Mechanism of Sugarbeet Seed Germination Enhanced by Hydrogen Peroxide
Seed germination is a critical first stage of plant development but can be arrested by factors including dormancy and environmental conditions. Strategies to enhance germination are of interest to plant breeders to ensure the ability to utilize the genetic potential residing inside a dormant seed. I...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2022-04-01
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| Series: | Frontiers in Plant Science |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fpls.2022.888519/full |
| _version_ | 1828350756828217344 |
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| author | Chenggen Chu Rachael Claire Poore Melvin D. Bolton Karen K. Fugate |
| author_facet | Chenggen Chu Rachael Claire Poore Melvin D. Bolton Karen K. Fugate |
| author_sort | Chenggen Chu |
| collection | DOAJ |
| description | Seed germination is a critical first stage of plant development but can be arrested by factors including dormancy and environmental conditions. Strategies to enhance germination are of interest to plant breeders to ensure the ability to utilize the genetic potential residing inside a dormant seed. In this study, seed germination in two sugarbeet (Beta vulgaris ssp. vulgaris L.) lines F1004 and F1015 through incubating seeds in hydrogen peroxide (H2O2) solution was improved over 70% relative to germinating seeds through water incubation. It was further found that low germination from water incubation was caused by physical dormancy in F1015 seeds with initial seed imbibition blocked by the seed pericarp, and physiological dormancy in F1004 seeds with germination compromised due to the physiological condition of the embryo. To identify genes that are differentially expressed in response to cellular activities promoted by H2O2 during overcoming different type of dormancies, an RNA-Seq study was carried out and found H2O2 treatment during germination accelerated the degradation of seed stored mRNAs that were synthesized before or during seed storage to provide protections and maintain the dormant state. Comparison of transcripts in H2O2-treated seeds between the two sugarbeet lines identified differentially expressed genes (DEGs) that were higher in F1004 for alleviating physiological dormancy were known to relative to gene expression regulation. The research established that H2O2 overcomes both physical and physiological dormancies by hastening the transition of seeds from dormancy into germination. More DEGs related to gene expression regulation were involved in relieving physiological dormancy which provides new knowledge about the role of exogenous H2O2 as a signaling molecule for regulating gene activities during germination. Moreover, the protocol using H2O2 to promote germination will be useful for rescuing plant germplasms with poor germination. |
| first_indexed | 2024-04-14T01:29:48Z |
| format | Article |
| id | doaj.art-45132477d0104840b006d5e2a4c17390 |
| institution | Directory Open Access Journal |
| issn | 1664-462X |
| language | English |
| last_indexed | 2024-04-14T01:29:48Z |
| publishDate | 2022-04-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Plant Science |
| spelling | doaj.art-45132477d0104840b006d5e2a4c173902022-12-22T02:20:13ZengFrontiers Media S.A.Frontiers in Plant Science1664-462X2022-04-011310.3389/fpls.2022.888519888519Mechanism of Sugarbeet Seed Germination Enhanced by Hydrogen PeroxideChenggen ChuRachael Claire PooreMelvin D. BoltonKaren K. FugateSeed germination is a critical first stage of plant development but can be arrested by factors including dormancy and environmental conditions. Strategies to enhance germination are of interest to plant breeders to ensure the ability to utilize the genetic potential residing inside a dormant seed. In this study, seed germination in two sugarbeet (Beta vulgaris ssp. vulgaris L.) lines F1004 and F1015 through incubating seeds in hydrogen peroxide (H2O2) solution was improved over 70% relative to germinating seeds through water incubation. It was further found that low germination from water incubation was caused by physical dormancy in F1015 seeds with initial seed imbibition blocked by the seed pericarp, and physiological dormancy in F1004 seeds with germination compromised due to the physiological condition of the embryo. To identify genes that are differentially expressed in response to cellular activities promoted by H2O2 during overcoming different type of dormancies, an RNA-Seq study was carried out and found H2O2 treatment during germination accelerated the degradation of seed stored mRNAs that were synthesized before or during seed storage to provide protections and maintain the dormant state. Comparison of transcripts in H2O2-treated seeds between the two sugarbeet lines identified differentially expressed genes (DEGs) that were higher in F1004 for alleviating physiological dormancy were known to relative to gene expression regulation. The research established that H2O2 overcomes both physical and physiological dormancies by hastening the transition of seeds from dormancy into germination. More DEGs related to gene expression regulation were involved in relieving physiological dormancy which provides new knowledge about the role of exogenous H2O2 as a signaling molecule for regulating gene activities during germination. Moreover, the protocol using H2O2 to promote germination will be useful for rescuing plant germplasms with poor germination.https://www.frontiersin.org/articles/10.3389/fpls.2022.888519/fullsugar beetseed dormancyRNA-Seqgene expressiondifferentially expressed genetranscript analysis |
| spellingShingle | Chenggen Chu Rachael Claire Poore Melvin D. Bolton Karen K. Fugate Mechanism of Sugarbeet Seed Germination Enhanced by Hydrogen Peroxide Frontiers in Plant Science sugar beet seed dormancy RNA-Seq gene expression differentially expressed gene transcript analysis |
| title | Mechanism of Sugarbeet Seed Germination Enhanced by Hydrogen Peroxide |
| title_full | Mechanism of Sugarbeet Seed Germination Enhanced by Hydrogen Peroxide |
| title_fullStr | Mechanism of Sugarbeet Seed Germination Enhanced by Hydrogen Peroxide |
| title_full_unstemmed | Mechanism of Sugarbeet Seed Germination Enhanced by Hydrogen Peroxide |
| title_short | Mechanism of Sugarbeet Seed Germination Enhanced by Hydrogen Peroxide |
| title_sort | mechanism of sugarbeet seed germination enhanced by hydrogen peroxide |
| topic | sugar beet seed dormancy RNA-Seq gene expression differentially expressed gene transcript analysis |
| url | https://www.frontiersin.org/articles/10.3389/fpls.2022.888519/full |
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