Potassium Ion Channel Gene OsAKT1 Affects Iron Translocation in Rice Plants Exposed to Iron Toxicity
Iron toxicity is one of the most widely spread mineral disorders in anaerobic soils, but the tolerance mechanisms in plants are poorly understood. Here we characterize the involvement of a rice potassium ion channel gene, OsAKT1, in Fe toxic conditions. Two knock-down lines of OsAKT1 together with a...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2019-05-01
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| Series: | Frontiers in Plant Science |
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| Online Access: | https://www.frontiersin.org/article/10.3389/fpls.2019.00579/full |
| _version_ | 1819139432170651648 |
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| author | Lin-Bo Wu Felix Holtkamp Andriele Wairich Andriele Wairich Michael Frei |
| author_facet | Lin-Bo Wu Felix Holtkamp Andriele Wairich Andriele Wairich Michael Frei |
| author_sort | Lin-Bo Wu |
| collection | DOAJ |
| description | Iron toxicity is one of the most widely spread mineral disorders in anaerobic soils, but the tolerance mechanisms in plants are poorly understood. Here we characterize the involvement of a rice potassium ion channel gene, OsAKT1, in Fe toxic conditions. Two knock-down lines of OsAKT1 together with azygos lines were investigated. Mutant lines did not differ from azygos lines regarding plant growth, gas exchange rate or chlorophyll fluorescence in control conditions. However, loss-of-function of OsAKT1 increased the sensitivity to excess Fe regarding leaf bronzing symptoms, reactive oxygen species generation, leaf spectral reflectance indices, and chlorophyll fluorescence. Fe toxicity leads to largely reduced uptake of other nutrients into shoots, which illustrates the complexity of Fe stress related to multiple mineral disorders. Less potassium uptake in the mutants compared to azygos lines co-occurred with higher amounts of Fe accumulated in the shoot tissues but not in the roots. These results were consistent with a higher level of Fe loaded into the xylem sap of mutants compared to azygos lines in the early phase of Fe toxicity. In conclusion, OsAKT1 is crucial for the tolerance of rice against Fe toxicity as K homeostasis affects Fe translocation from root to shoot. |
| first_indexed | 2024-12-22T11:22:34Z |
| format | Article |
| id | doaj.art-45156e367d614f10999f73d669b923c3 |
| institution | Directory Open Access Journal |
| issn | 1664-462X |
| language | English |
| last_indexed | 2024-12-22T11:22:34Z |
| publishDate | 2019-05-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Plant Science |
| spelling | doaj.art-45156e367d614f10999f73d669b923c32022-12-21T18:27:50ZengFrontiers Media S.A.Frontiers in Plant Science1664-462X2019-05-011010.3389/fpls.2019.00579449351Potassium Ion Channel Gene OsAKT1 Affects Iron Translocation in Rice Plants Exposed to Iron ToxicityLin-Bo Wu0Felix Holtkamp1Andriele Wairich2Andriele Wairich3Michael Frei4Department of Plant Nutrition, Institute of Crop Science and Resource Conservation, University of Bonn, Bonn, GermanyDepartment of Plant Nutrition, Institute of Crop Science and Resource Conservation, University of Bonn, Bonn, GermanyDepartment of Plant Nutrition, Institute of Crop Science and Resource Conservation, University of Bonn, Bonn, GermanyCenter for Biotechnology, Federal University of Rio Grande do Sul (UFRGS), Porto Alegre, BrazilDepartment of Plant Nutrition, Institute of Crop Science and Resource Conservation, University of Bonn, Bonn, GermanyIron toxicity is one of the most widely spread mineral disorders in anaerobic soils, but the tolerance mechanisms in plants are poorly understood. Here we characterize the involvement of a rice potassium ion channel gene, OsAKT1, in Fe toxic conditions. Two knock-down lines of OsAKT1 together with azygos lines were investigated. Mutant lines did not differ from azygos lines regarding plant growth, gas exchange rate or chlorophyll fluorescence in control conditions. However, loss-of-function of OsAKT1 increased the sensitivity to excess Fe regarding leaf bronzing symptoms, reactive oxygen species generation, leaf spectral reflectance indices, and chlorophyll fluorescence. Fe toxicity leads to largely reduced uptake of other nutrients into shoots, which illustrates the complexity of Fe stress related to multiple mineral disorders. Less potassium uptake in the mutants compared to azygos lines co-occurred with higher amounts of Fe accumulated in the shoot tissues but not in the roots. These results were consistent with a higher level of Fe loaded into the xylem sap of mutants compared to azygos lines in the early phase of Fe toxicity. In conclusion, OsAKT1 is crucial for the tolerance of rice against Fe toxicity as K homeostasis affects Fe translocation from root to shoot.https://www.frontiersin.org/article/10.3389/fpls.2019.00579/fullabiotic stressiron toxicitypotassiumpotassium ion channelricetolerance |
| spellingShingle | Lin-Bo Wu Felix Holtkamp Andriele Wairich Andriele Wairich Michael Frei Potassium Ion Channel Gene OsAKT1 Affects Iron Translocation in Rice Plants Exposed to Iron Toxicity Frontiers in Plant Science abiotic stress iron toxicity potassium potassium ion channel rice tolerance |
| title | Potassium Ion Channel Gene OsAKT1 Affects Iron Translocation in Rice Plants Exposed to Iron Toxicity |
| title_full | Potassium Ion Channel Gene OsAKT1 Affects Iron Translocation in Rice Plants Exposed to Iron Toxicity |
| title_fullStr | Potassium Ion Channel Gene OsAKT1 Affects Iron Translocation in Rice Plants Exposed to Iron Toxicity |
| title_full_unstemmed | Potassium Ion Channel Gene OsAKT1 Affects Iron Translocation in Rice Plants Exposed to Iron Toxicity |
| title_short | Potassium Ion Channel Gene OsAKT1 Affects Iron Translocation in Rice Plants Exposed to Iron Toxicity |
| title_sort | potassium ion channel gene osakt1 affects iron translocation in rice plants exposed to iron toxicity |
| topic | abiotic stress iron toxicity potassium potassium ion channel rice tolerance |
| url | https://www.frontiersin.org/article/10.3389/fpls.2019.00579/full |
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