam penelitian ini bisa dipakai dalam kriopreservasi sperma ikan batak.</p> <p>Kata kunci: kriopreservasi, dimetilsulfoksida, gliserol, sperma, <em>Tor soro</em></p> |
| first_indexed | 2024-04-13T18:21:30Z |
| format | Article |
| id | doaj.art-44f6e783ca0e45fd8124d3d08633628b |
| institution | Directory Open Access Journal |
| issn | 1412-5269 2354-6700 |
| language | English |
| last_indexed | 2024-04-13T18:21:30Z |
| publishDate | 2007-07-01 |
| publisher | Asosiasi Sains Akuakultur Indonesia |
| record_format | Article |
| series | Jurnal Akuakultur Indonesia |
| spelling | doaj.art-44f6e783ca0e45fd8124d3d08633628b2022-12-22T02:35:26ZengAsosiasi Sains Akuakultur IndonesiaJurnal Akuakultur Indonesia1412-52692354-67002007-07-014214515110.19027/jai.4.145-1513556Quality of sperm from cryopreserved semen of Tor soro in Dimethylsulfoxide and Glycerol 5, 10 and 15%M. Zairin Junior0S. Handayani1I. Supriatna2Bogor Agricultural University, Department of AquacultureBogor Agricultural University, Department of AquacultureDepartemen Reproduksi dan Kebidanan, Fakultas Kedokteran Hewan, Institut Pertanian Bogor<p>Attempt to produce a lot number of <em>Tor soro</em> is difficult if only obtained by natural spawning since they often get mature in dissimilar time. Stock of sperm kept in enough number for fertilization in anytime is needed to overcome the problem. Cryopreservation of semen is one of sperm cryopreservation methods using liquid nitrogen (-196<sup>o</sup>C) to quickly freeze the sperm. Several cryoprotectants can be utilized in cryopreservation. In this study, quality by means of motility of cryopreserved sperm using dimethylsulfoxide or glycerol as cryoprotectant in the dose of 5, 10 and 15% were analyzed. The result of study show that percentage of sperm motile after cryopreservation using dimethylsulfoxide and glycerol was similar ranged from 76.7 to 83.3%. Therefore, both cryoprotectant used in this study could be employed for cryopreservation of Tor soro sperm.</p> <p>Keywords: cryopreservation, dimethylsulfoxide, glycerol, sperm, <em>Tor soro</em></p> <p><strong> </strong></p> <p><strong>ABSTRAK</strong></p> <p>Upaya produksi ikan batak (<em>Tor soro</em>) dalam jumlah besar relatif sulit jika hanya mengandalkan hasil pemijahan secara alami karena kematangan gonad antara ikan jantan dan betina sering tidak bersamaan. Untuk mengatasinya diperlukan stok sperma yang disimpan dalam jumlah yang cukup banyak untuk pembuahan yang dapat dipakai setiap saat. Kriopreservasi semen merupakan salah satu cara penyimpanan sperma yang menggunakan larutan nitrogen cair (-196°C) untuk membekukan sperma secara cepat. Beberapa jenis protektan dapat digunakan dalam pembekuan sperma. Pada penelitian ini, dilakukan analisis kualitas dalam arti motilitas sperma hasil kriopreservasi menggunakan dimetilsulfoksida dan gliserol sebagai krioprotektan dengan dosis 5, 10 dan 15%. Hasil penelitian menunjukkan bahwa persentase sperma motil setelah dilakukan kriopreservasi adalah sama, berkisar antara 76,7% hingga 83,35%. Dengan demikian, kedua jenis krioprotektan yang digunakan dalam penelitian ini bisa dipakai dalam kriopreservasi sperma ikan batak.</p> <p>Kata kunci: kriopreservasi, dimetilsulfoksida, gliserol, sperma, <em>Tor soro</em></p>http://journal.ipb.ac.id/index.php/jai/article/view/3944 |
| spellingShingle | M. Zairin Junior S. Handayani I. Supriatna Quality of sperm from cryopreserved semen of Tor soro in Dimethylsulfoxide and Glycerol 5, 10 and 15% Jurnal Akuakultur Indonesia |
| title | Quality of sperm from cryopreserved semen of Tor soro in Dimethylsulfoxide and Glycerol 5, 10 and 15% |
| title_full | Quality of sperm from cryopreserved semen of Tor soro in Dimethylsulfoxide and Glycerol 5, 10 and 15% |
| title_fullStr | Quality of sperm from cryopreserved semen of Tor soro in Dimethylsulfoxide and Glycerol 5, 10 and 15% |
| title_full_unstemmed | Quality of sperm from cryopreserved semen of Tor soro in Dimethylsulfoxide and Glycerol 5, 10 and 15% |
| title_short | Quality of sperm from cryopreserved semen of Tor soro in Dimethylsulfoxide and Glycerol 5, 10 and 15% |
| title_sort | quality of sperm from cryopreserved semen of tor soro in dimethylsulfoxide and glycerol 5 10 and 15 |
| url | http://journal.ipb.ac.id/index.php/jai/article/view/3944 |
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