Blue light stimulates light stress and phototactic behavior when received in the brain of Diaphorina citri
Blue light with a wavelength of 400–470 nm is the composition of the visible light. However, in recent years, blue light contributed the most significance to light pollution due to the artificial light at night. Previously, we have demonstrated that the Asian citrus psyllid (ACP), Diaphorina citri,...
| Main Authors: | , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2023-02-01
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| Series: | Ecotoxicology and Environmental Safety |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S0147651323000234 |
| _version_ | 1797936443847344128 |
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| author | Fei-Feng Wang Ming-Hui Wang Meng-Ke Zhang Peng Qin Andrew G.S. Cuthbertson Chao-Liang Lei Bao-Li Qiu Lin Yu Wen Sang |
| author_facet | Fei-Feng Wang Ming-Hui Wang Meng-Ke Zhang Peng Qin Andrew G.S. Cuthbertson Chao-Liang Lei Bao-Li Qiu Lin Yu Wen Sang |
| author_sort | Fei-Feng Wang |
| collection | DOAJ |
| description | Blue light with a wavelength of 400–470 nm is the composition of the visible light. However, in recent years, blue light contributed the most significance to light pollution due to the artificial light at night. Previously, we have demonstrated that the Asian citrus psyllid (ACP), Diaphorina citri, an important pest in citrus production, has significant positive phototaxis with a light-emitting diode light of 400 nm. In this study, ACP with positive phototactic behavior to 400 nm light (PH) and non-phototactic behavior to 400 nm light (NP) were collected, individually. Transcriptome dynamics of head tissues of PH and NP groups were captured by using RNA-sequencing technology, respectively. Forty-three to 46 million clean reads with high-quality values were obtained, and 1773 differential expressed genes (DEGs) were detected. Compared with the NP group, there were 841 up-regulated DEGs and 932 down-regulated DEGs in the PH group. Eight pathways were significantly enriched in the PH group in the KEGG database, while 43 up-regulated pathways and 25 down-regulated pathways were significantly enriched in the PH group in the GO database. The DGE approach was reliable validated by real time quantitative PCR. Results indicated that the blue light acted as an abiotic stress causing physiological and biochemical responses such as oxidative stress, protein denaturation, inflammation and tumor development in ACPs. Additionally, the light was absorbed by photoreceptors of ACPs, and converted into electrical signal to regulate neuromodulation. This study provides basic information for understanding the molecular mechanisms of ACP in response to blue light and provides a reference for further studies to elucidate phototactic behavior. |
| first_indexed | 2024-04-10T18:29:44Z |
| format | Article |
| id | doaj.art-450b110d6e7544c39cc73b470a170478 |
| institution | Directory Open Access Journal |
| issn | 0147-6513 |
| language | English |
| last_indexed | 2024-04-10T18:29:44Z |
| publishDate | 2023-02-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Ecotoxicology and Environmental Safety |
| spelling | doaj.art-450b110d6e7544c39cc73b470a1704782023-02-02T04:46:54ZengElsevierEcotoxicology and Environmental Safety0147-65132023-02-01251114519Blue light stimulates light stress and phototactic behavior when received in the brain of Diaphorina citriFei-Feng Wang0Ming-Hui Wang1Meng-Ke Zhang2Peng Qin3Andrew G.S. Cuthbertson4Chao-Liang Lei5Bao-Li Qiu6Lin Yu7Wen Sang8South China Agricultural University, Guangzhou 510640, ChinaSouth China Agricultural University, Guangzhou 510640, China; Guangdong Laboratory for Lingnan Modern Agriculture, Guangzhou 510640, ChinaSouth China Agricultural University, Guangzhou 510640, ChinaSouth China Agricultural University, Guangzhou 510640, ChinaIndependent Science Advisor, York YO10 5AQ, United KingdomHubei Insect Resources Utilization and Sustainable Pest Management Key Laboratory, Huazhong Agricultural University, Wuhan 430070, ChinaSouth China Agricultural University, Guangzhou 510640, China; Guangdong Laboratory for Lingnan Modern Agriculture, Guangzhou 510640, ChinaInstitute of Plant Protection, Guangdong Academy of Agricultural Sciences, Key Laboratory of Green Prevention and Control on Fruits and Vegetables in South China Ministry of Agriculture and Rural Affairs, Guangdong Provincial Key Laboratory of High Technology for Plant Protection, Guangzhou 510640, China; Corresponding author.South China Agricultural University, Guangzhou 510640, China; Guangdong Laboratory for Lingnan Modern Agriculture, Guangzhou 510640, China; Corresponding author at: South China Agricultural University, Guangzhou 510640, China.Blue light with a wavelength of 400–470 nm is the composition of the visible light. However, in recent years, blue light contributed the most significance to light pollution due to the artificial light at night. Previously, we have demonstrated that the Asian citrus psyllid (ACP), Diaphorina citri, an important pest in citrus production, has significant positive phototaxis with a light-emitting diode light of 400 nm. In this study, ACP with positive phototactic behavior to 400 nm light (PH) and non-phototactic behavior to 400 nm light (NP) were collected, individually. Transcriptome dynamics of head tissues of PH and NP groups were captured by using RNA-sequencing technology, respectively. Forty-three to 46 million clean reads with high-quality values were obtained, and 1773 differential expressed genes (DEGs) were detected. Compared with the NP group, there were 841 up-regulated DEGs and 932 down-regulated DEGs in the PH group. Eight pathways were significantly enriched in the PH group in the KEGG database, while 43 up-regulated pathways and 25 down-regulated pathways were significantly enriched in the PH group in the GO database. The DGE approach was reliable validated by real time quantitative PCR. Results indicated that the blue light acted as an abiotic stress causing physiological and biochemical responses such as oxidative stress, protein denaturation, inflammation and tumor development in ACPs. Additionally, the light was absorbed by photoreceptors of ACPs, and converted into electrical signal to regulate neuromodulation. This study provides basic information for understanding the molecular mechanisms of ACP in response to blue light and provides a reference for further studies to elucidate phototactic behavior.http://www.sciencedirect.com/science/article/pii/S0147651323000234Asian citrus psyllidLight stressTranscriptomeOxidative stressProtein denaturationNeuromodulation |
| spellingShingle | Fei-Feng Wang Ming-Hui Wang Meng-Ke Zhang Peng Qin Andrew G.S. Cuthbertson Chao-Liang Lei Bao-Li Qiu Lin Yu Wen Sang Blue light stimulates light stress and phototactic behavior when received in the brain of Diaphorina citri Ecotoxicology and Environmental Safety Asian citrus psyllid Light stress Transcriptome Oxidative stress Protein denaturation Neuromodulation |
| title | Blue light stimulates light stress and phototactic behavior when received in the brain of Diaphorina citri |
| title_full | Blue light stimulates light stress and phototactic behavior when received in the brain of Diaphorina citri |
| title_fullStr | Blue light stimulates light stress and phototactic behavior when received in the brain of Diaphorina citri |
| title_full_unstemmed | Blue light stimulates light stress and phototactic behavior when received in the brain of Diaphorina citri |
| title_short | Blue light stimulates light stress and phototactic behavior when received in the brain of Diaphorina citri |
| title_sort | blue light stimulates light stress and phototactic behavior when received in the brain of diaphorina citri |
| topic | Asian citrus psyllid Light stress Transcriptome Oxidative stress Protein denaturation Neuromodulation |
| url | http://www.sciencedirect.com/science/article/pii/S0147651323000234 |
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