Droplet barcoding for massively parallel single-molecule deep sequencing
The ability to accurately sequence long DNA molecules is important across biology. Here, Lan et al. report a droplet-based method that barcodes single DNA molecules, allowing the full-length molecules to be sequenced with multi-fold coverage using short-read next-generation sequencing.
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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Nature Portfolio
2016-06-01
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| Series: | Nature Communications |
| Online Access: | https://doi.org/10.1038/ncomms11784 |
| _version_ | 1818405248385941504 |
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| author | Freeman Lan John R. Haliburton Aaron Yuan Adam R. Abate |
| author_facet | Freeman Lan John R. Haliburton Aaron Yuan Adam R. Abate |
| author_sort | Freeman Lan |
| collection | DOAJ |
| description | The ability to accurately sequence long DNA molecules is important across biology. Here, Lan et al. report a droplet-based method that barcodes single DNA molecules, allowing the full-length molecules to be sequenced with multi-fold coverage using short-read next-generation sequencing. |
| first_indexed | 2024-12-14T08:53:02Z |
| format | Article |
| id | doaj.art-4515e37d471a4e2ab6daee8b7d910e97 |
| institution | Directory Open Access Journal |
| issn | 2041-1723 |
| language | English |
| last_indexed | 2024-12-14T08:53:02Z |
| publishDate | 2016-06-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Nature Communications |
| spelling | doaj.art-4515e37d471a4e2ab6daee8b7d910e972022-12-21T23:08:59ZengNature PortfolioNature Communications2041-17232016-06-017111010.1038/ncomms11784Droplet barcoding for massively parallel single-molecule deep sequencingFreeman Lan0John R. Haliburton1Aaron Yuan2Adam R. Abate3Department of Bioengineering and Therapeutic Sciences, California Institute for Quantitative Biosciences (QB3), University of CaliforniaDepartment of Bioengineering and Therapeutic Sciences, California Institute for Quantitative Biosciences (QB3), University of CaliforniaDepartment of Bioengineering and Therapeutic Sciences, California Institute for Quantitative Biosciences (QB3), University of CaliforniaDepartment of Bioengineering and Therapeutic Sciences, California Institute for Quantitative Biosciences (QB3), University of CaliforniaThe ability to accurately sequence long DNA molecules is important across biology. Here, Lan et al. report a droplet-based method that barcodes single DNA molecules, allowing the full-length molecules to be sequenced with multi-fold coverage using short-read next-generation sequencing.https://doi.org/10.1038/ncomms11784 |
| spellingShingle | Freeman Lan John R. Haliburton Aaron Yuan Adam R. Abate Droplet barcoding for massively parallel single-molecule deep sequencing Nature Communications |
| title | Droplet barcoding for massively parallel single-molecule deep sequencing |
| title_full | Droplet barcoding for massively parallel single-molecule deep sequencing |
| title_fullStr | Droplet barcoding for massively parallel single-molecule deep sequencing |
| title_full_unstemmed | Droplet barcoding for massively parallel single-molecule deep sequencing |
| title_short | Droplet barcoding for massively parallel single-molecule deep sequencing |
| title_sort | droplet barcoding for massively parallel single molecule deep sequencing |
| url | https://doi.org/10.1038/ncomms11784 |
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