Early detection of neutralizing antibodies against SARS-CoV-2 in COVID-19 patients in Thailand.
<h4>Background</h4>The presence of neutralizing antibodies (NAbs) is an indicator of protective immunity for most viral infections. A newly developed surrogate viral neutralization assay (sVNT) offers the ability to detect total receptor binding domain-targeting NAbs in an isotype-indepe...
| Main Authors: | , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Public Library of Science (PLoS)
2021-01-01
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| Series: | PLoS ONE |
| Online Access: | https://doi.org/10.1371/journal.pone.0246864 |
| _version_ | 1819025562571636736 |
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| author | Opass Putcharoen Supaporn Wacharapluesadee Wan Ni Chia Leilani Paitoonpong Chee Wah Tan Gompol Suwanpimolkul Watsamon Jantarabenjakul Chanida Ruchisrisarod Phanni Wanthong Jiratchaya Sophonphan Pajaree Chariyavilaskul Lin-Fa Wang Thiravat Hemachudha |
| author_facet | Opass Putcharoen Supaporn Wacharapluesadee Wan Ni Chia Leilani Paitoonpong Chee Wah Tan Gompol Suwanpimolkul Watsamon Jantarabenjakul Chanida Ruchisrisarod Phanni Wanthong Jiratchaya Sophonphan Pajaree Chariyavilaskul Lin-Fa Wang Thiravat Hemachudha |
| author_sort | Opass Putcharoen |
| collection | DOAJ |
| description | <h4>Background</h4>The presence of neutralizing antibodies (NAbs) is an indicator of protective immunity for most viral infections. A newly developed surrogate viral neutralization assay (sVNT) offers the ability to detect total receptor binding domain-targeting NAbs in an isotype-independent manner, increasing the test sensitivity. Thus, specimens with low IgM/ IgG antibody levels showed strong neutralization activity in sVNT.<h4>Methods</h4>This study aimed to measure the %inhibition of NAbs measured by sVNT in PCR-confirmed COVID-19 patients. The sensitivity of sVNT for the diagnosis of SARS-CoV-2 infection and its kinetics were determined.<h4>Results</h4>Ninety-seven patients with PCR-confirmed SARS-CoV-2 infection were included in this study. Majority of the patients were 21-40 years old (67%) and 63% had mild symptoms. The sensitivity of sVNT for the diagnosis of SARS-CoV-2 infection was 99% (95% confidence interval (CI) 94.4-100%) and the specificity was 100% (95% CI 98.3-100%). The negative predictive value of sVNT from the samples collected before and after 7 days of symptom onset was 99.5% (95% CI 97.4-100%) and 100% (95% CI 93.8-100%), respectively. The level of inhibition at days 8-14 were significantly higher than days 0-7 (p<0.001). The median %inhibition values by severity of COVID-19 symptoms were 79.9% (interquartile range (IQR) 49.7-91.8%); 89.0% (IQR 71.2-92.4%); and 86.6% (IQR 69.5-92.8%), for mild, moderate and severe/critical symptoms respectively. The median level of sVNT %inhibition of severe was significantly higher than the mild group (p = 0.05).<h4>Conclusion</h4>The sVNT is a practical and robust serological test for SARS-CoV-2 infection and does not require specialized biosafety containment. It can be used clinically to aid diagnosis in both early and late infection especially in cases when the real-time RT-PCR results in weakly negative or weakly positive, and to determine the protective immune response from SARS-CoV-2 infection in patients. |
| first_indexed | 2024-12-21T05:12:39Z |
| format | Article |
| id | doaj.art-44fb7a80fb0643fba8f68231d73524c4 |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-12-21T05:12:39Z |
| publishDate | 2021-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-44fb7a80fb0643fba8f68231d73524c42022-12-21T19:15:00ZengPublic Library of Science (PLoS)PLoS ONE1932-62032021-01-01162e024686410.1371/journal.pone.0246864Early detection of neutralizing antibodies against SARS-CoV-2 in COVID-19 patients in Thailand.Opass PutcharoenSupaporn WacharapluesadeeWan Ni ChiaLeilani PaitoonpongChee Wah TanGompol SuwanpimolkulWatsamon JantarabenjakulChanida RuchisrisarodPhanni WanthongJiratchaya SophonphanPajaree ChariyavilaskulLin-Fa WangThiravat Hemachudha<h4>Background</h4>The presence of neutralizing antibodies (NAbs) is an indicator of protective immunity for most viral infections. A newly developed surrogate viral neutralization assay (sVNT) offers the ability to detect total receptor binding domain-targeting NAbs in an isotype-independent manner, increasing the test sensitivity. Thus, specimens with low IgM/ IgG antibody levels showed strong neutralization activity in sVNT.<h4>Methods</h4>This study aimed to measure the %inhibition of NAbs measured by sVNT in PCR-confirmed COVID-19 patients. The sensitivity of sVNT for the diagnosis of SARS-CoV-2 infection and its kinetics were determined.<h4>Results</h4>Ninety-seven patients with PCR-confirmed SARS-CoV-2 infection were included in this study. Majority of the patients were 21-40 years old (67%) and 63% had mild symptoms. The sensitivity of sVNT for the diagnosis of SARS-CoV-2 infection was 99% (95% confidence interval (CI) 94.4-100%) and the specificity was 100% (95% CI 98.3-100%). The negative predictive value of sVNT from the samples collected before and after 7 days of symptom onset was 99.5% (95% CI 97.4-100%) and 100% (95% CI 93.8-100%), respectively. The level of inhibition at days 8-14 were significantly higher than days 0-7 (p<0.001). The median %inhibition values by severity of COVID-19 symptoms were 79.9% (interquartile range (IQR) 49.7-91.8%); 89.0% (IQR 71.2-92.4%); and 86.6% (IQR 69.5-92.8%), for mild, moderate and severe/critical symptoms respectively. The median level of sVNT %inhibition of severe was significantly higher than the mild group (p = 0.05).<h4>Conclusion</h4>The sVNT is a practical and robust serological test for SARS-CoV-2 infection and does not require specialized biosafety containment. It can be used clinically to aid diagnosis in both early and late infection especially in cases when the real-time RT-PCR results in weakly negative or weakly positive, and to determine the protective immune response from SARS-CoV-2 infection in patients.https://doi.org/10.1371/journal.pone.0246864 |
| spellingShingle | Opass Putcharoen Supaporn Wacharapluesadee Wan Ni Chia Leilani Paitoonpong Chee Wah Tan Gompol Suwanpimolkul Watsamon Jantarabenjakul Chanida Ruchisrisarod Phanni Wanthong Jiratchaya Sophonphan Pajaree Chariyavilaskul Lin-Fa Wang Thiravat Hemachudha Early detection of neutralizing antibodies against SARS-CoV-2 in COVID-19 patients in Thailand. PLoS ONE |
| title | Early detection of neutralizing antibodies against SARS-CoV-2 in COVID-19 patients in Thailand. |
| title_full | Early detection of neutralizing antibodies against SARS-CoV-2 in COVID-19 patients in Thailand. |
| title_fullStr | Early detection of neutralizing antibodies against SARS-CoV-2 in COVID-19 patients in Thailand. |
| title_full_unstemmed | Early detection of neutralizing antibodies against SARS-CoV-2 in COVID-19 patients in Thailand. |
| title_short | Early detection of neutralizing antibodies against SARS-CoV-2 in COVID-19 patients in Thailand. |
| title_sort | early detection of neutralizing antibodies against sars cov 2 in covid 19 patients in thailand |
| url | https://doi.org/10.1371/journal.pone.0246864 |
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