Human Adipose Stem Cells (hASCs) Grown on Biodegradable Microcarriers in Serum- and Xeno-Free Medium Preserve Their Undifferentiated Status
Human adipose stem cells (hASCs) are promising candidates for cell-based therapies, but they need to be efficiently expanded in vitro as they cannot be harvested in sufficient quantities. Recently, dynamic bioreactor systems operated with microcarriers achieved considerable high cell densities. Thus...
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| Format: | Article |
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MDPI AG
2021-04-01
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| Series: | Journal of Functional Biomaterials |
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| Online Access: | https://www.mdpi.com/2079-4983/12/2/25 |
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| author | Francesco Muoio Stefano Panella Valentin Jossen Matias Lindner Yves Harder Michele Müller Regine Eibl Tiziano Tallone |
| author_facet | Francesco Muoio Stefano Panella Valentin Jossen Matias Lindner Yves Harder Michele Müller Regine Eibl Tiziano Tallone |
| author_sort | Francesco Muoio |
| collection | DOAJ |
| description | Human adipose stem cells (hASCs) are promising candidates for cell-based therapies, but they need to be efficiently expanded in vitro as they cannot be harvested in sufficient quantities. Recently, dynamic bioreactor systems operated with microcarriers achieved considerable high cell densities. Thus, they are a viable alternative to static planar cultivation systems to obtain high numbers of clinical-grade hASCs. Nevertheless, the production of considerable biomass in a short time must not be achieved to the detriment of the cells’ quality. To facilitate the scalable expansion of hASC, we have developed a new serum- and xeno-free medium (<i>UrSuppe</i>) and a biodegradable microcarrier (<i>BR44</i>). In this study, we investigated whether the culture of hASCs in defined serum-free conditions on microcarriers (3D) or on planar (2D) cell culture vessels may influence the expression of some marker genes linked with the immature degree or the differentiated status of the cells. Furthermore, we investigated whether the biomaterials, which form our biodegradable MCs, may affect cell behavior and differentiation. The results confirmed that the quality and the undifferentiated status of the hASCs are very well preserved when they grow on <i>BR44</i> MCs in defined serum-free conditions. Indeed, the ASCs showed a gene expression profile more compatible with an undifferentiated status than the same cells grown under standard planar conditions. |
| first_indexed | 2024-03-10T12:16:15Z |
| format | Article |
| id | doaj.art-44faa8b428ef48218f2ec66ce6eb7c58 |
| institution | Directory Open Access Journal |
| issn | 2079-4983 |
| language | English |
| last_indexed | 2024-03-10T12:16:15Z |
| publishDate | 2021-04-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Journal of Functional Biomaterials |
| spelling | doaj.art-44faa8b428ef48218f2ec66ce6eb7c582023-11-21T15:49:35ZengMDPI AGJournal of Functional Biomaterials2079-49832021-04-011222510.3390/jfb12020025Human Adipose Stem Cells (hASCs) Grown on Biodegradable Microcarriers in Serum- and Xeno-Free Medium Preserve Their Undifferentiated StatusFrancesco Muoio0Stefano Panella1Valentin Jossen2Matias Lindner3Yves Harder4Michele Müller5Regine Eibl6Tiziano Tallone7Foundation for Cardiological Research and Education (FCRE), Cardiocentro Ticino Foundation, 6807 Taverne, SwitzerlandFoundation for Cardiological Research and Education (FCRE), Cardiocentro Ticino Foundation, 6807 Taverne, SwitzerlandInstitute of Chemistry & Biotechnology, Competence Center of Biochemical Engineering & Cell Cultivation Technique Zurich University of Applied Sciences, 8820 Wädenswil, SwitzerlandSferalp SA, 6998 Monteggio, SwitzerlandDepartment of Plastic, Reconstructive and Aesthetic Surgery, EOC, 6900 Lugano, SwitzerlandSferalp SA, 6998 Monteggio, SwitzerlandInstitute of Chemistry & Biotechnology, Competence Center of Biochemical Engineering & Cell Cultivation Technique Zurich University of Applied Sciences, 8820 Wädenswil, SwitzerlandFoundation for Cardiological Research and Education (FCRE), Cardiocentro Ticino Foundation, 6807 Taverne, SwitzerlandHuman adipose stem cells (hASCs) are promising candidates for cell-based therapies, but they need to be efficiently expanded in vitro as they cannot be harvested in sufficient quantities. Recently, dynamic bioreactor systems operated with microcarriers achieved considerable high cell densities. Thus, they are a viable alternative to static planar cultivation systems to obtain high numbers of clinical-grade hASCs. Nevertheless, the production of considerable biomass in a short time must not be achieved to the detriment of the cells’ quality. To facilitate the scalable expansion of hASC, we have developed a new serum- and xeno-free medium (<i>UrSuppe</i>) and a biodegradable microcarrier (<i>BR44</i>). In this study, we investigated whether the culture of hASCs in defined serum-free conditions on microcarriers (3D) or on planar (2D) cell culture vessels may influence the expression of some marker genes linked with the immature degree or the differentiated status of the cells. Furthermore, we investigated whether the biomaterials, which form our biodegradable MCs, may affect cell behavior and differentiation. The results confirmed that the quality and the undifferentiated status of the hASCs are very well preserved when they grow on <i>BR44</i> MCs in defined serum-free conditions. Indeed, the ASCs showed a gene expression profile more compatible with an undifferentiated status than the same cells grown under standard planar conditions.https://www.mdpi.com/2079-4983/12/2/25serum- and xeno-free cell culture medium<i>UrSuppe</i>biodegradable microcarrier (MC)biomaterialhuman adipose stem cells (hASCs) |
| spellingShingle | Francesco Muoio Stefano Panella Valentin Jossen Matias Lindner Yves Harder Michele Müller Regine Eibl Tiziano Tallone Human Adipose Stem Cells (hASCs) Grown on Biodegradable Microcarriers in Serum- and Xeno-Free Medium Preserve Their Undifferentiated Status Journal of Functional Biomaterials serum- and xeno-free cell culture medium <i>UrSuppe</i> biodegradable microcarrier (MC) biomaterial human adipose stem cells (hASCs) |
| title | Human Adipose Stem Cells (hASCs) Grown on Biodegradable Microcarriers in Serum- and Xeno-Free Medium Preserve Their Undifferentiated Status |
| title_full | Human Adipose Stem Cells (hASCs) Grown on Biodegradable Microcarriers in Serum- and Xeno-Free Medium Preserve Their Undifferentiated Status |
| title_fullStr | Human Adipose Stem Cells (hASCs) Grown on Biodegradable Microcarriers in Serum- and Xeno-Free Medium Preserve Their Undifferentiated Status |
| title_full_unstemmed | Human Adipose Stem Cells (hASCs) Grown on Biodegradable Microcarriers in Serum- and Xeno-Free Medium Preserve Their Undifferentiated Status |
| title_short | Human Adipose Stem Cells (hASCs) Grown on Biodegradable Microcarriers in Serum- and Xeno-Free Medium Preserve Their Undifferentiated Status |
| title_sort | human adipose stem cells hascs grown on biodegradable microcarriers in serum and xeno free medium preserve their undifferentiated status |
| topic | serum- and xeno-free cell culture medium <i>UrSuppe</i> biodegradable microcarrier (MC) biomaterial human adipose stem cells (hASCs) |
| url | https://www.mdpi.com/2079-4983/12/2/25 |
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