Comparison of Meropenem MIC by E Test and VITEK 2 in Resistant Pseudomonas and Acinetobacter Isolates
<p><em>P.aruginosa</em> and <em>A.baumannii</em> have evolved causing serious infections especially in health care institutions. Most of them are multidrug resistant and even resistant to meropenem which is a broad spectrum beta lactam antimicrobial used for treatment o...
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| Format: | Article |
| Language: | English |
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Sri Lankan Society for Microbiology
2012-01-01
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| Series: | Sri Lankan Journal of Infectious Diseases |
| Subjects: | |
| Online Access: | https://sljid.sljol.info/articles/3667 |
| _version_ | 1798022980802969600 |
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| author | Jananie Kottahachchi Joan Faoagali Sharon Kleinschmidt |
| author_facet | Jananie Kottahachchi Joan Faoagali Sharon Kleinschmidt |
| author_sort | Jananie Kottahachchi |
| collection | DOAJ |
| description | <p><em>P.aruginosa</em> and <em>A.baumannii</em> have evolved causing serious infections especially in health care institutions. Most of them are multidrug resistant and even resistant to meropenem which is a broad spectrum beta lactam antimicrobial used for treatment of critical infections. Minimum inhibitory concentration (MIC) of an antibiotic is determined to decide on the specific treatment and several methods of detecting MIC are adopted. Routing conduction of such methods is cumbersome for clinical laboratories and currently introduced VITEK 2 automated method is an alternative. The aims of the study were,</p><p>-To compare the E test and VITEK 2 system for the susceptibility testing of resistant <em>Pseudomonas aeruginosa </em>and <em>Acinetobacter baumannii </em>for meropenem and,</p><p>-To compare the effect of four carbapenem antibiotics on <em>Pseudomonas aeruginosa </em>and <em>Acinetobacter baumannii</em>. For that 75 <em>P. aeruginosa</em> and 25 <em>A. baumanii </em>were collected randomly from the collection of isolates at Princess Alexandra Hospital, Australia. E test and VITEK 2 MIC done for the each isolate according to the manufacturer’s instructions and the latest CLSI guidelines (June, 2010). VITEK-2 MICs corresponded closely with those obtained with the E test method. Categorical Agreement testing for both the organisms was 92% with no major errors and 08% minor error. We conclude that VITEK 2 is a reliable method to detect MIC in <em>P. aeruginosa </em>and<em> A. baumanii. </em>Meropenem MICs for <em>A. baumannii </em>with the VITEK 2 system usually follow pattern a very similar to <em>P. aeruginosa. </em>Doripenem sensitivity results can be extrapolated from meropenem. And ertapenem resistance to <em>P. aeruginosa </em>and<em> A. baumanii </em>is<em> </em> confirmed.</p><p>DOI: <a href="http://dx.doi.org/10.4038/sljid.v2i1.3667">http://dx.doi.org/10.4038/sljid.v2i1.3667</a></p><p><em>Sri Lankan Journal of Infectious Diseases </em>Vol.2(1) 2012: 28-35</p><p> </p><p> </p><p> </p> |
| first_indexed | 2024-04-11T17:38:45Z |
| format | Article |
| id | doaj.art-450f047fa6a5462fa60bad0e4d4857b7 |
| institution | Directory Open Access Journal |
| issn | 2012-8169 2448-9654 |
| language | English |
| last_indexed | 2024-04-11T17:38:45Z |
| publishDate | 2012-01-01 |
| publisher | Sri Lankan Society for Microbiology |
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| series | Sri Lankan Journal of Infectious Diseases |
| spelling | doaj.art-450f047fa6a5462fa60bad0e4d4857b72022-12-22T04:11:32ZengSri Lankan Society for MicrobiologySri Lankan Journal of Infectious Diseases2012-81692448-96542012-01-0121283510.4038/sljid.v2i1.36673372Comparison of Meropenem MIC by E Test and VITEK 2 in Resistant Pseudomonas and Acinetobacter IsolatesJananie Kottahachchi0Joan Faoagali1Sharon Kleinschmidt2Sri Jayewardenepura UniversityPrincess Alexandra HospitalPrincess Alexandra Hospital<p><em>P.aruginosa</em> and <em>A.baumannii</em> have evolved causing serious infections especially in health care institutions. Most of them are multidrug resistant and even resistant to meropenem which is a broad spectrum beta lactam antimicrobial used for treatment of critical infections. Minimum inhibitory concentration (MIC) of an antibiotic is determined to decide on the specific treatment and several methods of detecting MIC are adopted. Routing conduction of such methods is cumbersome for clinical laboratories and currently introduced VITEK 2 automated method is an alternative. The aims of the study were,</p><p>-To compare the E test and VITEK 2 system for the susceptibility testing of resistant <em>Pseudomonas aeruginosa </em>and <em>Acinetobacter baumannii </em>for meropenem and,</p><p>-To compare the effect of four carbapenem antibiotics on <em>Pseudomonas aeruginosa </em>and <em>Acinetobacter baumannii</em>. For that 75 <em>P. aeruginosa</em> and 25 <em>A. baumanii </em>were collected randomly from the collection of isolates at Princess Alexandra Hospital, Australia. E test and VITEK 2 MIC done for the each isolate according to the manufacturer’s instructions and the latest CLSI guidelines (June, 2010). VITEK-2 MICs corresponded closely with those obtained with the E test method. Categorical Agreement testing for both the organisms was 92% with no major errors and 08% minor error. We conclude that VITEK 2 is a reliable method to detect MIC in <em>P. aeruginosa </em>and<em> A. baumanii. </em>Meropenem MICs for <em>A. baumannii </em>with the VITEK 2 system usually follow pattern a very similar to <em>P. aeruginosa. </em>Doripenem sensitivity results can be extrapolated from meropenem. And ertapenem resistance to <em>P. aeruginosa </em>and<em> A. baumanii </em>is<em> </em> confirmed.</p><p>DOI: <a href="http://dx.doi.org/10.4038/sljid.v2i1.3667">http://dx.doi.org/10.4038/sljid.v2i1.3667</a></p><p><em>Sri Lankan Journal of Infectious Diseases </em>Vol.2(1) 2012: 28-35</p><p> </p><p> </p><p> </p>https://sljid.sljol.info/articles/3667meropenemmicvitek 2pseudomonas aeruginosaacinetobacter baumanii |
| spellingShingle | Jananie Kottahachchi Joan Faoagali Sharon Kleinschmidt Comparison of Meropenem MIC by E Test and VITEK 2 in Resistant Pseudomonas and Acinetobacter Isolates Sri Lankan Journal of Infectious Diseases meropenem mic vitek 2 pseudomonas aeruginosa acinetobacter baumanii |
| title | Comparison of Meropenem MIC by E Test and VITEK 2 in Resistant Pseudomonas and Acinetobacter Isolates |
| title_full | Comparison of Meropenem MIC by E Test and VITEK 2 in Resistant Pseudomonas and Acinetobacter Isolates |
| title_fullStr | Comparison of Meropenem MIC by E Test and VITEK 2 in Resistant Pseudomonas and Acinetobacter Isolates |
| title_full_unstemmed | Comparison of Meropenem MIC by E Test and VITEK 2 in Resistant Pseudomonas and Acinetobacter Isolates |
| title_short | Comparison of Meropenem MIC by E Test and VITEK 2 in Resistant Pseudomonas and Acinetobacter Isolates |
| title_sort | comparison of meropenem mic by e test and vitek 2 in resistant pseudomonas and acinetobacter isolates |
| topic | meropenem mic vitek 2 pseudomonas aeruginosa acinetobacter baumanii |
| url | https://sljid.sljol.info/articles/3667 |
| work_keys_str_mv | AT jananiekottahachchi comparisonofmeropenemmicbyetestandvitek2inresistantpseudomonasandacinetobacterisolates AT joanfaoagali comparisonofmeropenemmicbyetestandvitek2inresistantpseudomonasandacinetobacterisolates AT sharonkleinschmidt comparisonofmeropenemmicbyetestandvitek2inresistantpseudomonasandacinetobacterisolates |