Mutation in ribosomal protein S5 leads to spectinomycin resistance in Neisseria gonorrhoeae.
Spectinomycin remains a useful reserve option for therapy of gonorrhea. The emergence of multidrug-resistant Neisseria gonorrhoeae strains with decreased susceptibility to cefixime and to ceftriaxone makes it the only medicine still effective for treatment of gonorrhea infection in analogous cases....
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2013-07-01
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| Series: | Frontiers in Microbiology |
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| Online Access: | http://journal.frontiersin.org/Journal/10.3389/fmicb.2013.00186/full |
| _version_ | 1818365762264367104 |
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| author | Elena eIlina Maja eMalakhova Ivan eBodoev Alla eFilimonova Nina eOparina Vadim eGovorun |
| author_facet | Elena eIlina Maja eMalakhova Ivan eBodoev Alla eFilimonova Nina eOparina Vadim eGovorun |
| author_sort | Elena eIlina |
| collection | DOAJ |
| description | Spectinomycin remains a useful reserve option for therapy of gonorrhea. The emergence of multidrug-resistant Neisseria gonorrhoeae strains with decreased susceptibility to cefixime and to ceftriaxone makes it the only medicine still effective for treatment of gonorrhea infection in analogous cases. However, adoption of spectinomycin as a routinely used drug of choice was soon followed by reports of spectinomycin resistance. The main molecular mechanism of spectinomycin resistance in N. gonorrhoeae was C1192T substitution in 16S rRNA genes. Here we reported a Thr-24→Pro mutation in ribosomal protein S5 found in spectinomycin resistant clinical N. gonorrhoeae strain, which carried no changes in 16S rRNA. In a series of experiments, the transfer of rpsE gene allele encoding the mutant ribosomal protein S5 to the recipient N. gonorrhoeae strains was analyzed. The relatively high rate of transformation (ca. 10-5 CFUs) indicates the possibility of spread of spectinonycin resistance within gonococcal population due to the horizontal gene transfer. |
| first_indexed | 2024-12-13T22:25:25Z |
| format | Article |
| id | doaj.art-450470c3c1ad4f8fac030c365eac6288 |
| institution | Directory Open Access Journal |
| issn | 1664-302X |
| language | English |
| last_indexed | 2024-12-13T22:25:25Z |
| publishDate | 2013-07-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Microbiology |
| spelling | doaj.art-450470c3c1ad4f8fac030c365eac62882022-12-21T23:29:14ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2013-07-01410.3389/fmicb.2013.0018651849Mutation in ribosomal protein S5 leads to spectinomycin resistance in Neisseria gonorrhoeae.Elena eIlina0Maja eMalakhova1Ivan eBodoev2Alla eFilimonova3Nina eOparina4Vadim eGovorun5Research Institute for Physical Chemical MedicineResearch Institute for Physical Chemical MedicineResearch Institute for Physical Chemical MedicineResearch Institute for Physical Chemical MedicineResearch Institute for Physical Chemical MedicineResearch Institute for Physical Chemical MedicineSpectinomycin remains a useful reserve option for therapy of gonorrhea. The emergence of multidrug-resistant Neisseria gonorrhoeae strains with decreased susceptibility to cefixime and to ceftriaxone makes it the only medicine still effective for treatment of gonorrhea infection in analogous cases. However, adoption of spectinomycin as a routinely used drug of choice was soon followed by reports of spectinomycin resistance. The main molecular mechanism of spectinomycin resistance in N. gonorrhoeae was C1192T substitution in 16S rRNA genes. Here we reported a Thr-24→Pro mutation in ribosomal protein S5 found in spectinomycin resistant clinical N. gonorrhoeae strain, which carried no changes in 16S rRNA. In a series of experiments, the transfer of rpsE gene allele encoding the mutant ribosomal protein S5 to the recipient N. gonorrhoeae strains was analyzed. The relatively high rate of transformation (ca. 10-5 CFUs) indicates the possibility of spread of spectinonycin resistance within gonococcal population due to the horizontal gene transfer.http://journal.frontiersin.org/Journal/10.3389/fmicb.2013.00186/fullRibosomal ProteinsSpectinomycinmutationsantimicrobial resistancehorizontal gene transferSpot-transformation |
| spellingShingle | Elena eIlina Maja eMalakhova Ivan eBodoev Alla eFilimonova Nina eOparina Vadim eGovorun Mutation in ribosomal protein S5 leads to spectinomycin resistance in Neisseria gonorrhoeae. Frontiers in Microbiology Ribosomal Proteins Spectinomycin mutations antimicrobial resistance horizontal gene transfer Spot-transformation |
| title | Mutation in ribosomal protein S5 leads to spectinomycin resistance in Neisseria gonorrhoeae. |
| title_full | Mutation in ribosomal protein S5 leads to spectinomycin resistance in Neisseria gonorrhoeae. |
| title_fullStr | Mutation in ribosomal protein S5 leads to spectinomycin resistance in Neisseria gonorrhoeae. |
| title_full_unstemmed | Mutation in ribosomal protein S5 leads to spectinomycin resistance in Neisseria gonorrhoeae. |
| title_short | Mutation in ribosomal protein S5 leads to spectinomycin resistance in Neisseria gonorrhoeae. |
| title_sort | mutation in ribosomal protein s5 leads to spectinomycin resistance in neisseria gonorrhoeae |
| topic | Ribosomal Proteins Spectinomycin mutations antimicrobial resistance horizontal gene transfer Spot-transformation |
| url | http://journal.frontiersin.org/Journal/10.3389/fmicb.2013.00186/full |
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