ACSL1: A preliminary study that provides a new target for the treatment of renal fibrosis could bring new insights in diabetic kidney disease
Background: Renal fibrosis is the main cause of the development of diabetic kidney disease (DKD). ACSL1 plays an important role in colon cancer and liver fibrosis. Methods: We screened ACSL1 by proteomics analysis and then verified the expression of ACSL1 in the urine of diabetic nephropathy patient...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | Spanish |
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Elsevier
2023-12-01
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| Series: | Nefrología |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S0211699523000358 |
| _version_ | 1797390541627850752 |
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| author | Zhonghui Cao Xiao Gao Jing Meng Xiaoli Guo Jiahao Xu Junchao Cui Xueyan Zhou |
| author_facet | Zhonghui Cao Xiao Gao Jing Meng Xiaoli Guo Jiahao Xu Junchao Cui Xueyan Zhou |
| author_sort | Zhonghui Cao |
| collection | DOAJ |
| description | Background: Renal fibrosis is the main cause of the development of diabetic kidney disease (DKD). ACSL1 plays an important role in colon cancer and liver fibrosis. Methods: We screened ACSL1 by proteomics analysis and then verified the expression of ACSL1 in the urine of diabetic nephropathy patients by WB and ELISA. Then, a total of 12 db/m and db/db mice were used to verify the association between renal fibrosis and ACSL1. Periodic acid-Schiff (PAS) staining, Masson staining, and immunostaining were performed for histological studies. The relationship between ACSL1 and renal fibrosis was studied by knocking down ACSL1 in cell experiments. Results: The expression of ACSL1 was significantly increased in the exfoliated urine cells and urine supernatant of diabetic nephropathy patients and was closely related to renal function. In addition, the expression of ACSL1 was significantly increased in the renal tissues of db/db mice with fibrosis. Knocking down ACSL1 in HK-2 cells was shown to reverse renal fibrosis induced by high glucose. Conclusions: We found a potential therapeutic target for preventing or ameliorating the progression of DKD fibrosis. Reducing ACSL1 expression may be a new strategy for the treatment of renal fibrosis caused by DKD, which provides an experimental theoretical basis for new drug research. Resumen: Antecedentes: La fibrosis renal es la causa principal de desarrollo de nefropatía diabética (ND). ACSL1 juega un papel importante en el cáncer de colon y la fibrosis hepática. Métodos: Cribamos ACSL1 mediante análisis proteómico, verificando seguidamente la expresión de ACSL1 en la orina de los pacientes con nefropatía diabética mediante WB y ELISA. A continuación, utilizamos un total de 12 ratones db/m y db/db para verificar la asociación entre fibrosis renal y ACSL1. Se realizaron tinciones PAS (Periodic acid-Schiff), de Masson e inmunotinción para los estudios histológicos. Estudiamos la relación entre ACSL1 y fibrosis renal aplicando la técnica de knockdown a ACSL1 en los experimentos celulares. Resultados: La expresión de ACSL1 se incrementó significativamente en las células de orina exfoliada y el sobrenadante de orina de los pacientes con nefropatía diabética y estuvo estrechamente relacionada con la función renal. Además, la expresión de ACSL1 se incrementó significativamente en los tejidos renales de los ratones db/db con fibrosis. La realización de knockdown a ACSL1 en las células HK-2 reflejó una reversión de la fibrosis renal inducida por la alta tasa de glucosa. Conclusiones: Encontramos un objetivo terapéutico potencial para prevenir o mejorar la progresión de la fibrosis en la ND. Reducir la expresión de ACSL1 puede suponer una nueva estrategia para el tratamiento de la fibrosis renal causada por la ND, lo cual aporta una base teórica experimental para la investigación de un nuevo fármaco. |
| first_indexed | 2024-03-08T23:13:19Z |
| format | Article |
| id | doaj.art-45127bfb28784761b0b00c48cfbde54d |
| institution | Directory Open Access Journal |
| issn | 0211-6995 |
| language | Spanish |
| last_indexed | 2024-03-08T23:13:19Z |
| publishDate | 2023-12-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Nefrología |
| spelling | doaj.art-45127bfb28784761b0b00c48cfbde54d2023-12-15T07:22:36ZspaElsevierNefrología0211-69952023-12-01433846ACSL1: A preliminary study that provides a new target for the treatment of renal fibrosis could bring new insights in diabetic kidney diseaseZhonghui Cao0Xiao Gao1Jing Meng2Xiaoli Guo3Jiahao Xu4Junchao Cui5Xueyan Zhou6Jiangsu Key Laboratory of New Drug Research and Clinical Pharmacy, College of Pharmacy, Xuzhou Medical University, Xuzhou 221004, China; Department of Pharmacy, Affiliated Hospital of Xuzhou Medical University, Xuzhou 221004, ChinaJiangsu Key Laboratory of New Drug Research and Clinical Pharmacy, College of Pharmacy, Xuzhou Medical University, Xuzhou 221004, ChinaJiangsu Key Laboratory of New Drug Research and Clinical Pharmacy, College of Pharmacy, Xuzhou Medical University, Xuzhou 221004, ChinaJiangsu Key Laboratory of New Drug Research and Clinical Pharmacy, College of Pharmacy, Xuzhou Medical University, Xuzhou 221004, ChinaJiangsu Key Laboratory of New Drug Research and Clinical Pharmacy, College of Pharmacy, Xuzhou Medical University, Xuzhou 221004, ChinaJiangsu Key Laboratory of New Drug Research and Clinical Pharmacy, College of Pharmacy, Xuzhou Medical University, Xuzhou 221004, ChinaJiangsu Key Laboratory of New Drug Research and Clinical Pharmacy, College of Pharmacy, Xuzhou Medical University, Xuzhou 221004, China; Corresponding author.Background: Renal fibrosis is the main cause of the development of diabetic kidney disease (DKD). ACSL1 plays an important role in colon cancer and liver fibrosis. Methods: We screened ACSL1 by proteomics analysis and then verified the expression of ACSL1 in the urine of diabetic nephropathy patients by WB and ELISA. Then, a total of 12 db/m and db/db mice were used to verify the association between renal fibrosis and ACSL1. Periodic acid-Schiff (PAS) staining, Masson staining, and immunostaining were performed for histological studies. The relationship between ACSL1 and renal fibrosis was studied by knocking down ACSL1 in cell experiments. Results: The expression of ACSL1 was significantly increased in the exfoliated urine cells and urine supernatant of diabetic nephropathy patients and was closely related to renal function. In addition, the expression of ACSL1 was significantly increased in the renal tissues of db/db mice with fibrosis. Knocking down ACSL1 in HK-2 cells was shown to reverse renal fibrosis induced by high glucose. Conclusions: We found a potential therapeutic target for preventing or ameliorating the progression of DKD fibrosis. Reducing ACSL1 expression may be a new strategy for the treatment of renal fibrosis caused by DKD, which provides an experimental theoretical basis for new drug research. Resumen: Antecedentes: La fibrosis renal es la causa principal de desarrollo de nefropatía diabética (ND). ACSL1 juega un papel importante en el cáncer de colon y la fibrosis hepática. Métodos: Cribamos ACSL1 mediante análisis proteómico, verificando seguidamente la expresión de ACSL1 en la orina de los pacientes con nefropatía diabética mediante WB y ELISA. A continuación, utilizamos un total de 12 ratones db/m y db/db para verificar la asociación entre fibrosis renal y ACSL1. Se realizaron tinciones PAS (Periodic acid-Schiff), de Masson e inmunotinción para los estudios histológicos. Estudiamos la relación entre ACSL1 y fibrosis renal aplicando la técnica de knockdown a ACSL1 en los experimentos celulares. Resultados: La expresión de ACSL1 se incrementó significativamente en las células de orina exfoliada y el sobrenadante de orina de los pacientes con nefropatía diabética y estuvo estrechamente relacionada con la función renal. Además, la expresión de ACSL1 se incrementó significativamente en los tejidos renales de los ratones db/db con fibrosis. La realización de knockdown a ACSL1 en las células HK-2 reflejó una reversión de la fibrosis renal inducida por la alta tasa de glucosa. Conclusiones: Encontramos un objetivo terapéutico potencial para prevenir o mejorar la progresión de la fibrosis en la ND. Reducir la expresión de ACSL1 puede suponer una nueva estrategia para el tratamiento de la fibrosis renal causada por la ND, lo cual aporta una base teórica experimental para la investigación de un nuevo fármaco.http://www.sciencedirect.com/science/article/pii/S0211699523000358ACSL1Nefropatía diabéticaFibrosis renal |
| spellingShingle | Zhonghui Cao Xiao Gao Jing Meng Xiaoli Guo Jiahao Xu Junchao Cui Xueyan Zhou ACSL1: A preliminary study that provides a new target for the treatment of renal fibrosis could bring new insights in diabetic kidney disease Nefrología ACSL1 Nefropatía diabética Fibrosis renal |
| title | ACSL1: A preliminary study that provides a new target for the treatment of renal fibrosis could bring new insights in diabetic kidney disease |
| title_full | ACSL1: A preliminary study that provides a new target for the treatment of renal fibrosis could bring new insights in diabetic kidney disease |
| title_fullStr | ACSL1: A preliminary study that provides a new target for the treatment of renal fibrosis could bring new insights in diabetic kidney disease |
| title_full_unstemmed | ACSL1: A preliminary study that provides a new target for the treatment of renal fibrosis could bring new insights in diabetic kidney disease |
| title_short | ACSL1: A preliminary study that provides a new target for the treatment of renal fibrosis could bring new insights in diabetic kidney disease |
| title_sort | acsl1 a preliminary study that provides a new target for the treatment of renal fibrosis could bring new insights in diabetic kidney disease |
| topic | ACSL1 Nefropatía diabética Fibrosis renal |
| url | http://www.sciencedirect.com/science/article/pii/S0211699523000358 |
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