Multiplication, histodifferentiation and regeneration of embryogenic cell suspensión on the plantain cultivar ‘Navolean’ (AAB)

The results obtained show that the best cell density for the multiplication of the cell suspensions in the cultivar ‘Navolean’ is 3.0% settled cell volume. In the histo-differentiation phase the greatest formation of somatic embryos in the globular stage was obtained using a density of 12.0% final c...

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Main Authors: Manuel Cabrera, Jorge López, Rafael Gómez-Kosky, Nery Montano, Maritza Reyes, D. Reinaldo, José de la C. Ventura, Victor Medero, Arletys Santos, M. García, Milagros Basail, E. Espinosa
Format: Article
Language:English
Published: Universidad Central Marta Abreu de Las Villas 2002-04-01
Series:Biotecnología Vegetal
Online Access:https://revista.ibp.co.cu/index.php/BV/article/view/155
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author Manuel Cabrera
Jorge López
Rafael Gómez-Kosky
Nery Montano
Maritza Reyes
D. Reinaldo
José de la C. Ventura
Victor Medero
Arletys Santos
M. García
Milagros Basail
E. Espinosa
author_facet Manuel Cabrera
Jorge López
Rafael Gómez-Kosky
Nery Montano
Maritza Reyes
D. Reinaldo
José de la C. Ventura
Victor Medero
Arletys Santos
M. García
Milagros Basail
E. Espinosa
author_sort Manuel Cabrera
collection DOAJ
description The results obtained show that the best cell density for the multiplication of the cell suspensions in the cultivar ‘Navolean’ is 3.0% settled cell volume. In the histo-differentiation phase the greatest formation of somatic embryos in the globular stage was obtained using a density of 12.0% final cell volume in liquid culture medium. The maturation of the embryos and an increase in germination was possible on using 0.5 gFW of somatic embryos during 30 days in the maturation culture medium. Using temporary immersion systems with 0.5 gFW of mature somatic embryos, the germination value was increased to 77.40% Key words: Musa, settled cell volume (SCV), somatic embryogenesis, temporary immersion
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spelling doaj.art-44fdb928d5da4feda59cab3c4dbd0cbd2022-12-22T01:40:48ZengUniversidad Central Marta Abreu de Las VillasBiotecnología Vegetal1609-18412074-86472002-04-0122132Multiplication, histodifferentiation and regeneration of embryogenic cell suspensión on the plantain cultivar ‘Navolean’ (AAB)Manuel CabreraJorge LópezRafael Gómez-KoskyNery MontanoMaritza ReyesD. ReinaldoJosé de la C. VenturaVictor MederoArletys SantosM. GarcíaMilagros BasailE. EspinosaThe results obtained show that the best cell density for the multiplication of the cell suspensions in the cultivar ‘Navolean’ is 3.0% settled cell volume. In the histo-differentiation phase the greatest formation of somatic embryos in the globular stage was obtained using a density of 12.0% final cell volume in liquid culture medium. The maturation of the embryos and an increase in germination was possible on using 0.5 gFW of somatic embryos during 30 days in the maturation culture medium. Using temporary immersion systems with 0.5 gFW of mature somatic embryos, the germination value was increased to 77.40% Key words: Musa, settled cell volume (SCV), somatic embryogenesis, temporary immersionhttps://revista.ibp.co.cu/index.php/BV/article/view/155
spellingShingle Manuel Cabrera
Jorge López
Rafael Gómez-Kosky
Nery Montano
Maritza Reyes
D. Reinaldo
José de la C. Ventura
Victor Medero
Arletys Santos
M. García
Milagros Basail
E. Espinosa
Multiplication, histodifferentiation and regeneration of embryogenic cell suspensión on the plantain cultivar ‘Navolean’ (AAB)
Biotecnología Vegetal
title Multiplication, histodifferentiation and regeneration of embryogenic cell suspensión on the plantain cultivar ‘Navolean’ (AAB)
title_full Multiplication, histodifferentiation and regeneration of embryogenic cell suspensión on the plantain cultivar ‘Navolean’ (AAB)
title_fullStr Multiplication, histodifferentiation and regeneration of embryogenic cell suspensión on the plantain cultivar ‘Navolean’ (AAB)
title_full_unstemmed Multiplication, histodifferentiation and regeneration of embryogenic cell suspensión on the plantain cultivar ‘Navolean’ (AAB)
title_short Multiplication, histodifferentiation and regeneration of embryogenic cell suspensión on the plantain cultivar ‘Navolean’ (AAB)
title_sort multiplication histodifferentiation and regeneration of embryogenic cell suspension on the plantain cultivar navolean aab
url https://revista.ibp.co.cu/index.php/BV/article/view/155
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