Repressive mutations restore function-loss caused by the disruption of trimerization in Escherichia coli multidrug transporter AcrB

Repressive mutations restore function-loss caused by the disruption of trimerization in Escherichia coli multidrug transporter AcrB

AcrAB-TolC and their homologs are major multidrug efflux systems in Gram-negative bacteria. The inner membrane component AcrB functions as a trimer. Replacement of Pro223 by Gly in AcrB decreases the trimer stability and drastically reduces the drug efflux activity. The goal of this study is to iden...

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Bibliographic Details
Main Authors: Zhaoshuai eWang, Meng eZhong, Wei eLu, Qian eChai, Yinan eWei
Format: Article
Language:English
Published: Frontiers Media S.A. 2015-01-01
Series:Frontiers in Microbiology
Subjects:
Oligomerization
efflux pump
membrane transporter
RND
protein thermal stability
Online Access:http://journal.frontiersin.org/Journal/10.3389/fmicb.2015.00004/full

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