Sulfane Sulfur Regulates LasR-Mediated Quorum Sensing and Virulence in <i>Pseudomonas aeruginosa</i> PAO1
Sulfane sulfur, such as inorganic and organic polysulfide (HS<sub>n</sub><sup>−</sup> and RS<sub>n</sub><sup>−</sup>, <i>n</i> > 2), is a common cellular component, produced either from hydrogen sulfide oxidation or cysteine metabolism....
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| Format: | Article |
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MDPI AG
2021-09-01
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| Series: | Antioxidants |
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| Online Access: | https://www.mdpi.com/2076-3921/10/9/1498 |
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| author | Guanhua Xuan Chuanjuan Lv Huangwei Xu Kai Li Huaiwei Liu Yongzhen Xia Luying Xun |
| author_facet | Guanhua Xuan Chuanjuan Lv Huangwei Xu Kai Li Huaiwei Liu Yongzhen Xia Luying Xun |
| author_sort | Guanhua Xuan |
| collection | DOAJ |
| description | Sulfane sulfur, such as inorganic and organic polysulfide (HS<sub>n</sub><sup>−</sup> and RS<sub>n</sub><sup>−</sup>, <i>n</i> > 2), is a common cellular component, produced either from hydrogen sulfide oxidation or cysteine metabolism. In <i>Pseudomonas aeruginosa</i> PAO1, LasR is a quorum sensing master regulator. After binding its autoinducer, LasR binds to its target DNA to activate the transcription of a suite of genes, including virulence factors. Herein, we report that the production of hydrogen sulfide and sulfane sulfur were positively correlated in <i>P. aeruginosa</i> PAO1, and sulfane sulfur was able to modify LasR, which generated Cys<sup>188</sup> persulfide and trisulfide and produced a pentasulfur link between Cys<sup>201</sup> and Cys<sup>203</sup>. The modifications did not affect LasR binding to its target DNA site, but made it several-fold more effective than unmodified LasR in activating transcription in both in vitro and in vivo assays. On the contrary, H<sub>2</sub>O<sub>2</sub> inactivates LasR via producing a disulfide bond between Cys<sup>201</sup> and Cys<sup>203</sup>. <i>P. aeruginosa</i> PAO1 had a high cellular sulfane sulfur and high LasR activity in the mid log phase and early stationary phase, but a low sulfane sulfur and low LasR activity in the declination phase. Thus, sulfane sulfur is a new signaling factor in the bacterium, adding another level of control over LasR-mediated quorum sensing and turning down the activity in old cells. |
| first_indexed | 2024-03-10T07:55:56Z |
| format | Article |
| id | doaj.art-4512f3b90caf4c8da12ef47ae3f37ff4 |
| institution | Directory Open Access Journal |
| issn | 2076-3921 |
| language | English |
| last_indexed | 2024-03-10T07:55:56Z |
| publishDate | 2021-09-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Antioxidants |
| spelling | doaj.art-4512f3b90caf4c8da12ef47ae3f37ff42023-11-22T11:49:44ZengMDPI AGAntioxidants2076-39212021-09-01109149810.3390/antiox10091498Sulfane Sulfur Regulates LasR-Mediated Quorum Sensing and Virulence in <i>Pseudomonas aeruginosa</i> PAO1Guanhua Xuan0Chuanjuan Lv1Huangwei Xu2Kai Li3Huaiwei Liu4Yongzhen Xia5Luying Xun6State Key Laboratory of Microbial Technology, Shandong University, 72 Binhai Road, Qingdao 266237, ChinaState Key Laboratory of Microbial Technology, Shandong University, 72 Binhai Road, Qingdao 266237, ChinaState Key Laboratory of Microbial Technology, Shandong University, 72 Binhai Road, Qingdao 266237, ChinaState Key Laboratory of Microbial Technology, Shandong University, 72 Binhai Road, Qingdao 266237, ChinaState Key Laboratory of Microbial Technology, Shandong University, 72 Binhai Road, Qingdao 266237, ChinaState Key Laboratory of Microbial Technology, Shandong University, 72 Binhai Road, Qingdao 266237, ChinaState Key Laboratory of Microbial Technology, Shandong University, 72 Binhai Road, Qingdao 266237, ChinaSulfane sulfur, such as inorganic and organic polysulfide (HS<sub>n</sub><sup>−</sup> and RS<sub>n</sub><sup>−</sup>, <i>n</i> > 2), is a common cellular component, produced either from hydrogen sulfide oxidation or cysteine metabolism. In <i>Pseudomonas aeruginosa</i> PAO1, LasR is a quorum sensing master regulator. After binding its autoinducer, LasR binds to its target DNA to activate the transcription of a suite of genes, including virulence factors. Herein, we report that the production of hydrogen sulfide and sulfane sulfur were positively correlated in <i>P. aeruginosa</i> PAO1, and sulfane sulfur was able to modify LasR, which generated Cys<sup>188</sup> persulfide and trisulfide and produced a pentasulfur link between Cys<sup>201</sup> and Cys<sup>203</sup>. The modifications did not affect LasR binding to its target DNA site, but made it several-fold more effective than unmodified LasR in activating transcription in both in vitro and in vivo assays. On the contrary, H<sub>2</sub>O<sub>2</sub> inactivates LasR via producing a disulfide bond between Cys<sup>201</sup> and Cys<sup>203</sup>. <i>P. aeruginosa</i> PAO1 had a high cellular sulfane sulfur and high LasR activity in the mid log phase and early stationary phase, but a low sulfane sulfur and low LasR activity in the declination phase. Thus, sulfane sulfur is a new signaling factor in the bacterium, adding another level of control over LasR-mediated quorum sensing and turning down the activity in old cells.https://www.mdpi.com/2076-3921/10/9/1498quorum sensingLasRsulfane sulfursignalingprotein persulfidationvirulence |
| spellingShingle | Guanhua Xuan Chuanjuan Lv Huangwei Xu Kai Li Huaiwei Liu Yongzhen Xia Luying Xun Sulfane Sulfur Regulates LasR-Mediated Quorum Sensing and Virulence in <i>Pseudomonas aeruginosa</i> PAO1 Antioxidants quorum sensing LasR sulfane sulfur signaling protein persulfidation virulence |
| title | Sulfane Sulfur Regulates LasR-Mediated Quorum Sensing and Virulence in <i>Pseudomonas aeruginosa</i> PAO1 |
| title_full | Sulfane Sulfur Regulates LasR-Mediated Quorum Sensing and Virulence in <i>Pseudomonas aeruginosa</i> PAO1 |
| title_fullStr | Sulfane Sulfur Regulates LasR-Mediated Quorum Sensing and Virulence in <i>Pseudomonas aeruginosa</i> PAO1 |
| title_full_unstemmed | Sulfane Sulfur Regulates LasR-Mediated Quorum Sensing and Virulence in <i>Pseudomonas aeruginosa</i> PAO1 |
| title_short | Sulfane Sulfur Regulates LasR-Mediated Quorum Sensing and Virulence in <i>Pseudomonas aeruginosa</i> PAO1 |
| title_sort | sulfane sulfur regulates lasr mediated quorum sensing and virulence in i pseudomonas aeruginosa i pao1 |
| topic | quorum sensing LasR sulfane sulfur signaling protein persulfidation virulence |
| url | https://www.mdpi.com/2076-3921/10/9/1498 |
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