Determination of Prevalence and Risk Factors of Infection with Babesia ovis in Small Ruminants from West Azerbaijan Province, Iran by Polymerase Chain Reaction
Background: Small ruminants’ babesiosis caused by Babesia ovis, is transmitted during blood feeding by infected ticks and is the most economically important tick-borne disease in tropical and subtropical areas. This study was carried out to to estimate the infection rate of B. ovis in sheep and goat...
| Main Authors: | , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Tehran University of Medical Sciences
2015-10-01
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| Series: | Journal of Arthropod-Borne Diseases |
| Subjects: | |
| Online Access: | https://jad.tums.ac.ir/index.php/jad/article/view/136 |
| _version_ | 1818211540000571392 |
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| author | Bijan Esmaeilnejad Mousa Tavassoli Siamak Asri-Rezaei Bahram Dalir-Naghadeh Karim Mardani Mostafa Golabi Jafar Arjmand Ali Kazemnia Ghader Jalilzadeh |
| author_facet | Bijan Esmaeilnejad Mousa Tavassoli Siamak Asri-Rezaei Bahram Dalir-Naghadeh Karim Mardani Mostafa Golabi Jafar Arjmand Ali Kazemnia Ghader Jalilzadeh |
| author_sort | Bijan Esmaeilnejad |
| collection | DOAJ |
| description | Background: Small ruminants’ babesiosis caused by Babesia ovis, is transmitted during blood feeding by infected ticks and is the most economically important tick-borne disease in tropical and subtropical areas. This study was carried out to to estimate the infection rate of B. ovis in sheep and goats by PCR. We have analysed risk factors that might influence infection of sheep and goats with B. ovis.
Methods: A total 402 blood samples were examined microscopically for the presence of Babesia infection. All samples were tested by PCR. During sampling, whole body of each animal and farm dogs was examined for the presence of ticks.
Results: Forty-two animals (10.4%) were positive for Babesia spp. upon microscopic examination, whereas 67 animals (16.7%) yielded the specific DNA for B. ovis of which 52 animals were sheep and 15 animals were goats.
Twenty-nine farms (72.5%) were found positive for B. ovis. The percentage of positive animals in each location varied from 13 % to 20 %. The relative risk of the presence of ticks in sheep and goats (P< 0.01) and farm dogs (P< 0.01) for PCRpositive results forB. ovis in sheep and goats was found 3.8 and 2.9, respectively. A total of 747 ticks identified as Rhipicephalus bursa, R. sanguineus and R. turanicus on the basis of morphological features.
Conclusion: Other animal species besides dogs may also be risk factors for babesiosis in sheep and goats. Also, R. bursa may play an important role as a vector of the parasite in Iran. |
| first_indexed | 2024-12-12T05:34:07Z |
| format | Article |
| id | doaj.art-450395e5922e4d6381d6b433314658e3 |
| institution | Directory Open Access Journal |
| issn | 1735-7179 2322-2271 |
| language | English |
| last_indexed | 2024-12-12T05:34:07Z |
| publishDate | 2015-10-01 |
| publisher | Tehran University of Medical Sciences |
| record_format | Article |
| series | Journal of Arthropod-Borne Diseases |
| spelling | doaj.art-450395e5922e4d6381d6b433314658e32022-12-22T00:36:12ZengTehran University of Medical SciencesJournal of Arthropod-Borne Diseases1735-71792322-22712015-10-0192124Determination of Prevalence and Risk Factors of Infection with Babesia ovis in Small Ruminants from West Azerbaijan Province, Iran by Polymerase Chain ReactionBijan Esmaeilnejad0Mousa Tavassoli1Siamak Asri-Rezaei2Bahram Dalir-Naghadeh3Karim Mardani4Mostafa Golabi5Jafar Arjmand6Ali Kazemnia7Ghader Jalilzadeh8Department of Pathobiology, Faculty of Veterinary Medicine, Urmia University, Urmia, Iran.Department of Pathobiology, Faculty of Veterinary Medicine, Urmia University, Urmia, Iran.Departments of Clinical Sciences, Faculty of Veterinary Medicine, Urmia University, Urmia, Iran.Departments of Clinical Sciences, Faculty of Veterinary Medicine, Urmia University, Urmia, Iran.Department of Food Hygiene and Quality Control, Faculty of Veterinary Medicine, Urmia University, Urmia, Iran.Department of Pathobiology, Faculty of Veterinary Medicine, Urmia University, Urmia, Iran.Department of Pathobiology, Faculty of Veterinary Medicine, Urmia University, Urmia, Iran.Departments of Microbiology, Faculty of Veterinary Medicine, Urmia University, Urmia, Iran.Departments of Clinical Sciences, Faculty of Veterinary Medicine, Urmia University, Urmia, Iran.Background: Small ruminants’ babesiosis caused by Babesia ovis, is transmitted during blood feeding by infected ticks and is the most economically important tick-borne disease in tropical and subtropical areas. This study was carried out to to estimate the infection rate of B. ovis in sheep and goats by PCR. We have analysed risk factors that might influence infection of sheep and goats with B. ovis. Methods: A total 402 blood samples were examined microscopically for the presence of Babesia infection. All samples were tested by PCR. During sampling, whole body of each animal and farm dogs was examined for the presence of ticks. Results: Forty-two animals (10.4%) were positive for Babesia spp. upon microscopic examination, whereas 67 animals (16.7%) yielded the specific DNA for B. ovis of which 52 animals were sheep and 15 animals were goats. Twenty-nine farms (72.5%) were found positive for B. ovis. The percentage of positive animals in each location varied from 13 % to 20 %. The relative risk of the presence of ticks in sheep and goats (P< 0.01) and farm dogs (P< 0.01) for PCRpositive results forB. ovis in sheep and goats was found 3.8 and 2.9, respectively. A total of 747 ticks identified as Rhipicephalus bursa, R. sanguineus and R. turanicus on the basis of morphological features. Conclusion: Other animal species besides dogs may also be risk factors for babesiosis in sheep and goats. Also, R. bursa may play an important role as a vector of the parasite in Iran.https://jad.tums.ac.ir/index.php/jad/article/view/136BabesiaRhipicephalus Small ruminantIranPCR |
| spellingShingle | Bijan Esmaeilnejad Mousa Tavassoli Siamak Asri-Rezaei Bahram Dalir-Naghadeh Karim Mardani Mostafa Golabi Jafar Arjmand Ali Kazemnia Ghader Jalilzadeh Determination of Prevalence and Risk Factors of Infection with Babesia ovis in Small Ruminants from West Azerbaijan Province, Iran by Polymerase Chain Reaction Journal of Arthropod-Borne Diseases Babesia Rhipicephalus Small ruminant Iran PCR |
| title | Determination of Prevalence and Risk Factors of Infection with Babesia ovis in Small Ruminants from West Azerbaijan Province, Iran by Polymerase Chain Reaction |
| title_full | Determination of Prevalence and Risk Factors of Infection with Babesia ovis in Small Ruminants from West Azerbaijan Province, Iran by Polymerase Chain Reaction |
| title_fullStr | Determination of Prevalence and Risk Factors of Infection with Babesia ovis in Small Ruminants from West Azerbaijan Province, Iran by Polymerase Chain Reaction |
| title_full_unstemmed | Determination of Prevalence and Risk Factors of Infection with Babesia ovis in Small Ruminants from West Azerbaijan Province, Iran by Polymerase Chain Reaction |
| title_short | Determination of Prevalence and Risk Factors of Infection with Babesia ovis in Small Ruminants from West Azerbaijan Province, Iran by Polymerase Chain Reaction |
| title_sort | determination of prevalence and risk factors of infection with babesia ovis in small ruminants from west azerbaijan province iran by polymerase chain reaction |
| topic | Babesia Rhipicephalus Small ruminant Iran PCR |
| url | https://jad.tums.ac.ir/index.php/jad/article/view/136 |
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