Assessing the bipotency of in vitro-derived neuromesodermal progenitors [v1; ref status: indexed, http://f1000r.es/58z]
Retrospective clonal analysis in the mouse has demonstrated that the posterior spinal cord neurectoderm and paraxial mesoderm share a common bipotent progenitor. These neuromesodermal progenitors (NMPs) are the source of new axial structures during embryonic rostrocaudal axis elongation and are mark...
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| Format: | Article |
| Language: | English |
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F1000 Research Ltd
2015-04-01
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| Series: | F1000Research |
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| Online Access: | http://f1000research.com/articles/4-100/v1 |
| _version_ | 1819025443480666112 |
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| author | Anestis Tsakiridis Valerie Wilson |
| author_facet | Anestis Tsakiridis Valerie Wilson |
| author_sort | Anestis Tsakiridis |
| collection | DOAJ |
| description | Retrospective clonal analysis in the mouse has demonstrated that the posterior spinal cord neurectoderm and paraxial mesoderm share a common bipotent progenitor. These neuromesodermal progenitors (NMPs) are the source of new axial structures during embryonic rostrocaudal axis elongation and are marked by the simultaneous co-expression of the transcription factors T(Brachyury) (T(Bra)) and Sox2. NMP-like cells have recently been derived from pluripotent stem cells in vitro following combined stimulation of Wnt and fibroblast growth factor (FGF) signaling. Under these conditions the majority of cultures consist of T(Bra)/Sox2 co-expressing cells after 48-72 hours of differentiation. Although the capacity of these cells to generate posterior neural and paraxial mesoderm derivatives has been demonstrated at the population level, it is unknown whether a single in vitro-derived NMP can give rise to both neural and mesodermal cells. Here we demonstrate that T(Bra) positive cells obtained from mouse epiblast stem cells (EpiSCs) after culture in NMP-inducing conditions can generate both neural and mesodermal clones. This finding suggests that, similar to their embryonic counterparts, in vitro-derived NMPs are truly bipotent and can thus be exploited as a model for studying the molecular basis of developmental cell fate decisions. |
| first_indexed | 2024-12-21T05:10:46Z |
| format | Article |
| id | doaj.art-44f8b45108f147259f3bb5e1f6aa2f2a |
| institution | Directory Open Access Journal |
| issn | 2046-1402 |
| language | English |
| last_indexed | 2024-12-21T05:10:46Z |
| publishDate | 2015-04-01 |
| publisher | F1000 Research Ltd |
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| spelling | doaj.art-44f8b45108f147259f3bb5e1f6aa2f2a2022-12-21T19:15:03ZengF1000 Research LtdF1000Research2046-14022015-04-01410.12688/f1000research.6345.16803Assessing the bipotency of in vitro-derived neuromesodermal progenitors [v1; ref status: indexed, http://f1000r.es/58z]Anestis Tsakiridis0Valerie Wilson1MRC Centre for Regenerative Medicine, Institute for Stem Cell Research, School of Biological Sciences, University of Edinburgh, Edinburgh, EH16 4UU, UKMRC Centre for Regenerative Medicine, Institute for Stem Cell Research, School of Biological Sciences, University of Edinburgh, Edinburgh, EH16 4UU, UKRetrospective clonal analysis in the mouse has demonstrated that the posterior spinal cord neurectoderm and paraxial mesoderm share a common bipotent progenitor. These neuromesodermal progenitors (NMPs) are the source of new axial structures during embryonic rostrocaudal axis elongation and are marked by the simultaneous co-expression of the transcription factors T(Brachyury) (T(Bra)) and Sox2. NMP-like cells have recently been derived from pluripotent stem cells in vitro following combined stimulation of Wnt and fibroblast growth factor (FGF) signaling. Under these conditions the majority of cultures consist of T(Bra)/Sox2 co-expressing cells after 48-72 hours of differentiation. Although the capacity of these cells to generate posterior neural and paraxial mesoderm derivatives has been demonstrated at the population level, it is unknown whether a single in vitro-derived NMP can give rise to both neural and mesodermal cells. Here we demonstrate that T(Bra) positive cells obtained from mouse epiblast stem cells (EpiSCs) after culture in NMP-inducing conditions can generate both neural and mesodermal clones. This finding suggests that, similar to their embryonic counterparts, in vitro-derived NMPs are truly bipotent and can thus be exploited as a model for studying the molecular basis of developmental cell fate decisions.http://f1000research.com/articles/4-100/v1Cell SignalingControl of Gene ExpressionMorphogenesis & Cell BiologyStem Cells & Regeneration |
| spellingShingle | Anestis Tsakiridis Valerie Wilson Assessing the bipotency of in vitro-derived neuromesodermal progenitors [v1; ref status: indexed, http://f1000r.es/58z] F1000Research Cell Signaling Control of Gene Expression Morphogenesis & Cell Biology Stem Cells & Regeneration |
| title | Assessing the bipotency of in vitro-derived neuromesodermal progenitors [v1; ref status: indexed, http://f1000r.es/58z] |
| title_full | Assessing the bipotency of in vitro-derived neuromesodermal progenitors [v1; ref status: indexed, http://f1000r.es/58z] |
| title_fullStr | Assessing the bipotency of in vitro-derived neuromesodermal progenitors [v1; ref status: indexed, http://f1000r.es/58z] |
| title_full_unstemmed | Assessing the bipotency of in vitro-derived neuromesodermal progenitors [v1; ref status: indexed, http://f1000r.es/58z] |
| title_short | Assessing the bipotency of in vitro-derived neuromesodermal progenitors [v1; ref status: indexed, http://f1000r.es/58z] |
| title_sort | assessing the bipotency of in vitro derived neuromesodermal progenitors v1 ref status indexed http f1000r es 58z |
| topic | Cell Signaling Control of Gene Expression Morphogenesis & Cell Biology Stem Cells & Regeneration |
| url | http://f1000research.com/articles/4-100/v1 |
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